Burkholderia cenocepacia and Burkholderia multivorans: influence on survival in cystic fibrosis

Jones, Andrew M.; Dodd, Mary; Govan, John R. W.; Barcus, Victoria A.; Doherty, C.; Morris, J.; Webb, A K

doi:10.1136/thx.2003.017210

Cited by 230 publications

(218 citation statements)

References 18 publications

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“…The BCC is also established as a frequent cause of chronic respiratory infection in patients with cystic fibrosis (CF), and is consistently identified as an independent risk factor for mortality within this patient group (Liou et al, 2001;Jones et al, 2004;Kalish et al, 2006). Although most commonly causing a gradual deterioration in lung function like other CF pathogens, BCC can also uniquely cause systemic CF infection termed cepacia syndrome, characterized by necrotizing pneumonia, bacteraemia and sepsis (Isles et al, 1984).…”

Section: Introductionmentioning

confidence: 99%

Growth on mannitol-rich media elicits a genome-wide transcriptional response in Burkholderia multivorans that impacts on multiple virulence traits in an exopolysaccharide-independent manner

et al. 2014

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In common with other members of the Burkholderia cepacia complex (BCC), Burkholderia multivorans is capable of producing exopolysaccharide (EPS) when grown on certain mannitolrich media. The significance of the resulting mucoid phenotype and the genome-wide response to mannitol has never been characterized despite its clinical relevance following the approval of a dried-powder preparation of mannitol as an inhaled osmolyte therapy for cystic fibrosis (CF) patients. In the present study we defined the transcriptional response of B. multivorans ATCC 17616, a model genome-sequenced strain of environmental origin, to growth on mannitol-rich yeast extract media (MYEM). EPS-dependent and -independent impact of MYEM on virulenceassociated traits was assessed in both strain ATCC 17616 and the CF isolate B. multivorans C1576. Our studies revealed a significant transcriptional response to MYEM encompassing approximately 23 % of predicted genes within the genome. Strikingly, this transcriptional response identified that EPS induction occurs in ATCC 17616 without the upregulation of the bce-I and bce-II EPS gene clusters, despite their pivotal role in EPS biosynthesis. Of approximately 20 differentially expressed putative virulence factors, 16 exhibited upregulation including flagella, ornibactin, oxidative stress proteins and phospholipases. MYEM-grown B. multivorans also exhibited enhanced motility, biofilm formation and epithelial cell invasion. In contrast to these potential virulence enhancements, MYEM-grown B. multivorans C1576 showed attenuated virulence in the Galleria mellonella infection model. All of the observed phenotypic responses occurred independently of EPS production, highlighting the profound impact that mannitol-based growth has on the physiology and virulence of B. multivorans.

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Section: Introductionmentioning

confidence: 99%

Growth on mannitol-rich media elicits a genome-wide transcriptional response in Burkholderia multivorans that impacts on multiple virulence traits in an exopolysaccharide-independent manner

et al. 2014

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“…The patient was felt to have "cepacia syndrome," characterized by persistently elevated inflammatory markers, leukocytosis, intermittent fevers, and recurrent petechial skin eruption (shown to be leukocytoclastic vasculitis on skin biopsy), although his blood cultures did not become positive until a later date. This condition, well described in published case series, is recognized to be uniformly fatal (1,9). Thus, a sense of urgency developed.…”

Section: Treatment and Outcomementioning

confidence: 87%

“…Pulmonary infection with Burkholderia cenocepacia, a species within the Burkholderia cepacia complex, raises concerns for both CF clinicians and patients alike, given its association with increased lung function decline and earlier mortality (1,(15)(16)(17). B. cenocepacia is perhaps best known as the most frequent cause of "cepacia syndrome," a necrotizing pneumonia with septicemia resulting in rapid clinical decline and, frequently, death (4).…”

Section: Commentarymentioning

confidence: 99%

“…Infection with Bcc leads to a spectrum of clinical manifestations, ranging from asymptomatic carriage in respiratory secretions to necrotizing pneumonia (1,3,4). Burkholderia cenocepacia isolates are frequently virulent, transmissible, and innately resistant to multiple drug classes (1,5). No standardized regimens have been developed for treatment of B. cenocepacia infection (3).…”

Section: N the Cystic Fibrosis (Cf) Patient Population Infection Withmentioning

confidence: 99%

“…Burkholderia cepacia complex (Bcc) is uncommon but important, due to the accelerated decline in lung function and the increased mortality in affected individuals (1). Bcc consists of 18 different genetically related Gram-negative, non-spore-forming species (2).…”

Section: N the Cystic Fibrosis (Cf) Patient Population Infection Withmentioning

confidence: 99%

See 2 more Smart Citations

Clinafloxacin for Treatment of Burkholderia cenocepacia Infection in a Cystic Fibrosis Patient

Balwan

Nicolau

Wungwattana

et al. 2016

Antimicrob Agents Chemother

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Antibiotic treatment for Burkholderia cepacia complex in people with cystic fibrosis experiencing a pulmonary exacerbation

Horsley¹,

Jones²

2011

Cochrane Database of Systematic Reviews

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Burkholderia cenocepacia and Burkholderia multivorans: influence on survival in cystic fibrosis

Cited by 230 publications

References 18 publications

Growth on mannitol-rich media elicits a genome-wide transcriptional response in Burkholderia multivorans that impacts on multiple virulence traits in an exopolysaccharide-independent manner

Growth on mannitol-rich media elicits a genome-wide transcriptional response in Burkholderia multivorans that impacts on multiple virulence traits in an exopolysaccharide-independent manner

Clinafloxacin for Treatment of Burkholderia cenocepacia Infection in a Cystic Fibrosis Patient

Antibiotic treatment for Burkholderia cepacia complex in people with cystic fibrosis experiencing a pulmonary exacerbation

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