Burkholderia cepacia infection of the prostate caused by inoculation ofcontaminated ultrasound gel during transrectal biopsy of the prostate

Organ, Michael; Grantmyre, John; Hutchinson, Jim

doi:10.5489/cuaj.857

Cited by 15 publications

(14 citation statements)

References 9 publications

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“…In this cohort of male UCPPS patients, we were not able to show a clear clinically significant difference in the microbiome (either individual microorganisms or groups of microorganisms) between UCPPS participants and control participants without UCPPS symptoms. We noted specific microbiome differences for Burkholderia cenocepacia (more prevalent in VB1 in UCPPS participants compared to controls) and others have described this organism as a pathogen 22 , possibly involved in the etiology of CP/CPPS 23,24 . The minor differences observed in VB1 for Propionibacterium acnes and Staphylococcus capitis/capare (both under represented in UCPPS participants compared to controls) may be clinically insignificant, but they could also indicate a change in the overall species balance.…”

Section: Discussionsupporting

confidence: 56%

Search for Microorganisms in Men with Urologic Chronic Pelvic Pain Syndrome: A Culture-Independent Analysis in the MAPP Research Network

et al. 2015

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Introduction We used next-generation, state-of-the-art, culture-independent methodology to survey urine microbiota of UCPPS males and control participants enrolled in the MAPP Network to investigate a possible microbial etiology. Methods Male UCPPS patients and matched controls were asked to provide VB1, VB2 and VB3 urine specimens. Specimens were analyzed with Ibis T-5000 Universal Biosensor technology to provide comprehensive identification of bacterial and select fungal species. Differences between UCPPS and control study participants for presence of species or species variation within a higher taxonomic grouping (genus) were evaluated using permutational multivariate analysis of variance and logistic regression. Results VB1 and VB2 urine specimens were obtained from 110 (VB3 in 67) UCPPS participants and 115 (VB3 in 62) controls. A total of 78, 73 and 54 species (42, 39 and 27 genera) were detected in VB1, VB2 and VB3 respectively. Mean (SD) VB1, VB2 and VB3 species count per person was 1.62 (1.28), 1.38 (1.36) and 1.33(1.24) for cases and 1.75(1.32), 1.23(1.15) and 1.56 (0.97) for controls respectively. Overall species and genus composition differed significantly between UCPPS and control participants in VB1 (p=0.002 species level, p=0.004 genus level) with Burkholderia cenocepacia over represented in UCPPS cases. No significant differences were observed at any level in VB2 or VB3 samples. Conclusions Assessment of baseline culture-independent microbiological data from male subjects enrolled in the MAPP Network has identified over representation of B cenocepacia in UCPPS. Future studies are planned to further evaluate microbiota associations with variable and changing UCPPS symptom patterns.

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Section: Discussionsupporting

confidence: 56%

Search for Microorganisms in Men with Urologic Chronic Pelvic Pain Syndrome: A Culture-Independent Analysis in the MAPP Research Network

et al. 2015

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“…Environmental flora, mainly composed of non-fermentative Gram-negative bacilli is responsible for nosocomial infections in debilitated patients. Outbreaks due to P. aeruginosa [26] , [27] , Burkholderia cepacia [28] , [29] , Achromobacter xylosoxidans [30] and recently multidrug resistant bacteria [31] have been increasingly associated with transrectal ultrasonography. These data indicate that full consideration of bacterial contamination of endocavity ultrasound probes is essential.…”

Section: Resultsmentioning

confidence: 99%

Persistence of Microbial Contamination on Transvaginal Ultrasound Probes despite Low-Level Disinfection Procedure

