Bull subfertility is associated with low levels of a sperm membrane antigen

Parent, Sophie; Lefièvre, Linda; Brindle, Yves; Sullivan, Robert

doi:10.1002/(sici)1098-2795(199901)52:1<57::aid-mrd8>3.0.co;2-u

Cited by 61 publications

(41 citation statements)

References 44 publications

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“…Bull (Ollero et al 1998) and boar (Huang et al 2009) sperm surface proteins, collected before and after freeze-thawing, showed processing related differences using comparative SDS-PAGE analysis, but the effect of these protein alterations on sperm function remains unknown. The intensity of a fertility-associated protein (P25b; Parent et al 1999) has also been shown to remain stable if bull spermatozoa were stored in liquid nitrogen for !5 days but longer storage periods (O28 days) reduce its abundance compared with that of fresh spermatozoa (Lessard et al 2000). This result is interesting because it suggests that cryo-elution of the protein occurred in an environment where chemically and thermally driven reactions are minimised, and not during cooling or thawing where the majority of damage to the plasma membrane occurs.…”

Section: Sperm Surface Alterations During Sperm Handlingmentioning

confidence: 99%

Sperm surface changes and physiological consequences induced by sperm handling and storage

2011

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Spermatozoa interact with their immediate environment and this contact remodels the sperm surface in preparation for fertilisation. These fundamental membrane changes will be critically covered in this review with special emphasis on the very specific surface destabilisation event, capacitation. This process involves very subtle and intricate modifications of the sperm membrane including removal of suppression (decapacitation) factors and changes in the lateral organisation of the proteins and lipids of the sperm surface. Processing of sperm for assisted reproduction (storage, sex-sorting, etc.) subjects spermatozoa to numerous stressors, and it is possible that this processing overrides such delicate processes resulting in sperm instability and cell damage. To improve sperm quality, novel mechanisms must be used to stabilise the sperm surface during handling. In this review, different types of membrane stress are considered, as well as novel surface manipulation methods to improve sperm stability.

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Section: Sperm Surface Alterations During Sperm Handlingmentioning

confidence: 99%

Sperm surface changes and physiological consequences induced by sperm handling and storage

2011

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“…Moreover, P26h is found in epididymosomes and becomes GPI-anchored to the sperm surface of the acrosomal region during epididymal transit, via an as yet unknown mechanism (Sullivan et al, 2007). While homologous proteins have subsequently been described in man (P34H; Boué et al, 1996), cattle (P25b; Parent et al, 1999) and monkeys (P31m; Lamontagne et al, 2001), it now appears that P26h mRNA and protein are present in relatively large amounts in the testes of sexually mature hamsters whereas the P26h mRNA in the epididymis is much less (Gaudreault et al, 1999). The cellular origin of the P26h on the acrosome of sperm in the cauda epididymis remains, therefore, to be confirmed.…”

Section: Epididymosomes and Their Proteinsmentioning

confidence: 99%

The roles of the epididymis and prostasomes in the attainment of fertilizing capacity by stallion sperm

Šoštarić

Aalberts

Gadella

et al. 2008

Animal Reproduction Science

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The epididymis is a long, tightly coiled tube within the lumen of which sperm matures. Sperm maturation involves morphological and biochemical changes in the sperm plasma membrane in response to epididymal secretions and their various proteins. Some of these proteins become outer membrane components while others become integral membrane proteins; transfer of some proteins to the sperm plasma membrane may be mediated by epididymosomes. Nevertheless, the molecular pathways by which spermatozoa acquire fertilizing capacity during their transit through the epididymis remain ambiguous. In a recent study of stallion epididymal sperm, we found that sperm harvested from different parts of the epididymis (caput, corpus and cauda) had a varying, but generally poor, ability to undergo the acrosome reaction in vitro. At ejaculation, however, sperm mix with seminal plasma which contains various components, including the small membranous vesicles known as prostasomes, that may enable the sperm to undergo physiological activation. Seminal plasma components may have a 'washing' effect and help to remove 'de-capacitation' factors that coat the sperm during storage in the cauda epididymis; alternatively seminal plasma and prostasomes may contain factors that more directly promote sperm activation. This article reviews current information ଝ This paper is part of the Special issue entitled "Proceedings of the 5th International Symposium on Stallion Reproduction", Guest edited by Terttu Katila.* Corresponding author. Author's personal copy 238 E. Sostaric et al. / Animal Reproduction Science 107 (2008) 237-248 on the roles of epididymal and accessory gland fluids on the acquisition of fertilizing capacity by stallion sperm.

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“…Based on their implication in particular steps of the fertilisation process, many sperm components such as lipids (Brinsko et al 2007), proteins (Bellin et al 1998, Parent et al 1999, ions (Collin et al 2000) and nucleic acids (Lalancette et al 2008) have been proposed to vary in quantity or quality according to the male fertility status in many mammalian species. Levels of P25b, a bovine sperm membrane antigen, are lower in semen from subfertile bulls than in the semen from bulls with high fertility (HF) rates (Parent et al 1999). P25b counterparts in human and hamster, P34H and P26h, are involved in zona pellucida (ZP) recognition , Boue et al 1994.…”

Section: Introductionmentioning

confidence: 99%

Proteomic comparison of detergent-extracted sperm proteins from bulls with different fertility indexes

D’Amours

Frenette²,

Fortier³

et al. 2010

Reproduction

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145

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Intrinsic factors such as proteins modulate the fertilising ability of male gametes. We compared detergent-extracted sperm protein composition of bulls with different fertility indexes in order to highlight putative fertility markers of sperm. Frozen semen from 23 Holstein bulls with documented fertility was used. According to their 'fertility solution' (SOL), as calculated by the Canadian dairy network, bulls were divided into four groups: high fertility (HF) (SOLO3.0; nZ6), medium-HF (2.9OSOLO2.0; nZ5), medium-low fertility (K2.8OSOLOK4.9; nZ8) and low fertility (LF; SOL!K5.0; nZ4), with a SOLZ0 being the average. Triton X-100 protein extracts from ejaculated spermatozoa were subjected to two-dimensional difference gel electrophoresis, and polypeptide maps were quantitatively analysed by ImageMaster software. Nine protein spots showed significant differences between the HF and LF groups, and eight of these proteins were identified by liquid chromatography-tandem mass spectrometry. T-complex protein 1 subunits 3 and q (CCT5 and CCT8), two isoforms of epididymal sperm-binding protein E12 (ELSPBP1), proteasome subunit a type-6 and binder of sperm 1 (BSP1) were more expressed in the LF group than in the HF group. On the other hand, adenylate kinase isoenzyme 1 (AK1) and phosphatidylethanolamine-binding protein 1 (PEBP1) were more expressed in the HF group than in the LF group. The presence and expression level of ELSPBP1, BSP1, AK1 and PEBP1 were confirmed by western blot. A linear regression model established that CCT5 and AK1 explained 64% (P!0.001) of the fertility scores. The reported functions of these proteins are in agreement with a putative involvement in defective sperm physiology, where lower or higher levels can jeopardise sperm ability to reach and fertilise the oocyte.

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Bull subfertility is associated with low levels of a sperm membrane antigen

Cited by 61 publications

References 44 publications

Sperm surface changes and physiological consequences induced by sperm handling and storage

Sperm surface changes and physiological consequences induced by sperm handling and storage

The roles of the epididymis and prostasomes in the attainment of fertilizing capacity by stallion sperm

Proteomic comparison of detergent-extracted sperm proteins from bulls with different fertility indexes

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