Bulk lysis procedures alter target cell population counts
Laura G. Rico,
Roser Salvia,
Michael D. Ward
et al.
Abstract:To achieve high‐sensitivity cell measurements (<1 in 105 cells) by flow cytometry (FCM), the minimum number of acquired cells must be considered and conventional immunophenotyping protocols fall short of these numbers. The bulk lysis (BL) assay is a standardized erythrocyte lysing approach that allows the analysis of the millions of cells required for high‐sensitivity measurable residual disease (MRD) detection. However, this approach has been associated with significant cell loss, along with potential over… Show more
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