Buccal Pouch of the Syrian Hamster (Cricetus auratus) : Importance in Experimental Oral Biology

Gardner, Alvin F.

doi:10.1177/00220345640430063201

Cited by 7 publications

(4 citation statements)

References 12 publications

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“…A review of the literature shows there are few CK-isoenzyme studies in oral malignancies, particularly in animal model. The normal hamster buccal pouch tissue (HBPT) is composed of a thin squamous cell epithelial layer, connective tissue and a muscle layer and is one of the most commonly used animal models for oral carcinogenesis (29). The purpose of this study was to determine the sequential comparative changes in CK isoenzyme activity in serum and HBPT during DMBA induced carcinogenesis.…”

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confidence: 99%

Creatine kinase isoenzymes activity in serum and buccal pouch tissue of hamsters during DMBA‐induced squamous cell carcinogenesis

Liu

Chen

1991

J Oral Pathology Medicine

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The sequential changes of creatine kinase (CK) isoenzyme activity in serum and hamster buccal pouch tissue (HBPT) of normal, premalignant, and malignant stages during 13 wk of DMBA induced hamster buccal pouch squamous cell carcinogenesis, were studied. We found that the total CK activity in both serum and HBPT of the DMBA painted groups increased during the premalignant period (3-7 wk), with the peak being at 7 wk, while declining in the malignant period (9-13 wk). No significant difference in the serum and HBPT CK-MM, CK-MB and CK-BB activity, in DMBA-induced carcinogenesis, could be established. However, the macro CK type 2 (MCK-2), a cathodically migrating atypical creatine kinase isoenzyme, initially appeared in the sera and tissues of the 3rd wk DMBA-treated group. Its activity gradually increased during the premalignant and malignant stages, paralleling the increasing number of DMBA applications. There was a correlation between the degree of differentiation, and the size and number of the induced tumors with MCK-2. No MCK-2 could be detected in any of the sera or tissues of the control, nor in the 1st wk DMBA-treated group. We propose that MCK-2 is a potential tumor marker in oral malignancy and its presence may provide valuable information for early diagnosis of a possibly premalignant transforming oral lesion.

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confidence: 99%

Creatine kinase isoenzymes activity in serum and buccal pouch tissue of hamsters during DMBA‐induced squamous cell carcinogenesis

Liu

Chen

1991

J Oral Pathology Medicine

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“…The mucosa of the hamster buccal pouch is a squamous epithelium composed of three to four cell layers. This epithelium is one of the most commonly used models for oral carcinogenesis (17). The histology of such experimentally induced squamous cell carcinoma has been studied in detail, and histochemical techniques have been used to localize enzymes in such tumors (18,19).…”

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confidence: 99%

Lectin binding patterns in squamous epithelium in experimentally induced hamster buccal pouch carcinoma

Jin

Lin

1989

J Oral Pathology Medicine

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The distribution pattern of certain monosaccharides in the epithelial cells of the hamster buccal pouch was studied during carcinoma development induced by 9,10-dimethyl-1,2-benzathrancene (DMBA). An avidin-biotin-peroxidase complex (ABC) immunohistochemical technique with high affinity biotinylated lectins was employed to identify monosaccharides. Lectins used in this experiment included Concanavalin A (Con A), for identifying mannose or glucose, Ricinus communis agglutinin I(RCA-I), for identifying galactose, and Ulex europaeus agglutinin I(UEA-I), for identifying fucose. The results show that in normal buccal pouch epithelial cells, fuctose or galactose were concentrated predominantly on the cellular membrane, while mannose and glucose were distributed in the cytoplasm. In the epithelial cells undergoing neoplastic transformation induced by DMBA, most cells showed decreased staining of the above-mentioned monosaccharides, while in other areas the cells were heavily stained. However, the most striking change which occurred was that galactose and fucose shifted from the cellular membrane to the intracytoplasmic area during the malignant transformation. Thus, the changes of anatomic location and intensity of staining of monosaccharides in the buccal pouch epithelium may be used as a criteria for early histochemical diagnosis of malignant transformation.

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“…On the other hand, embryologically. these epithelia originate from a different germ layer: the ectoderm gives rise to the cheek pouch epi thelium [Gardner, 1964]. whereas the endodenn gives rise to the esophageal epithelium.…”

Section: Discussionmentioning

confidence: 99%

“…1991: Veys et al" 1994. Moreover, it long represents a suitable animal model system in experiments inducing squamous-cell car cinogenesis [Gardner, 1964;Evenson, 1981). This model has shed light on the formation of human squamous carci-growth factors and oncogenes |Wong et al.…”

Section: Introductionmentioning

confidence: 99%

Differential Expression of Cytokeratin mRNA in Hamster Squamous Epithelia as Observed by in situ Hybridization Using Human Cytokeratin cRNA Probes

Baert

Viaene²,

Veys³

1995

Cells Tissues Organs

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In this hybridohistochemical study, we investigated the expression of cytokeratin (CK) mRNA in the keratinizing squamous epithelium of hamster cheek pouch and esophagus, using eight different digoxigenin-labelled RNA probes complementary to human CK mRNAs. CK 4, CK 6, CK 8, CK 14, and CK 15 RNA probe obviously hybridized with hamster counterpart mRNA(s) in the cheek pouch as well as in the esophageal epithelium. However, using the CK 10-specific RNA probe, only the cheek pouch epithelium exhibited a positive reaction. We were not able to detect any positive signal for the CK 18 or for the CK 19 RNA probe. We observed three different CK mRNA distribution patterns in the cheek pouch epithelium, and four in the esophageal epithelium. The differences in expression and distribution pattern of CK mRNAs between the two types of epithelia suggest that the hamster CK polypeptide family comprises at least six different species. We also conclude that human cRNA probes for CK mRNAs may provide a way to detect changes in CK expression and distribution during induced non-neoplastic and neoplastic changes in the hamster cheek pouch model. This may also help to elucidate the molecular pathogenesis of squamous-cell carcinoma.

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Buccal Pouch of the Syrian Hamster (Cricetus auratus) : Importance in Experimental Oral Biology

Cited by 7 publications

References 12 publications

Creatine kinase isoenzymes activity in serum and buccal pouch tissue of hamsters during DMBA‐induced squamous cell carcinogenesis

Creatine kinase isoenzymes activity in serum and buccal pouch tissue of hamsters during DMBA‐induced squamous cell carcinogenesis

Lectin binding patterns in squamous epithelium in experimentally induced hamster buccal pouch carcinoma

Differential Expression of Cytokeratin mRNA in Hamster Squamous Epithelia as Observed by in situ Hybridization Using Human Cytokeratin cRNA Probes

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