et al. 2014

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Aim of the StudyIn many countries, Low Level Disinfection (LLD) of covered transvaginal ultrasound probes is recommended between patients' examinations. The aim of this study was to evaluate the antimicrobial efficacy of LLD under routine conditions on a range of microorganisms.Materials and MethodsSamples were taken over a six month period in a private French Radiology Center. 300 specimens derived from endovaginal ultrasound probes were analyzed after disinfection of the probe with wipes impregnated with a quaternary ammonium compound and chlorhexidine. Human papillomavirus (HPV) was sought in the first set of s100 samples, Chlamydia trachomatis and mycoplasmas were searched in the second set of 100 samples, bacteria and fungi in the third 100 set samples. HPV, C. trachomatis and mycoplasmas were detected by PCR amplification. PCR positive samples were subjected to a nuclease treatment before an additional PCR assay to assess the likely viable microorganisms. Bacteria and fungi were investigated by conventional methods.ResultsA substantial persistence of microorganisms was observed on the disinfected probes: HPV DNA was found on 13% of the samples and 7% in nuclease-resistant form. C. trachomatis DNA was detected on 20% of the probes by primary PCR but only 2% after nuclease treatment, while mycoplasma DNA was amplified in 8% and 4%, respectively. Commensal and/or environmental bacterial flora was present on 86% of the probes, occasionally in mixed culture, and at various levels (10->3000 CFU/probe); Staphylococcus aureus was cultured from 4% of the probes (10-560 CFU/probe). No fungi were isolated.ConclusionOur findings raise concerns about the efficacy of impregnated towels as a sole mean for disinfection of ultrasound probes. Although the ultrasound probes are used with disposable covers, our results highlight the potential risk of cross contamination between patients during ultrasound examination and emphasize the need for reviewing the disinfection procedure.

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“…Ultrasound procedures contaminated with pathogenic bacteria can cause disease and may lead to further spread of these organisms. 7,9,10,[14][15][16][18][19][20][21] Of particular concern are the following: 22 Blood-borne viruses (BBVs) such as the human immunodeficiency virus (HIV), Hepatitis B virus (HBV) and Hepatitis C virus (HCV) can be spread through contact with the blood or body fluid of infected persons. Many chemical disinfectants inactivate blood-borne viruses; however, ineffective cleaning prior to disinfection limits the effectiveness of chemical disinfection and leads to the persistence of active virus after a disinfection procedure.…”

Section: Agents Potentially Transmitted By Ultrasound Proceduresmentioning

confidence: 99%

“…Failure to adhere to minimum infection control standards, including the proper cleaning and reprocessing of the equipment and transducers, increases the risk of pathogen transmission and subsequent infection. Lack of compliance with scientifically based guidelines for infection control has led to numerous outbreaks arising from ultrasound examinations, [2][3][4][5][6][7][8][9][10] including cases of infection resulting from ultrasound-guided procedures, 4,[11][12][13] and ultrasound transducers that have not undergone appropriate disinfection (Medical Device Alert Ref:…”

Section: Introductionmentioning

confidence: 99%

Guidelines for Reprocessing Ultrasound Transducers

Basseal¹,

Westerway²,

Juraja³

et al. 2017

Australas J Ultrason Med

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The Australasian Society for Ultrasound in Medicine (ASUM) is the leading multidisciplinary medical ultrasound society advancing the clinical practice of diagnostic medical ultrasound for the highest standards of patient care in Australia and New Zealand. The Australasian College for Infection Prevention and Control (ACIPC) is the peak body for Infection Prevention and Control professionals in the Australasian region focused on promoting education and evidence based practice outcomes for a healthy community. This document was developed collaboratively by ASUM and ACIPC to establish nationally accepted guidelines for reprocessing ultrasound transducers. The requirements in these guidelines have been based on the standards of AS/ NZS4187:2014 and AS/NZS4185:2006. 1 These guidelines must be used as the minimum standard of practice for reprocessing ultrasound transducers and considered to be best practice at the time which they were issued.

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Burkholderia cepacia infection of the prostate caused by inoculation ofcontaminated ultrasound gel during transrectal biopsy of the prostate

Cited by 15 publications

References 9 publications

Search for Microorganisms in Men with Urologic Chronic Pelvic Pain Syndrome: A Culture-Independent Analysis in the MAPP Research Network

Search for Microorganisms in Men with Urologic Chronic Pelvic Pain Syndrome: A Culture-Independent Analysis in the MAPP Research Network

Persistence of Microbial Contamination on Transvaginal Ultrasound Probes despite Low-Level Disinfection Procedure

Guidelines for Reprocessing Ultrasound Transducers

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