Burkholderia anthina sp. nov. and Burkholderia pyrrocinia, two additional Burkholderia cepacia complex bacteria, may confound results of new molecular diagnostic tools

Vandamme, Peter; Henry, Deborah A.; Coenye, Tom; Nzula, Sazini; Vancanneyt, Marc; LiPuma, John J.; Speert, David P.; Govan, John R. W.; Mahenthiralingam, Eshwar

doi:10.1016/s0928-8244(02)00301-2

Cited by 113 publications

(119 citation statements)

References 23 publications

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“…Phenotypic identification and antibiotic susceptibility tests were carried out in the Microscan system (Baxter). Identification of B. cepacia complex bacteria was confirmed by wholecell protein electrophoresis (Pot et al, 1994) and recA restriction fragment length polymorphism (RFLP) analysis (Mahenthiralingam et al, 2000;Vandamme et al, 2002).…”

Section: Methodsmentioning

confidence: 99%

Characterizing uncommon Burkholderia cepacia complex isolates from an outbreak in a haemodialysis unit

Souza

Moreira

Pasternak

et al. 2004

Journal of Medical Microbiology

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An outbreak of bacteraemia in a haemodialysis unit where 65 episodes of infection involved 35 outpatients is reported. Burkholderia cepacia complex was the agent most frequently recovered from blood. Thirty-three environmental and clinical isolates of B. cepacia complex were characterized by whole-cell protein electrophoresis and recA-RFLP profile. Fourteen isolates were genomovar I and 16 isolates were not classifiable by their recA-RFLP pattern. Ribotyping, random amplification of polymorphic DNA (RAPD) and integron profile were used to explore the clonality of the isolates, and revealed multiple strain genotypes. Four ribotypes and RAPD types and three integron patterns were identified. The water supply was identified as the source of the outbreak, and inappropriate cleaning and a leak in the reverse osmosis tubing connection were the probable causes of contamination. B. cepacia complex was still recovered from blood of patients even after apparently adequate measures were taken and water quality standards were met, suggesting that higher standards for water quality should be adopted in haemodialysis units. The genomovars recovered here were distinct from those commonly reported for cystic fibrosis isolates.

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Section: Methodsmentioning

confidence: 99%

Characterizing uncommon Burkholderia cepacia complex isolates from an outbreak in a haemodialysis unit

Souza

Moreira

Pasternak

et al. 2004

Journal of Medical Microbiology

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show abstract

“…[69][70][71][72][73][74][75][76][77][78][79][80] The frequency of detection of these species in people with CF varies considerably, although Burkholderia cenocepacia and Burkholderia multivorans are most common. 26,81 Burkholderia gladioli is the third most frequently isolated Burkholderia species among CF patients in the United States.…”

Section: Ic2 Burkholderia Sppmentioning

confidence: 99%

Infection Prevention and Control Guideline for Cystic Fibrosis: 2013 Update

Saiman

Siegel

LiPuma

et al. 2014

Infect. Control Hosp. Epidemiol.

344

382

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The 2013 Infection Prevention and Control (IP&C) Guideline for Cystic Fibrosis (CF) was commissioned by the CF Foundation as an update of the 2003 Infection Control Guideline for CF. During the past decade, new knowledge and new challenges provided the following rationale to develop updated IP&C strategies for this unique population:1. The need to integrate relevant recommendations from evidence-based guidelines published since 2003 into IP&C practices for CF. These included guidelines from the Centers for Disease Control and Prevention (CDC)/Healthcare Infection Control Practices Advisory Committee (HICPAC), the World Health Organization (WHO), and key professional societies, including the Infectious Diseases Society of America (IDSA) and the Society for Healthcare Epidemiology of America (SHEA). During the past decade, new evidence has led to a renewed emphasis on source containment of potential pathogens and the role played by the contaminated healthcare environment in the transmission of infectious agents. Furthermore, an increased understanding of the importance of the application of implementation science, monitoring adherence, and feedback principles has been shown to increase the effectiveness of IP&C guideline recommendations.2. Experience with emerging pathogens in the non-CF population has expanded our understanding of droplet transmission of respiratory pathogens and can inform IP&C strategies for CF. These pathogens include severe acute respiratory syndrome coronavirus and the 2009 influenza A H1N1. Lessons learned about preventing transmission of methicillin-resistant Staphylococcus aureus (MRSA) and multidrug-resistant gram-negative pathogens in non-CF patient populations also can inform IP&C strategies for CF.

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“…Currently, more than 70 Bcc HaeIII RFLP types have been found (P. Vandamme, unpublished data). Several species include multiple RFLP types (Coenye et al, 2001c;Mahenthiralingam et al, 2000b), and overlap of certain RFLP types is observed in different species (Mahenthiralingam et al, 2000a;McDowell et al, 2001;Vandamme et al, 2002). In particular, isolates showing RFLP types H, J and W should be further investigated using the second restriction enzyme, MnlI, for unambiguous species identification.…”

Section: Introductionmentioning

confidence: 99%

Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex

Vanlaere

LiPuma

Baldwin

et al. 2008

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

222

143

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The taxonomic position of five recA gene clusters of Burkholderia cepacia complex (Bcc) isolates was determined using a polyphasic taxonomic approach, The levels of 16S rRNA and recA gene sequence similarity, multilocus sequence typing (MLST) data and the intermediate DNA-DNA binding values demonstrated that these five clusters represented five novel species within the Bcc. Biochemical identification of these species is difficult, as is the case for most Bcc species. However, identification of these novel species can be accomplished through recA gene sequence analysis, MLST and BOX-PCR profiling and by recA RFLP analysis. For diagnostic laboratories, recA gene sequence analysis offers the best combination of accuracy and simplicity. Based on these results, we propose five novel Bcc species, Burkholderia latens sp. nov. (type strain FIRENZE 3(T) =LMG 24064(T) =CCUG 54555(T)), Burkholderia diffusa sp. nov. (type strain AU1075(T) =LMG 24065(T) =CCUG 54558(T)), Burkholderia arboris sp. nov. (type strain ES0263A(T) =LMG 24066(T) =CCUG 54561(T)), Burkholderia seminalis sp. nov. (type strain AU0475(T) =LMG 24067(T) =CCUG 54564 (T)) and Burkholderia metallica sp. nov. (type strain AU0553(T) =LMG 24068(T) =CCUG 54567(T)). In the present study, we also demonstrate that Burkholderia ubonensis should be considered a member of the Bcc

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Burkholderia anthina sp. nov. and Burkholderia pyrrocinia, two additional Burkholderia cepacia complex bacteria, may confound results of new molecular diagnostic tools

Cited by 113 publications

References 23 publications

Characterizing uncommon Burkholderia cepacia complex isolates from an outbreak in a haemodialysis unit

Characterizing uncommon Burkholderia cepacia complex isolates from an outbreak in a haemodialysis unit

Infection Prevention and Control Guideline for Cystic Fibrosis: 2013 Update

Burkholderia latens sp. nov., Burkholderia diffusa sp. nov., Burkholderia arboris sp. nov., Burkholderia seminalis sp. nov. and Burkholderia metallica sp. nov., novel species within the Burkholderia cepacia complex

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