Budding of Frog Virus 3 Studied by Immunological and Cytochemical Methods in Electron Microscopy

Tripier, Françoise; Braunwald, J; Markovic, Ljubisa; Kirn, A.

doi:10.1159/000149768

Cited by 12 publications

(5 citation statements)

References 12 publications

(13 reference statements)

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“…In the present study, we were able to detect the presence of images compatible with the budding mechanism, which have not been previously observed in erythrocytic ICDV infections. No modifications of the budding-associated plasma membrane, such as the densifications associated with the Ranavirus buddings [44], could be detected in this study.…”

Section: Morphogenesiscontrasting

confidence: 66%

Experimental Infection of Lacertids with Lizard Erythrocytic Viruses

2002

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Objective: Lizard erythrocytic viruses (LEVs) produce inclusions in the cytoplasm of erythrocytes, but their impact on the infected host is poorly understood. This work reports on an experimental study of the infection process in Lacerta monticola and Lacerta schreiberi from Serra da Estrela Mountain, Portugal. Methods: A time sequence light microscope and transmission electron microscope (TEM) study of the infection process was performed in peripheral blood erythrocytes of experimentally infected lizards. Virions were searched for by TEM in visceral organs and bone marrow of the animals. Results: Infection was usually restricted to erythrocytes, but occasionally became systemic and induced disease. In the first case, a prevalence of infected erythrocytes of up to 98% followed by recovery was observed. In the latter, infection spread to leukocytes, leading to the death of the infected animals. Conclusions: The potential of LEVs to induce systemic infections was demonstrated. Sequential TEM examination of LEV-infected cells is described for the first time, demonstrating features such as dense inclusions related to virus nucleoid formation, intranuclear virions, intermediate structures in virion capsid morphogenesis and virus release by budding.

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Section: Morphogenesiscontrasting

confidence: 66%

Experimental Infection of Lacertids with Lizard Erythrocytic Viruses

2002

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“…Data from this study indicated that EHNV was similar to FV3 (Tripier et al 1974, Murti et al 1985a in that i t buds from both the elongated cellular processes which are rich in microfilaments (Murti et al 198513) and from thickened areas of cell membrane associated with the body of the cell. Cells infected with BIV, however, did not produce long cellular protrusions.…”

Section: Discussionsupporting

confidence: 54%

Comparison of epizootic haematopoietic necrosis and Bohle iridoviruses, recently isolated Australian iridoviruses

Hengstberger¹,

Hyatt²,

Speare³

et al. 1993

Dis. Aquat. Org.

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ABSTRACT. Epizootic haematopoletic necrosis virus (EHNV) and Bohle virus (BIV) are piscine and amphibian iridoviruses respectively which have been isolated in Australia from redfin perch Perca fluviatjlis L., cultured rainbow trout Oncorhynchus mykiss (Walbaum) and the ornate burrowing frog Limnodynastes ornatus (Gray). To determine whether the viruses were similar they were examined at the ultrastructural, protein, antigenic and genomic levels. EHNV was found to differ from BIV in cytopathic effect on host cells, size, site of virus release, molecular weights of proteins and restriction endonuclease digest profiles of the respective DNA's. On the other hand both iridoviruses \yere also found to infect a similar range of tissue culture cells, exhibit a similar sequence of ultrastuctural events and possess proteins of similar antigenic reactivity. DNA from EHNV and BIV cross hybridized to a high level. When the BIV polypeptides were compared to the published data for purified frog virus 3 (FV3) and with the appropriate restriction enzyme profiles, a similarity was noted between the 2 viruses. This implies that EHNV, which shares many structural and biochemical characteristics with BIV, is itself related to the Ranavirus genus of iridoviruses.

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“…The predominant polypeptides were ICPs 58 and 55, and the amount of ICP 58 was greatly enriched with respect to ICP 55 (a major structural virion polypeptide) compared with both whole cell extracts and purified FV3. It is possible that ICP 58 may be involved in modifying the plasma membrane, resulting in the changes of this membrane described by Tripier et al (76). This may enable the virus to acquire its external envelope.…”

Section: Discussionmentioning

confidence: 93%

“…The virus may leave the cell by budding through the plasma membrane, and modifications of the membrane occur at localized areas (16,42,77). The accumulation of virus-specific antigens at the plasma membrane has been detected by immunological and cytochemical methods involving electron microscopy (76).…”

mentioning

confidence: 99%

Frog Virus 3 Replication: Induction and Intracellular Distribution of Polypeptides in Infected Cells

Elliott

Kelly

1980

J Virol

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The synthesis of the polypeptides induced in frog virus 3-infected cells was analyzed by high-resolution sodium dodecyl sulfate-polyacrylamide gel electrophoresis of radiolabeled cell extracts. Purified frog virus 3 contained 22 polypeptides, with molecular weights in the range 9 x 103 to 114 x 103. All of the structural and an additional seven nonstructural polypeptides were detected in infected cell lysates. The following three classes of induced polypeptides (under temporal control) were observed in BHK cells: at 2 h, four a polypeptides; at 4 h, 13 18 polypeptides; and at 6 h, the remaining 12 y polypeptides. The total molecular weight of the infected cell-specific polypeptides (ICPs) was =1.5 x 106, which accounts for about 30% of the coding capacity of the viral genome. At least 10 of the induced polypeptides were phosphorylated, but none was glycosylated or sulfated. No evidence for posttranslation cleavage of polypeptides in pulse-chase and inhibition experiments was obtained. The synthesis of y polypeptides was not detected in the presence of the viral DNA replication inhibitors cytosine arabinoside and hydroxyurea, but halogenated nucleotides apparently had no effect. These results suggest that a and f polypeptides are "early" events and that detectable y polypeptide synthesis is dependent on the production of progeny viral DNA. The regulation of frog virus 3-induced polypeptide synthesis in infected BHK cells was examined by using inhibitors of protein and RNA synthesis and amino acid analogs. These experiments confirmed the existence of three sequentially synthesized, coordinately regulated classes of polypeptides, designated a, fl, and y. The requirements for the synthesis of each class were as

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Budding of Frog Virus 3 Studied by Immunological and Cytochemical Methods in Electron Microscopy

Cited by 12 publications

References 12 publications

Experimental Infection of Lacertids with Lizard Erythrocytic Viruses

Experimental Infection of Lacertids with Lizard Erythrocytic Viruses

Comparison of epizootic haematopoietic necrosis and Bohle iridoviruses, recently isolated Australian iridoviruses

Frog Virus 3 Replication: Induction and Intracellular Distribution of Polypeptides in Infected Cells

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