Budding Dynamics of Multicomponent Tubular Vesicles

Li, Li; Liang, Xinyu; Lin, Meiyu; Qiu, Feng; Yang, Yuliang

doi:10.1021/ja0567438

Cited by 36 publications

(55 citation statements)

References 15 publications

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“…[12][13][14] Experimentally, however, only a single work is known to the authors which reports on the dynamics of budded vesicles following a temperature jump. [10] In contrast to our work, the timescale of the observed process is of the order of seconds, the typical relaxation time of the system. Herein, we demonstrate that rapid heating of giant unilamellar lipid vesicles (GUVs) from their gel-to-fluid phase leads to the formation of dynamic, non-equilibrium buds.…”

Section: Introductionmentioning

confidence: 78%

“…[3,[4][5][6][7][8][9][10] Herein, however, the change in area takes place very rapidly, which leads to the formation of single buds of a diameter of about 1 mm during approximately 60 ms. Recalling that typical timescales of bulk and membrane diffusion are in the range of seconds on these size scales, [1][2][3][4] we expect dissipation to be a major contribution to the dynamics of the morphological change. A thorough theoretical model of the dynamics of the budding process is beyond the scope of this work.…”

Section: Dynamics Of Bud Formationmentioning

confidence: 98%

“…The subsequent relaxation into the global equilibrium shape takes place on the same timescale as reported earlier. [10] A theoretical approach following Sens [12] reveals that, while the number of Membrane budding has been extensively studied as an equilibrium process attributed to the formation of coexisting domains or changes in the vesicle area-to-volume ratio (reduced volume). In contrast, non-equilibrium budding remains experimentally widely unexplored, especially when timescales fall well below the characteristic diffusion time of lipids, t. We show that localized mechanical perturbations, initiated by driving giant unilamellar vesicles (GUVs) through their lipid main phase transition from the gel to the fluid phase, lead to the immediate formation of rapidly growing, localized, non-equilibrium buds when the transition takes place at short timescales (< t).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Phase‐Transition‐ and Dissipation‐Driven Budding in Lipid Vesicles

et al. 2009

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Membrane budding has been extensively studied as an equilibrium process attributed to the formation of coexisting domains or changes in the vesicle area-to-volume ratio (reduced volume). In contrast, non-equilibrium budding remains experimentally widely unexplored, especially when timescales fall well below the characteristic diffusion time of lipids, tau. We show that localized mechanical perturbations, initiated by driving giant unilamellar vesicles (GUVs) through their lipid main phase transition from the gel to the fluid phase, lead to the immediate formation of rapidly growing, localized, non-equilibrium buds when the transition takes place at short timescales (

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Section: Introductionmentioning

confidence: 78%

Section: Dynamics Of Bud Formationmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

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Phase‐Transition‐ and Dissipation‐Driven Budding in Lipid Vesicles

et al. 2009

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“…8 And the missing details from the previous study are provided now by AFM. Assuming that the selectivity of the antibody molecules is equal to all the fluorescence indicator molecules, it would be reasonable to draw the conclusion that the concentration of the fluorescence indicator molecules is higher at the boundary and lower inside the DOPC domains.…”

Section: Carbon Number Onmentioning

confidence: 99%

“…This process is studied in both optical microscope and atomic force microscope (AFM) by researchers. 6,7,8 However, the drawback of optical microscope is more and more obvious for the limited observation resolution, lack of details and unavoidable introduction of the fluorescence indicator. AFM study could provide us more detailed information compared with optical microscope with precisely vertical direction monitoring.…”

Section: Introductionmentioning

confidence: 99%

Atomic force microscopic study on lipid bilayer nanoscale phase separation

Li¹

2014

Bioinspired, Biomimetic and Nanobiomaterials

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Phase separation of copolymers or lipid membranes in nanoscale has attracted increasing interests for their applicationsin the synthesis of inorganic nanomaterial. The nanoscale phase separation of liquid bilayer as a supported membrane is systematically investigated by atomic force microscope (AFM). Moreover, the position of the fluorescence indicator, which is commonly used in the phase separation study in optical microscope, is also probed in this study to complete the knowledge of tradition microscopy research. Overall, the authors demonstrate the advantages of using AFM for phase separation study in this article. IntroductionLipids bilayer phase separation is always a popular research field as a simplest mimic model for microdomains or rafts in biosystems, which are believed to play a significant role in protein sorting, signal transducting and cell growth regulating.1,2,3 It also develops researchers interested in the nanomaterial research recently for the self-assembly domain formation in nanoscale, which could be potentially used as nanomaterials' template in nanomaterials bottom-up synthesis methods. 4,5 In the liquid crystal lipid domains, the soft hydrocarbon chain of the lipids generally is vertical to the surface of the membrane and their mobility is quite high, which allows their free movement inside of the membrane. In the solid state of the lipid domains, the rigid hydrocarbon chain would be vertical or shown a certain angle to the surface of the membrane. Typical phase separation occurs in the supported membrane formed with more than two lipid types. When the temperature is high enough, both types of liquids are soft and have high enough mobility in the membrane uniformly mixed together. With decreasing temperature, the mobility of the liquid is lowered until one of them achieved the solid phase. As a result, the phase separation is observed in normal cases. This process is studied in both optical microscope and atomic force microscope (AFM) by researchers. 6,7,8 However, the drawback of optical microscope is more and more obvious for the limited observation resolution, lack of details and unavoidable introduction of the fluorescence indicator. AFM study could provide us more detailed information compared with optical microscope with precisely vertical direction monitoring. In this article, the authors show two examples on the advantages of using AFM to study the lipid bilayer phase separation. The first part shows that difference in the same lipid bilayers, 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC)/sphingomyelin (SM), when different SM sources are introduced. Although no difference would be observed from the optical microscope, the AFM could easily find out the difference. In the second case, the commonly used fluorescence indicator, Texas Red, is introduced in the films. The precise position dispersion of the fluorescence indicator is also probed in AFM study. Experimental section Supported membrane synthesisThe lipids used in this study are DOPC, SM from cow brain (BSM) and SM from egg ...

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Giant Vesicles: Preparations and Applications

et al. 2010

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There is considerable interest in preparing cell-sized giant unilamellar vesicles from natural or nonnatural amphiphiles because a giant vesicle membrane resembles the self-closed lipid matrix of the plasma membrane of all biological cells. Currently, giant vesicles are applied to investigate certain aspects of biomembranes. Examples include lateral lipid heterogeneities, membrane budding and fission, activities of reconstituted membrane proteins, or membrane permeabilization caused by added chemical compounds. One of the challenging applications of giant vesicles include gene expressions inside the vesicles with the ultimate goal of constructing a dynamic artificial cell-like system that is endowed with all those essential features of living cells that distinguish them from the nonliving form of matter. Although this goal still seems to be far away and currently difficult to reach, it is expected that progress in this and other fields of giant vesicle research strongly depend on whether reliable methods for the reproducible preparation of giant vesicles are available. The key concepts of currently known methods for preparing giant unilamellar vesicles are summarized, and advantages and disadvantages of the main methods are compared and critically discussed.

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Budding Dynamics of Multicomponent Tubular Vesicles

Cited by 36 publications

References 15 publications

Phase‐Transition‐ and Dissipation‐Driven Budding in Lipid Vesicles

Phase‐Transition‐ and Dissipation‐Driven Budding in Lipid Vesicles

Atomic force microscopic study on lipid bilayer nanoscale phase separation

Giant Vesicles: Preparations and Applications

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