“…First, the more we use advanced technologies to dig into the transcription process, the more we uncover an unsuspected diversity in the features of transcriptomes, as highlighted by native elongating transcript sequencing (Xi et al, 2024), long-read sequencing (Koster et al, 2024), single-cell transcriptomics (Brettner et al, 2024;Nadal-Ribelles et al, 2024) or live imaging (Kindongo et al, 2024). Second, it is now obvious that transcription must be envisioned in a broader picture since it is tightly intertwined with other nuclear processes, that is, downstream events in RNA metabolism such as RNA processing (Albihlal et al, 2024;Struhl, 2023), localization and turnover, but also several DNAdependent transactions, including chromatin organization (Georis et al, 2023;Yague-Sanz, 2024), replication and repair (Piguet & Houseley, 2024). Finally, yeasts appear deliberately plural in the title of this Special Issue, not only in reference to the wide range of yeast models beyond S. cerevisiae and Schizosaccharomyces pombe, but also in view of their natural diversity, in-between isolates, or even within cell populations or colonies (Pangestu et al, 2023).…”