Buccal epithelial cell micronuclei: Sensitive, non-invasive biomarkers of occupational exposure to low doses of ionizing radiation

Torres, Laura Aguiar; Rodrigues, Armindo; Linhares, