Buffalo <i><scp>SREBP1</scp></i>: molecular cloning, expression and association analysis with milk production traits

Deng, Tingxian; Pang, Chaoyou; Ma, Xiaoya; Duan, Anqin; Liang, Shasha; Lu, Xingrong; Liang, Xianwei

doi:10.1111/age.12587

Cited by 9 publications

(7 citation statements)

References 6 publications

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“…The fold enrichment for BP, MF, and CC ranged from 3.61 to 9.81, 5.21 to 7.09, and 2.69 to 3.99, respectively. (Deng et al, 2016a) Intronic SNP (C/T at position 132437140) 2 PRL Milk yield, peak milk yield, and the contents of protein and fat in milk in Italian Mediterranean river buffalo (Li et al, 2017) 3 STAT5A Milk yield trait in the Binlangjiang buffalo (Ji et al, 2013) 3 SREBF1 305-d milk yield in crossbred Chinese buffaloes (Deng et al, 2017) 3 ACACA Expression patterns in mammary epithelial cells during lactation negatively correlated with milk yield in Murrah buffaloes (Yadav et al, 2015) Intronic SNP (G/A at position 49760383 and 49760609, respectively) 8 LEP High milk yield in Egyptian buffaloes (Nasr et al, 2016) 12 LIPIN1 Expression patterns in mammary epithelial cells during lactation positively correlated with milk yield in Murrah buffaloes (Yadav et al, 2015) 14 ACSS2 Expression patterns in mammary epithelial cells during lactation positively correlated with milk yield in Murrah buffaloes (Yadav et al, 2015) A 3'UTR ACSS2 SNP (G/A at position 19375891) 14 OXT Milk yield in Italian buffaloes (Pauciullo et al, 2012) 15 VPS13B Total milk yield, fat yield, and protein yield (Liu et al, 2018a) Intronic SNP (C/T at position 17737368)…”

Section: Resultsmentioning

confidence: 99%

Identification of candidate genes associated with milk yield trait in buffaloes ( Bubalus bubalis ) by restriction-site-associated DNA sequencing

et al. 2020

Revista Brasileira De Zootecnia

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Breeding and genetics Full-length research article Identification of candidate genes associated with milk yield trait in buffaloes (Bubalus bubalis) by restriction-site-associated DNA sequencing ABSTRACT-The objectives of our present study included the screening of single nucleotide polymorphisms (SNP) that show significant differences in allelic frequencies between two buffalo populations (Egyptian and Chinese buffaloes), categorization of functional genes associated with these SNP by gene ontology, and pathway analyses to further understand their potential values as candidate genes closely associated with milk yield trait in buffaloes. In this study, double digest restrictionsite associated DNA sequencing was performed on Illumina HiSeq 2500 platform for 20 and 25 female buffaloes from Egypt and China, respectively. Approximately 118 Gb of sequencing data were obtained, and a total of 110,129 and 150,535 putative SNP were detected in Egyptian and Chinese buffaloes, respectively. Focused only on those SNP that differed significantly in allelic frequencies between the two populations, we found that genes associated with these SNP were significantly over-represented in the ionotropic glutamate receptor pathway, the endothelin signaling pathway, and the gonadotropin-releasing hormone receptor pathway, which contained a total of 29 genes. Of these, nine genes (ADCY5, CACNA1A, CREB1, INHBA, INHBB, PIK3R1, PLCB1, PRKCE, and SMAD2) participating in the hormonal regulation of lactation, were considered to be promising candidate genes worthy of further investigations for favorable alleles associated with milk yield. Our results provide useful information about genetic variations in Egyptian and Chinese buffaloes. The potential influences of nine candidate genes and their associated SNP on milk yield need to be validated in more buffalo populations.

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Section: Resultsmentioning

confidence: 99%

Identification of candidate genes associated with milk yield trait in buffaloes ( Bubalus bubalis ) by restriction-site-associated DNA sequencing

et al. 2020

Revista Brasileira De Zootecnia

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“…ACTB was used as the reference gene for evaluation of relative gene expression in heart, liver, spleen, kidney and testis. And previous studies also used ACTB to determine gene expression in goat testis (Yao et al, 2014 ; Deng et al, 2017b ). The results were determined using the 2 −ΔΔCt method (Livak and Schmittgen, 2001 ).…”

