Bullera taiwanensis sp. nov. and Bullera formosensis sp. nov., two new ballistoconidium-forming yeast species isolated from plant leaves in Taiwan.

Abstract

Two strains of ballistoconidiogenous yeasts that contain xylose and form Q-10 ubiquinone were isolated from plant leaves collected in Taiwan and were found to represent two new species. The taxonomic properties of both coincide with the genus Bullera so they are described as Bullera taiwanensis sp. nov. and Bullera formosensis sp. nov. In a phylogenetic tree based on the nucleotide sequences of 18S ribosomal DNAs, these two species are distant from the clusters where the remaining members of the genus Bullera are located, i.e., Bullera taiwanensis is located in the Filobasidium lineage (Filobasidiales clade) and Bullera formosensis is located in the Cryptococcus humicola-Trichosporon lineage (Trichosporonales clade).Key Words--Bullera formosensis sp. nov.; Bullera taiwanensis sp. nov.; new ballistoconidiogenous yeasts; new yeasts from Taiwan

Bullera koratensis sp. nov. and Bullera lagerstroemiae sp. nov., two new ballistoconidium-forming yeast species in the Trichosporonales clade isolated from plant leaves in Thailand

“…These strains can be discriminated from B. formosensis by differences in 18S, the D1/D2 domain and ITS sequences. Besides this, the difference in mol% GϩC between B. formosensis (57.8 mol%, Nakase et al, 2002) and TY-242 (49.4 mol%) confirm that the six strains represent a new species different from B. formosensis.…”

Cryptococcus silvicola nov. sp. from nature reserves of Russia and Portugal

“…phenolicus, but differs from this species by the ability to grow at 30°C and in vitamin-free medium. (Figure 1), a recently described species and the sole filobasidiaceous yeast in the genus Bullera Derx (Nakase et al 2002), was not anticipated. Almost all members of this genus are located in the Tremellales clade.…”

Sporobolomyces fushanensis sp. nov., a new species of ballistoconidium-forming yeast in the Microbotryum lineage isolated from a plant in Taiwan

“…However, only a few reports have been published on yeasts living in the phyllosphere of Taiwan (Nakase et al, 2002(Nakase et al, , 2003(Nakase et al, , 2004. In the course of a survey of yeasts in the phyllosphere of the Asian region, we found a yeast strain coinciding with the genus Sporobolomyces from a plant in Taiwan.…”

“…Cellular xylose was analyzed by HPLC as described by Suzuki and Nakase (1988). The 18S rDNA and the D1/D2 region of 26S rDNA were directly sequenced using PCR products by ABI 310 sequencer with an ABI Prism BigDye Terminator Cycle Sequence kit (Applied Biosystems, Stafford, USA) as previously described (Nakase et al, 2002 (Thompson et al, 1997). The evolutionary distances for the neighbor-joining method (Saitou and Nei, 1987) were calculated according to Kimura (1980).…”