Bull sperm head morphometry related to abnormal chromatin structure and fertility

Sailer, Brian L.; Jost, Lorna K.; Evenson, Donald P.

doi:10.1002/(sici)1097-0320(19960601)24:2<167::aid-cyto9>3.3.co;2-e

Cited by 14 publications

(16 citation statements)

References 11 publications

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“…It is known that aging induces apoptosis in the epididymis, which seems to be caused by the fall of testosterone levels and is probably related to an increase of oxidative stress in tissues (Jara et al, 2004). It has been stated by Sailer et al (1996) that abnormal chromatin structure may lead to problems in sperm nuclear packaging material, which can be related to morphologically abnormal spermatozoa. This was noticed in our study with respect to young boars, but not in old boars; SCI was also at a high level in this group.…”

Section: Discussionmentioning

confidence: 99%

Age-related changes in quality and fertility of porcine semen

Tsakmakidis

Khalifa²,

Boscos

2012

Biol. Res.

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The aim of this study was to investigate the eff ect of boar age on quality traits and fertility of liquid-stored semen. Boars were allocated into 3 age groups: 7-10 months (young), 18-33 months (mature), 51-61 months (old). Ejaculates of ≥ 200x10 6 sperm/ml and 85% total motile sperm were extended to 30x10 6 sperm/ml, stored at 17-18 °C and used within 12-24 h for artifi cial insemination (AI) of 2062 multiparous sows. After 24 h of storage, aliquots of diluted semen were assessed for sperm progressive motility (SPM), incidence of sperm chromatin instability (SCI), proportion of live morphologically normal sperm (LMNS) and head morphometry of LMNS. The results showed that young boars had higher percentages of SCI and lower proportions of LMNS than those of the mature (p < 0.05) and old (p < 0.001) boars, respectively. Sperm head dimensions of young and old boars were greater (p < 0.03-0.001) than those of mature boars. The farrowing rate of young boars (65%) was signifi cantly lower (p < 0.001; χ 2 = 30-61) than those of the mature (87.2%) and old (84.7%) boars. The relationship between sperm head dimensions and boar fertility was non-signifi cant. In conclusion, boar age is an important physiological factor contributing to the success of swine AI.

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Section: Discussionmentioning

confidence: 99%

Age-related changes in quality and fertility of porcine semen

Tsakmakidis

Khalifa²,

Boscos

2012

Biol. Res.

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“…Further, any subtle changes in sperm chromatin condensation or alteration in sperm histone-protamine ratio necessary for chromatin packing may directly reflect in changes in head morphology and morphometry [10]. Experimental studies have demonstrated a positive correlation between abnormal sperm head morphology and DNA damage and chromatin integrity, but such relationship in infertile patients was not extensively studied [11][12][13].…”

Section: Introductionmentioning

confidence: 99%

CASA derived human sperm abnormalities: correlation with chromatin packing and DNA fragmentation

Sivanarayana

Krishna

Prakash³

et al. 2012

J Assist Reprod Genet

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Purpose The present study was undertaken to evaluate the effects of morphokinetic abnormalities of human spermatozoa on chromatin packing and DNA integrity and possible beneficial effects of sperm selection in ICSI. Methods Semen samples from 1002 patients were analysed for morphology and motility using CASA. Protamine status and DNA fragmentation were analysed by chromomycin A3 staining and sperm chromatin dispersion assay respectively. Results Sperms with elongated, thin, round, pyri, amorphous, micro and macro forms were significantly higher in teratozoospermic and oligoasthenoteratozoospermic groups. Significant difference in chromatin packing and DNA fragmentation index was observed in these abnormal groups compared with normal. Similarly significant correlation was also seen between abnormal motility parameters and DNA fragmentation index in asthenozoospermic group compared with normal. Conclusions Specific abnormal morphological forms have higher incidence of chromatin packing abnormalities and DNA fragmentation. Using these sperms in ICSI might have an impact on fertilization, embryo development and abortion rates. These can be selectively avoided during ICSI procedure to improve ART outcome.

