Bullous pemphigoid: from the bedside to the research laboratory

Ghohestani, Reza F.; Novotney, Jennifer; Chaudhary, Meena; Agah, Roya S.

doi:10.1016/s0738-081x(00)00194-2

Cited by 15 publications

(18 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These antigens are not expressed, however, along the GBM (Ghohestani et al, 2000). In BP, characterized by subepidermal blisters, IgG autoantibodies are directed against the 180-and 230-kDa BP antigens (Ghohestani et al, 2001b;Stern, 2002;Yancey and Egan, 2000). CP rarely exhibits widespread skin lesions, and if observed, they are accompanied by scarring; the antibodies in CP are targeted to the 180-kDa BP antigen, laminin-5, or an uncharacterized 168-kDa molecule (Cotell et al, 2000;Egan and Yancey, 2000).…”

Section: Discussionmentioning

confidence: 99%

Crescentic Glomerulonephritis and Subepidermal Blisters with Autoantibodies to α5 and α6 Chains of Type IV Collagen

Ghohestani

Rotunda

Hudson

et al. 2003

Laboratory Investigation

View full text Add to dashboard Cite

SUMMARY:We describe a novel autoimmune disease characterized by severe subepidermal bullous eruption and crescentic glomerulonephritis with autoantibodies directed against the noncollagenous domain of the ␣5 and ␣6 chains of type IV collagen. Biopsy of perilesional skin revealed a subepidermal blister with marked polymorphonuclear infiltrate with linear deposits of IgA and C3. Light microscopy of a kidney biopsy specimen revealed a crescentic glomerulonephritis, and immunofluorescence microscopy showed linear basement membrane staining for IgA (3ϩ), C3 (1ϩ), and IgG (1ϩ). No electron-dense deposits were observed by transmission electron microscopy. The patient's autoantibodies reacted with normal human skin and kidney: IgA (3ϩ) and IgG (1ϩ) antibodies stained the basement membrane zones of skin, renal glomerulus, and some tubules. The identity of the target antigen was determined by immunochemical analyses of candidate antigens using the patient's autoantibodies. The patient's IgA and IgG autoantibodies reacted with a 185-to 190-kDa antigen from a human dermal extract that was distinguished from the other dermal or epidermal antigens, including the 145-to 290-kDa (type VII collagen) epidermolysis bullosa acquisita antigen, the 165-to 200-kDa ␣3 laminin mucous membrane cicatricial pemphigoid antigen, and the 230-kDa and the 180-kDa bullous pemphigoid antigens. Patient's IgA and IgG autoantibodies further reacted with the ␣5(IV) and weakly with the ␣6(IV) chains of type IV collagen by Western blot and ELISA. This report expands the repertoire of bullous skin disorders and provides an explanation for the association of anti-type IV collagen autoantibodies and glomerulonephritis with subepidermal blisters. (Lab Invest 2003, 83:605-611).

show abstract

Section: Discussionmentioning

confidence: 99%

Crescentic Glomerulonephritis and Subepidermal Blisters with Autoantibodies to α5 and α6 Chains of Type IV Collagen

Ghohestani

Rotunda

Hudson

et al. 2003

Laboratory Investigation

View full text Add to dashboard Cite

show abstract

“…However, sometimes blisters cannot be observed throughout the whole course of the disease, making diagnosis substantially difficult. Many authors have defined this particular form of BP pemphigoid nodularis (PN) [25][26][27][28][29][30][31][32][33][34] Fig. 1.…”

Section: Atypical Presentations Of Bullous Pemphigoidmentioning

confidence: 99%

Atypical presentations of bullous pemphigoid: Clinical and immunopathological aspects

Cozzani

Gasparini

Burlando

et al. 2015

Autoimmunity Reviews

View full text Add to dashboard Cite

“…25,26 The peaks observed in the amide I region, with wavenumbers of 1562 and 1606 cm 1 , are characteristic of tryptophan and tyrosin (ring stretches), 25 1582 cm 1 of aspartic and glutamic acid 27 phenylalanine and tyrosine. 25 The role of IgG and fibrin in BP 15,17 is further supported by the appearance of the peaks corresponding to their components and structural changes.…”

Section: Healthy Skinmentioning

confidence: 90%

“…IgG is directed against components of the epidermal basement membrane zone and specific for the hemidesmosomal BP antigens BP230 (BPAg1) and BP180 (BPAg2). 14,15 Indirect immunofluorescence tests detected circulating antibasement membrane zone IgG antibodies in 70-80% of BP cases. Direct immunofluorescence (DIF) studies of perilesional skin revealed linear deposition of IgG (65-95% of cases) as well as C3 (100%) at the dermoepidermal junction (DEJ).…”

Section: Introductionmentioning

confidence: 99%

“…16 In the lumen of the blister fibrin, lymphocytes, eosinophils and neutrophils are present at different densities (cell-poor or cell-rich variants). 15,16 It has been suggested that the sequence of pathogenic events inducing lamina lucida separation involves increased microvascular permeability, leading to papillary edema and fibrin deposition. 17 The present study is aimed at evaluating whether it is possible to distinguish by Raman spectroscopy between healthy and diseased skin in patients with BP.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Bullous pemphigoid detection by micro-Raman spectroscopy and cluster analysis: structure alterations of proteins

Azrad

Cagnano

Halevy

et al. 2005

J. Raman Spectrosc.

View full text Add to dashboard Cite

In this study, micro-Raman spectroscopy was used, for the first time, to detect spectral changes between healthy and diseased skin tissues with bullous pemphigoid (BP). The spectral changes provide information about the biochemical alterations between normal skin and blistered and nonblistered regions in samples diagnosed histopathologically as BP. Raman spectra, characterized by many peaks, revealed the molecular composition of the different skin layers, stratum corneum, epidermis and dermis of normal skin. Comparison of spectra monitored at the dermoepidermal junction (DEJ) of healthy skin with those of blisters caused by BP showed evidence for large variations in the amide I and III regions. The alterations of the protein content, amide I and III, are a result of the appearance of immunoglobulin G (IgG) and fibrin, characteristics of BP. Indication for the disease at early stages was obtained from changes in protein content, evidenced in the measured spectra assisted by cluster analysis. The method employed here can contribute to the nearly real-time diagnosis and to a better understanding of the physical and biomolecular processes effected by BP, and might have implications on other skin diseases.

show abstract

Bullous pemphigoid: from the bedside to the research laboratory

Cited by 15 publications

References 46 publications

Crescentic Glomerulonephritis and Subepidermal Blisters with Autoantibodies to α5 and α6 Chains of Type IV Collagen

Crescentic Glomerulonephritis and Subepidermal Blisters with Autoantibodies to α5 and α6 Chains of Type IV Collagen

Atypical presentations of bullous pemphigoid: Clinical and immunopathological aspects

Bullous pemphigoid detection by micro-Raman spectroscopy and cluster analysis: structure alterations of proteins

Contact Info

Product

Resources

About