Bulbus Fritillariae Cirrhosae as a Respiratory Medicine: Is There a Potential Drug in the Treatment of COVID-19?

Quan, Yunyun; Li, Li; Yin, Zhujun; Chen, Shilong; Yi, Jing; Lang, Jirui; Zhang, Lu; Yue, Qianhua; Zhao, Junning

doi:10.3389/fphar.2021.784335

Cited by 14 publications

(7 citation statements)

References 292 publications

(315 reference statements)

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“… 17 Its therapeutic effects are primarily mediated by biological processes, notably inflammation, to confer therapeutic benefits in lung diseases. 18 Currently, over 200 CBM-related products are available on the market. Examples include Nin Jiom Pei Pa Koa, Chuanbei Zhike Lu, and Chuanbei Pipa Capsules.…”

Section: Introductionmentioning

confidence: 99%

Network Pharmacology and Experimental Verification Reveal the Regulatory Mechanism of Chuanbeimu in Treating Chronic Obstructive Pulmonary Disease

Xian,

Xu,

Zheng

et al. 2024

COPD

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Background Chronic obstructive pulmonary disease (COPD) is a common respiratory disorder in pulmonology. Chuanbeimu (CBM) is a traditional Chinese medicinal herb for treating COPD and has been widely utilized in clinical practice. However, the mechanism of CBM in the treatment of COPD remains incompletely understood. This study aims to investigate the underlying therapeutic mechanism of CBM for COPD using network pharmacology and experimental approaches. Methods Active ingredients and their targets were obtained from the Traditional Chinese Medicine Systems Pharmacology database. COPD-associated targets were retrieved from the GeneCards database. The common targets for CBM and COPD were identified through Venn diagram analysis. Protein-protein interaction (PPI) networks and disease-herb-ingredient-target networks were constructed. Subsequently, the results of the network pharmacology were validated by molecular docking and in vitro experiments. Results Seven active ingredients and 32 potential targets for CBM were identified as closely associated with COPD. The results of the disease-herb-ingredient-target network and PPI network showed that peimisine emerged as the core ingredient, and SRC, ADRB2, MMP2 , and NOS3 were the potential targets for CBM in treating COPD. Molecular docking analysis confirmed that peimisine exhibited high binding affinity with SRC, ADRB2, MMP2 , and NOS3 . In vitro experiments demonstrated that peimisine significantly upregulated the expression of ADRB2 and NOS3 and downregulated the expression of SRC and MMP2 . Conclusion These findings indicate that CBM may modulate the expression of SRC, ADRB2, MMP2 , and NOS3 , thereby exerting a protective effect against COPD.

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Section: Introductionmentioning

confidence: 99%

Network Pharmacology and Experimental Verification Reveal the Regulatory Mechanism of Chuanbeimu in Treating Chronic Obstructive Pulmonary Disease

Xian,

Xu,

Zheng

et al. 2024

COPD

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“…Its ethnopharmacological use was first documented in Shennong Bencao Jing [ 1 ], the earliest classic text of Chinese materia medica in China compiled in the Eastern Han Dynasty (25–220 AD) [ 2 ]. Among various types of Fritillariae Bulbus, Fritillariae Cirrhosae Bulbus (FCB) is more valuable and regarded as “top grade” [ 3 ]. FCB has been officially documented as the bulbs of six Fritillaria species ( F. cirrhosa D.Don, F. unibracteata P.K.Hsiao & K.C.Hsia, F. przewalskii Maxim.…”

Section: Introductionmentioning

confidence: 99%

Determination of ITS1 haplotypes of Fritillariae Cirrhosae Bulbus by amplicon sequencing

