Bulk Chemicals from Biotechnology: The Case of 1,3-Propanediol Production and the New Trends

Zeng, An‐Ping; Biebl, Hanno

doi:10.1007/3-540-45736-4_11

Cited by 266 publications

(259 citation statements)

References 49 publications

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“…Other cases involving anaerobic fermentation with glycerol as the sole carbon source have reported lower 1,3-PD concentrations of 70.4 g/liter (for Clostridium butyricum) and 70 to 78 g/liter (for Klebsiella pneumoniae) (14,22). Furthermore, the productivities and the conversion rates of glycerol to 1,3-PD in these systems were less than 1.57 g/liter/h and less than 75% (g/g), respectively (7,25,39). This work will be significant in exploring and finding a large-scale use for crude glycerol, which is the by-product of biodiesel, and will help lessen the requirements of growing supplies and give the biodiesel industry more revenue.…”

Section: Discussionmentioning

confidence: 79%

Microbial Conversion of Glycerol to 1,3-Propanediol by an Engineered Strain of Escherichia coli

Tang

Tan

Hong

et al. 2009

Appl Environ Microbiol

141

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In an effort to improve industrial production of 1,3-propanediol (1,3-PD), we engineered a novel polycistronic operon under the control of the temperature-sensitive lambda phage P L P R promoter regulated by the cIts857 repressor and expressed it in Escherichia coli K-12 ER2925. The genes for the production of 1,3-PD in Clostridium butyricum, dhaB1 and dhaB2, which encode the vitamin B 12 -independent glycerol dehydratase DhaB1 and its activating factor, DhaB2, respectively, were tandemly arrayed with the E. coli yqhD gene, which encodes the 1,3-propanediol oxidoreductase isoenzyme YqhD, an NADP-dependent dehydrogenase that can directly convert glycerol to 1,3-PD. The microbial conversion of 1,3-PD from glycerol by this recombinant E. coli strain was studied in a two-stage fermentation process. During the first stage, a novel high-cell-density fermentation step, there was significant cell growth and the majority of the metabolites produced were organic acids, mainly acetate. During the second stage, glycerol from the fresh medium was rapidly converted to 1,3-PD following a temperature shift from 30°C to 42°C. The by-products were mainly pyruvate and acetate. During this two-stage process, the overall 1,3-PD yield and productivity reached 104.4 g/liter and 2.61 g/liter/h, respectively, and the conversion rate of glycerol to 1,3-PD reached 90.2% (g/g). To our knowledge, this is the highest reported yield and productivity efficiency of 1,3-PD with glycerol as the sole source of carbon. Furthermore, the overall fermentation time was only 40 h, shorter than that of any other reports.

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Section: Discussionmentioning

confidence: 79%

Microbial Conversion of Glycerol to 1,3-Propanediol by an Engineered Strain of Escherichia coli

Tang

Tan

Hong

et al. 2009

Appl Environ Microbiol

141

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“…Then, pyruvate is converted to formic acid, lactic acid, 2,3-butanediol (via acetoin) and acetyl-CoA, that is oxidized into ethanol and acetic acid to generate NAD and ATP . Through the reductive pathway (b), glycerol is dehydrated to 3-hydroxypropionaldehyde by glycerol dehydratase (GDHt), and subsequently reduced to PDO by 1,3-propanediol dehydrogenase (PDO DH) (Zeng & Biebl, 2002). The catalytic activity of both enzymes depends on the presence of different co-factors: GDHt is coenzyme-B 12 dependent, while PDO DH is NADH+H + dependent (Kaur et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

1,3-Propanediol production from glycerol with a novel biocatalyst Shimwellia blattae ATCC 33430: Operational conditions and kinetics in batch cultivations

Rodríguez

Wojtusik

Ripoll

et al. 2016

Bioresource Technology

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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“…The liquid fraction of the whole stillage (i.e., thin stillage) contains a complex mixture of chemicals, including up to 2% glycerol [12]. In addition to the large amounts produced by the bioethanol industry [13,14], waste streams containing high levels of glycerol are generated in almost every industry that uses animal fats and vegetable oils as the starting material. For example, waste streams of 55-90% glycerol are readily generated by the oleochemical industry and this additional surplus may further drop glycerol prices [9].…”

mentioning

confidence: 99%

Anaerobic fermentation of glycerol: a platform for renewable fuels and chemicals

Clomburg

González

2013

Trends in Biotechnology

271

184

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Bulk Chemicals from Biotechnology: The Case of 1,3-Propanediol Production and the New Trends

Cited by 266 publications

References 49 publications

Microbial Conversion of Glycerol to 1,3-Propanediol by an Engineered Strain of Escherichia coli

Microbial Conversion of Glycerol to 1,3-Propanediol by an Engineered Strain of Escherichia coli

1,3-Propanediol production from glycerol with a novel biocatalyst Shimwellia blattae ATCC 33430: Operational conditions and kinetics in batch cultivations

Anaerobic fermentation of glycerol: a platform for renewable fuels and chemicals

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