Build‐up of Polypeptide Multilayer Coatings with Anti‐Inflammatory Properties Based on the Embedding of Piroxicam–Cyclodextrin Complexes

Abstract

We describe the build‐up of biomaterial coatings based on polypeptide multilayers possessing anti‐inflammatory properties. Poly(L‐lysine) (PLL) and poly(L‐glutamic acid) (PGA) are used as polypeptides, and piroxicam (Px) is used as the anti‐inflammatory agent. In order to embed high enough amounts of Px, the drug is incorporated in the films in the form of complexes with a charged 6A‐carboxymethylthio‐β‐cyclodextrin (cCD). It is shown that this cyclodextrin can solubilize higher amounts of Px than the cyclodextrins used commercially. The anti‐inflammatory properties are evaluated by determining the inhibition of TNFα production by human monocytic THP‐1 cells stimulated with lipopolysaccharide (LPS) bacterial endotoxin. Using Fourier‐transform (FT) Raman spectroscopy, we show that Px is mainly in the neutral form in cCD–Px complexes in solution, and that it remains biologically active under this form, whereas up to now only the zwitterionic form was reported to possess anti‐inflammatory properties. When incorporated in PLL/PGA multilayers, Px in the cCD–Px complexes changes from the neutral to the zwitterionic form. It is shown that these films present anti‐inflammatory properties, which can be delayed, and whose duration can be tuned by changing the film architecture.

UV degradation of deoxyribonucleic acid

“…A simple and versatile method for producing these architectures is the sequential build up of layers of functional materials by the layer-by-layer (LBL) technique [12,13]. This technique, initially applied to the production of polyelectrolyte thin films, has also been found to be suitable for the production of functionalized biomolecular architectures [14,15,16,17,18] and is therefore a relevant methodology for producing biological mimics to address radiation damage studies. However, it is fundamental to characterize the radiation degradation of the biological macromolecules in vacuum.…”

A living thick nanofibrous implant bifunctionalized with active growth factor and stem cells for bone regeneration

“…[6][7][8][9] Our strategy for regenerating bone combines a synthetic electrospun nanofibrous membrane (ENM) composed of the US Food and Drug Administration (FDA)-approved poly(ε-caprolactone) (PCL) polymer, and the bioactive growth factor BMP-2 entrapped into polymer nanoreservoirs built atop the nanofibers according to layer-by-layer (LbL) technology and fortified. [10][11][12][13][14][15][16][17][18][19][20][21] In bone repair, for a small lesion, we do not need bone substitute, as, at this early stage, the regenerative medicine is suitable for regenerating bone tissue. In regenerative medicine, the European and American authorities have already approved the use of BMP-2 or BMP-7 for bone regeneration applications.…”

“…13,15,16,19,21,[35][36][37][38][39][40][41][42] Polymer processing technologies such as electrospinning 43 allow nanofiber formation down to the 10 nm scale. Recently, we reported that it is possible to incorporate active growth factors (BMP-2, TGF-β1) as nanoreservoirs to induce bone formation.…”

Intratumoral Delivery of Paclitaxel in Solid Tumor from Biodegradable Hyaluronan Nanoparticle Formulations

“…Furthermore, most malignant solid tumors and their surrounding stromal tissue contain elevated levels of HA (9). CD44 is the principal cell surface receptor for HA, and it presents at low levels on epithelial, hemopoietic and some neuronal cells (11)(12)(13), and at elevated levels in various carcinoma, lymphoma, breast, colorectal, and lung tumor cells (14)(15)(16)(17). Overexpression of the HA receptors CD44 and RHAMM on cancer cells results in enhanced binding and endocytosis uptake of such compound.…”