Wu,

Wong,

Law

et al. 2024

Chin Med

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Background Fritillariae Cirrhosae Bulbus is an antitussive and expectorant Chinese medicinal material derived from the dried bulbs of six Fritillaria species. In the 2015 edition of the Chinese Pharmacopoeia, the polymerase chain reaction-restriction fragment length polymorphism (PCR–RFLP) is the officially listed method for their authenfication. Specifically, the ~ 300-bp ITS1 amplicon of only Fritillariae Cirrhosae Bulbus but not other Fritillaria species can be cleaved into two smaller fragments with restriction enzyme SmaI. Considering repeated reported cases of incomplete digestion of ITS1 amplicon, this study aims to investigate the possibility of heterogeneous ITS1 sequences contained in the Fritillariae Cirrhosae Bulbus. Methods In this study, ITS1 amplicons of Fritillaria Cirrhosae Bulbus and four other Fritillaria species were sequenced on Illumina platform. We utilised high-throughout amplicon sequencing to determine ITS1 haplotypes and their frequencies in Fritillaria genomes. Results Our results showed that all six botanical sources of Fritillariae Cirrhosae Bulbus indeed possess ITS1 haplotypes with no SmaI restriction site, and the average percentages of ITS1 reads containing SmaI restriction site ranged from 63.60% to 91.81%. Conclusion Our findings suggest that the incomplete digestion in PCR–RFLP analysis of Fritillariae Cirrhosae Bulbus is caused by the presence of ITS1 haplotypes without SmaI restriction site due to intragenomic heterogeneity.

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“…It is used as a medicine and healthcare material in many traditional Chinese medicine (TCM) prescriptions [2]. It has been used to treat chronic respiratory disorders such as asthma, cough, lung cancer, and tuberculosis [3]. Modern pharmacological studies have indicated that the bulb of F. cirrhosa contained a variety of bioactive isosteroidal alkaloids (ISA) such as imperialine, verticinone, verticine, delavine, peimisine, etc., which are mainly responsible for the anti-asthmatic, antioxidant, antitumor or anti-inflammatory action ascribed to them [4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis of CYP450 Family Members in Fritillaria cirrhosa D. Don and Profiling of Key CYP450s Related to Isosteroidal Alkaloid Biosynthesis

Xiao

et al. 2023

Genes

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Fritillaria cirrhosa D. Don (known as Chuan-Bei-Mu in Chinese) can synthesize isosteroidal alkaloids (ISA) with excellent medicinal value, and its bulb has become an indispensable ingredient in many patented drugs. Members of the cytochrome P450 (CYP450) gene superfamily have been shown to play essential roles in regulating steroidal alkaloids biosynthesis. However, little information is available on the P450s in F. cirrhosa. Here, we performed full-length transcriptome analysis and discovered 48 CYP450 genes belonging to 10 clans, 25 families, and 46 subfamilies. By combining phylogenetic trees, gene expression, and key F. cirrhosa ISA content analysis, we presumably identify seven FcCYP candidate genes, which may be hydroxylases active at the C-22, C-23, or C-26 positions in the late stages of ISA biosynthesis. The transcript expression levels of seven FcCYP candidate genes were positively correlated with the accumulation of three major alkaloids in bulbs of different ages. These data suggest that the candidate genes are most likely to be associated with ISA biosynthesis. Finally, the subcellular localization prediction of FcCYPs and transient expression analysis within Nicotiana benthamiana showed that the FcCYPs were mainly localized in the chloroplast. This study presents a systematic analysis of the CYP450 gene family in F. cirrhosa and provides a foundation for further functional characterization of the CYPs involved in ISA biosynthesis.

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Bulbus Fritillariae Cirrhosae as a Respiratory Medicine: Is There a Potential Drug in the Treatment of COVID-19?

Cited by 14 publications

References 292 publications

Network Pharmacology and Experimental Verification Reveal the Regulatory Mechanism of Chuanbeimu in Treating Chronic Obstructive Pulmonary Disease

Network Pharmacology and Experimental Verification Reveal the Regulatory Mechanism of Chuanbeimu in Treating Chronic Obstructive Pulmonary Disease

Determination of ITS1 haplotypes of Fritillariae Cirrhosae Bulbus by amplicon sequencing

Transcriptome Analysis of CYP450 Family Members in Fritillaria cirrhosa D. Don and Profiling of Key CYP450s Related to Isosteroidal Alkaloid Biosynthesis

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