Section: Methodsmentioning

confidence: 99%

Insertion/Deletion Within the KDM6A Gene Is Significantly Associated With Litter Size in Goat

et al. 2018

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A previous whole-genome association analysis identified lysine demethylase 6A (KDM6A), which encodes a type of histone demethylase, as a candidate gene associated to goat fecundity. KDM6A gene knockout mouse disrupts gametophyte development, suggesting that it has a critical role in reproduction. In this study, goat KDM6A mRNA expression profiles were determined, insertion/deletion (indel) variants in the gene identified, indel variants effect on KDM6A gene expression assessed, and their association with first-born litter size analyzed in 2326 healthy female Shaanbei white cashmere goats. KDM6A mRNA was expressed in all tissues tested (heart, liver, spleen, lung, kidney, muscle, brain, skin and testis); the expression levels in testes at different developmental stages [1-week-old (wk), 2, 3 wk, 1-month-old (mo), 1.5 and 2 mo] indicated a potential association with the mitosis-to-meiosis transition, implying that KDM6A may have an essential role in goat fertility. Meanwhile, two novel intronic indels of 16 bp and 5 bp were identified. Statistical analysis revealed that only the 16 bp indel was associated with first-born litter size (P < 0.01), and the average first-born litter size of individuals with an insertion/insertion genotype higher than that of those with the deletion/deletion genotype (P < 0.05). There was also a significant difference in genotype distributions of the 16 bp indel between mothers of single-lamb and multi-lamb litters in the studied goat population (P = 0.001). Consistently, the 16 bp indel also had a significant effect on KDM6A gene expression. Additionally, there was no significant linkage disequilibrium (LD) between these two indel loci, consistent with the association analysis results. Together, these findings suggest that the 16 bp indel in KDM6A may be useful for marker-assisted selection (MAS) of goats.

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“…A large number of studies have confirmed that SREBP1 gene plays a central role in the regulation of milk fat (Bionaz & Loor, ; Deng et al, ; Li et al, ; Maningat et al, ). Via construction of SREBP1 gene expression and the interference of the carrier, transfection cow mammary gland epithelial cells.…”

Section: Discussionmentioning

confidence: 96%

“…A large number of studies have confirmed that SREBP1 gene plays a central role in the regulation of milk fat (Bionaz & Loor, 2008;Deng et al, 2017;Maningat et al, 2009) Ma and Corl constructed a SREBP1 gene interference vector. After transfection of dairy cow mammary epithelial cells, the synthesis and absorption of fatty acid significantly reduced, and the expression level of ACACA, FAS, SCD, and FABP3 also reduced (Ma & Corl, 2012).…”

Section: Srebp1 Genes Regulate the Expression Of Related Genes In Mmentioning

confidence: 98%

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Isolation, characterization, and SREBP1 functional analysis of mammary epithelial cell inbuffalo

Chen

Jia

et al. 2019

J Food Biochem

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Compared to cow milk, buffalo milk contains more protein, fat, and vitamin. Buffalo milk is an ideal food in human life. Sterol regulatory element‐binding protein 1 (SREBP1), an important transcription factor, regulates the expression and activity of enzyme and protein involved in milk fat synthesis to influence on the synthesis and secretion of triglyceride in mammary epithelial cells. In the present study, we successfully isolated buffalo mammary epithelial cell by using enzymatic digestion, and then described the growth characteristics and expression characteristics of mammary epithelial cells. Moreover, we cloned the SREBP1 gene from total RNA isolated from milk fat globule and analyzed the function of the SREBP1 gene. After infected with shRNA‐SREBP1 lentiviral particle and treated with fatty acid, the expression trend of ACACA, FABP3, FAS, SCD, ERK1, ERK2, PPARy, and Insigl genes was consistent with the expression trend of SREBP1 gene. These results suggested that SREBP1 gene is a central transcription factor in regulating milk fat synthesis and SREBP1 gene may act on ERK1/ERK2 signaling pathway to regulate the expression of PPARy gene. The current study will provide a theoretical basis for further reveal the molecular mechanism of milk fat synthesis in buffalo mammary epithelial cells. Practical applications This study aim to separate and analysis characterization of mammary epithelial cell in buffalo. Compared to cow milk, buffalo milk contains more protein, fat, and vitamin. Buffalo milk is an ideal food in human life. This study will provide a theoretical basis for further research on the molecular mechanism of milk fat synthesis in buffalo mammary epithelial cells.

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Buffalo SREBP1: molecular cloning, expression and association analysis with milk production traits

Cited by 9 publications

References 6 publications

Identification of candidate genes associated with milk yield trait in buffaloes ( Bubalus bubalis ) by restriction-site-associated DNA sequencing

Identification of candidate genes associated with milk yield trait in buffaloes ( Bubalus bubalis ) by restriction-site-associated DNA sequencing

Insertion/Deletion Within the KDM6A Gene Is Significantly Associated With Litter Size in Goat

Isolation, characterization, and SREBP1 functional analysis of mammary epithelial cell inbuffalo

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