Burden of rotavirus and other enteropathogens among children with diarrhea in Burkina Faso

Nitiema, Léon W.; Nordgren, Johan; Ouermi, Djénèba; Dianou, Dayéri; Traoré, Alfred S.; Svensson, Lennart; Simporé, Jacques

doi:10.1016/j.ijid.2011.05.009

Cited by 107 publications

(140 citation statements)

References 39 publications

(51 reference statements)

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“…5) (Kotloff et al, 2013). This finding is consistent with results from several epidemiological studies performed in developing countries such as Burkina Faso, Nigeria, Rwanda, Bangladesh, New Caledonia and Vietnam (Albert et al, 1999;Bonkoungou et al, 2013;Germani et al, 1994;Kabayiza et al, 2014a;Nitiema et al, 2011;Ogunsanya et al, 1994;Vu Nguyen et al, 2006). In another review of enteropathogens associated with diarrhoea in developing countries, rotavirus, enteropathogenic and toxigenic E. coli were found to be the main agents among diarrhoea-causing pathogens (O'Ryan et al, 2005).…”

Section: Major Causative Agents Of Diarrhoea In Developing Countriessupporting

confidence: 86%

Role of noroviruses as aetiological agents of diarrhoea in developing countries

Ayukekbong

Mesumbe²,

Oyero

et al. 2015

Journal of General Virology

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Section: Major Causative Agents Of Diarrhoea In Developing Countriessupporting

confidence: 86%

Role of noroviruses as aetiological agents of diarrhoea in developing countries

Ayukekbong

Mesumbe²,

Oyero

et al. 2015

Journal of General Virology

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“…More than 80% of rotavirus-positive children presented with diarrhea. These results are similar to those reported for several industrialized and nonindustrialized countries, including Brazil 3,13,14,18 . Despite the fact that a similar virus distribution was observed throughout the study period, our data suggest a decrease in rotavirus infection rates.…”

supporting

confidence: 90%

Shift in Human Rotavirus Distribution in Belo Horizonte, Brazil Detected by Ribonucleic Acid Electrophoresis

Silva

Penna

Duarte

et al. 2013

Rev. Inst. Med. trop. S. Paulo

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SUMMARYRotavirus has been considered the main agent of infectious diarrhea especially among younger children. We addressed the prevalence of rotavirus-associated diarrhea and the diversity of circulating electropherotypes by immunochromatography and RNA electrophoresis. Stool samples were taken from 391 children (267 with diarrhea) from the lower socioeconomic stratum who sought treatment in the Hospital Infantil João Paulo II/Belo Horizonte, during 2005 and 2006. Rotavirus was detected in 79/20.2% of subjects, 64/24.0% with diarrhea and 15/12.1% with no diarrhea. The virus was strongly associated with diarrhea (p = 0.003). A total of 76/19.4% and 69/17.6% rotavirus-positive children were identified by immunochromatography and electrophoresis, respectively. Rotavirus-associated diarrhea was more frequently detected in dry months (p < 0.001) and almost exclusively in children aged up to three years. Long profile strains prevailed (54/78.3%) but a shift toward short electropherotype was identified. Despite the decrease seen in 2006, rotavirus infection is still very common in our area. Although viral RNA electrophoresis is useful as a typing method, it should not be used exclusively in the diagnosis of rotavirus infection. We confirmed a shift from long to short profile strains, as already described for other South American countries.

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“…These methods provide better identification of viruses that cannot be cultured (e.g., Caliciviridae) or that previously have been diagnosed with methods with relatively low sensitivity (e.g., antigen testing for rotavirus) (8)(9)(10). They also have been shown to improve the detection of bacteria because of their higher sensitivity than culture (11)(12)(13)(14)(15).…”

mentioning

confidence: 99%

Real-Time PCR Threshold Cycle Cutoffs Help To Identify Agents Causing Acute Childhood Diarrhea in Zanzibar

et al. 2014

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f Molecular assays might improve the identification of causes of acute diarrheal disease but might lead to more frequent detection of asymptomatic infections. In the present study, real-time PCR targeting 14 pathogens was applied to rectal swabs from 330 children aged 2 to 59 months in Zanzibar, including 165 patients with acute diarrhea and 165 asymptomatic control subjects. At least one pathogen was detected for 94% of the patients and 84% of the controls, with higher rates among patients for norovirus genogroup II (20% versus 2.4%; P < 0.0001), rotavirus (10% versus 1.8%; P ‫؍‬ 0.003), and Cryptosporidium (30% versus 11%; P < 0.0001). Detection rates did not differ significantly for enterotoxigenic Escherichia coli (ETEC)-estA (33% versus 24%), ETEC-eltB (44% versus 46%), Shigella (35% versus 33%), and Campylobacter (35% versus 33%), but for these agents threshold cycle (C T ) values were lower (pathogen loads were higher) in sick children than in controls. In a multivariate analysis, C T values for norovirus genogroup II, rotavirus, Cryptosporidium, ETEC-estA, and Shigella were independently associated with diarrhea. We conclude that this real-time PCR allows convenient detection of essentially all diarrheagenic agents and provides C T values that may be critical for the interpretation of results for pathogens with similar detection rates in patients and controls. The results indicate that the assessment of pathogen loads may improve the identification of agents causing gastroenteritis in children.

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Burden of rotavirus and other enteropathogens among children with diarrhea in Burkina Faso

Abstract: This study demonstrates rotavirus as the main etiological agent in pediatric diarrhea in Burkina Faso, and further shows the great severity of rotavirus-induced diarrhea in undernourished children in Burkina Faso.

Cited by 107 publications

References 39 publications

Role of noroviruses as aetiological agents of diarrhoea in developing countries

Role of noroviruses as aetiological agents of diarrhoea in developing countries

Shift in Human Rotavirus Distribution in Belo Horizonte, Brazil Detected by Ribonucleic Acid Electrophoresis

Real-Time PCR Threshold Cycle Cutoffs Help To Identify Agents Causing Acute Childhood Diarrhea in Zanzibar

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