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“…É oportuno salientar que o corante AA é mais frequentemente utilizado para avaliações com citofotômetro de fluxo (Evenson et al, 1980, Sailer et al, 1996Ostermeier et al, 2001) e que, neste trabalho, os esfregaços corados com AA foram avaliados em microscopia de fluorescência (Tejada et al, 1984;Hamamah et al, 1990;Erenpreiss et al, 2001). O citofotômetro permite uma avaliação objetiva e rápida.…”

Section: Resultsunclassified

“…A cabeça dos espermatozóides dos mamíferos é quase que totalmente constituída de cromatina, portanto espera-se que alterações em tais estruturas sejam acompanhadas por anomalias morfológicas (Sailer et al, 1996;Ferrari et al, 1998;Ostermeier et al, 2001;Beletti et al, 2005).…”

Section: Introductionunclassified

Alterações de cromatina em espermatozóides de ovinos e caprinos avaliadas por azul de toluidina e alaranjado de acridina

2010

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RESUMOReprodutores com espermograma normal podem se comportar como subférteis ou passarem por períodos de subfertilidade. As alterações na cromatina dos espermatozóides são possíveis explicações encontradas para tais comportamentos. Conduziu-se este trabalho, com o objetivo de testar a eficiência de azul de toluidina (AT) e do alaranjado de acridina (AA) na identificação de alterações na compactação de cromatina em espermatozóides de ovinos e caprinos, além de avaliar a correlação entre essas alterações e as de morfologia espermática. Para tal, foram avaliadas amostras de sêmen de 15 ovinos e de 15 caprinos, com dez repetições para cada método por animal. Calcularam-se a média, o desvio padrão (DP) e o coeficiente de variação (CV) para cada técnica e animal. Utilizouse o teste t-Student para avaliar diferença entre as médias obtidas nos dois métodos. Também foram calculados a correlação de Pearson e os coeficientes kappa ponderado e não ponderado para avaliar a concordância entre os métodos com AT e AA. Foi verificado que nem sempre as anomalias morfológicas de cabeça são acompanhadas por alterações na cromatina identificáveis pelos métodos utilizados neste trabalho. O método AT é mais estável e possui maior sensibilidade do que AA para ambas as espécies, sendo o mais indicado para caprinos. Contudo, em razão de apresentar repetibilidade muito baixa, ambos os métodos não são indicados para avaliação espermática em ovinos.Termos para indexação: Azul de toluidina, alaranjado de acridina, espermatozóide, cromatina, DNA. ABSTRACTMales with normal spermogram can behave as subfertile or pass for periods of subfertility. Chromatin alterations of spermatozoa can account for such behavior. The objective of the present work was to test the efficacy of toluidine blue (TB) and acridine orange (AO) in the identification of alterations in chromatin compaction in spermatozoa from rams and goats, in addition to evaluate the correlation between those alterations and the ones of spermatic morphology. In order to do that, samples of semen from 15 rams and 15 goats were evaluated with 10 replications for each method. Mean, standard deviation and coefficient of variation were calculated for each animal and technique. Student's t-test was used to evaluate differences between the averages of two methods. Pearson correlation coefficient, weighted and non weighted kappa coefficient were also calculated to evaluate the agreement between methods with TB and AO. It was verified that morphological alterations of head not always are accompanied by alterations in chromatin identified by the methods used in this work. The TB method is more stable and sensitive than AO method for both species and this is most appropriate for goats. However, due to the very low repeatability, both methods are not indicated for evaluating ram spermatozoa.

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Bull sperm head morphometry related to abnormal chromatin structure and fertility

Cited by 14 publications

References 11 publications

Age-related changes in quality and fertility of porcine semen

Age-related changes in quality and fertility of porcine semen

CASA derived human sperm abnormalities: correlation with chromatin packing and DNA fragmentation

Alterações de cromatina em espermatozóides de ovinos e caprinos avaliadas por azul de toluidina e alaranjado de acridina

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