Buccal swabs as a reliable source of DNA for sexing young and adult Common Swifts (Apus apus)

Wellbrock, Arndt H. J.; Bauch, Christina; Rozman, Jan; Witte, Klaudia

doi:10.1007/s10336-012-0843-1

Cited by 19 publications

(16 citation statements)

References 14 publications

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“…Sex determination was 100% correct as validated by sexually dimorphic plumage. The success rate reported here is markedly higher than the rate reported by two previous studies 7,9 . The DNA extraction with Chelex minimizes pipetting and precipitation steps in which material can be lost.…”

Section: Discussioncontrasting

confidence: 81%

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Who is Who? Non-invasive Methods to Individually Sex and Mark Altricial Chicks

Adam

Scharff

Honarmand

2014

JoVE

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Many experiments require early determination of offspring's sex as well as early marking of newborns for individual recognition. According to animal welfare guidelines, non-invasive techniques should be preferred whenever applicable. In our group, we work on different species of song birds in the lab and in the field, and we successfully apply non-invasive methods to sex and individually mark chicks. This paper presents a comprehensive non-invasive tool-box. Sexing birds prior to the expression of secondary sexual traits requires the collection of DNA-bearing material for PCR. We established a quick and easy method to sex birds of any age (post hatching) by extracting DNA from buccal swabs. Results can be obtained within 3 hours. For individual marking chick's down feathers are trimmed in specific patterns allowing fast identification within the hatching order. This set of methods is easily applicable in a standard equipped lab and especially suitable for working in the field as no special equipment is required for sampling and storage. Handling of chicks is minimized and marking and sexing techniques are non-invasive thereby supporting the RRR-principle of animal welfare guidelines.

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Section: Discussioncontrasting

confidence: 81%

“…In birds, DNA sexing can be performed on a number of non-invasively obtainable materials as droppings 2 , feathers 3,4 or buccal swabs 3,5,9 . Regardless of subject´s condition and age buccal swabs are the method of choice for avian sexing, because they are easy to perform, rarely fail and handling is short.…”

Section: Introductionmentioning

confidence: 99%

Who is Who? Non-invasive Methods to Individually Sex and Mark Altricial Chicks

Adam

Scharff

Honarmand

2014

JoVE

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“…First, animal welfare considerations should prompt researchers to renew their effort to improve current CHMS procedures (Cazaux, 2007). A variety of methods for capture, blood sampling, marking, tagging and tracking are available with recent novelties and ongoing technological progress (Bonter & Bridge, 2011;Fair et al, 2010;Murray & Fuller, 2000;Owen, 2011;Watson, 2012;Wellbrock, Bauch, Rozman, & Witte, 2012).…”

Section: Methodological Implicationsmentioning

confidence: 98%

Immediate effects of capture on nest visits of breeding blue tits, Cyanistes caeruleus, are substantial

Schlicht

Kempenaers

2015

Animal Behaviour

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“…Buccal swabs are now regularly used for population genetics in a number of species: amphibians (Pidancier et al 2003;Broquet et al 2007a;Gallardo et al 2012), fish (Reid et al 2012) and mammals (Corthals et al 2015). Buccal swabs have lately been used in bird studies (e.g., Bush et al 2005;Handel et al 2006;Brubaker et al 2011;Yannic et al 2011), and a few studies have demonstrated the reliability of this sampling method for bird sex identification (Arima and Ohnishi 2006;Handel et al 2006;Wellbrock et al 2012;Dawson et al 2015). Finally, a strictly noninvasive approach would be to sex birds using DNA extracted from shed feathers.…”

Section: Introductionmentioning

confidence: 99%

Genetic and morphological sex identification methods reveal a male-biased sex ratio in the Ivory Gull Pagophila eburnea

et al. 2016

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International audienceSex identification of birds is relevant to studies of evolutionary biology and ecology and is often a central issue for the management and conservation of populations. The Ivory Gull Pagophila eburnea (Phipps, 1774) is a rare high-Arctic species whose main habitat is sea ice throughout the year. This species is currently listed Near Threatened by the IUCN, because populations have drastically declined in part of the species distribution in the recent past. Here we tested molecular sexing methods with different types of samples. Molecular sexing appeared to be very efficient with DNA extracted from muscle, blood, and buccal swabs, both for adults and young chicks. We also performed morphological analyses to characterize sexual size dimorphism in Ivory Gulls sampled in three distinct regions: Greenland, Svalbard, and Russia. Males were larger than females for all morphometric measurements, with little overlap between sexes. Discriminant analysis based on six morphometric variables correctly classified ~95 % of the individuals, even when using two variables only, i.e., gonys height and skull length. Therefore, both molecular and biometric methods are useful for sexing Ivory Gulls. Interestingly, our results indicate a male-biased sex ratio across all Ivory Gull populations studied, including two samples of offspring (67.8 % males)

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Buccal swabs as a reliable source of DNA for sexing young and adult Common Swifts (Apus apus)

Cited by 19 publications

References 14 publications

Who is Who? Non-invasive Methods to Individually Sex and Mark Altricial Chicks

Who is Who? Non-invasive Methods to Individually Sex and Mark Altricial Chicks

Immediate effects of capture on nest visits of breeding blue tits, Cyanistes caeruleus, are substantial

Genetic and morphological sex identification methods reveal a male-biased sex ratio in the Ivory Gull Pagophila eburnea

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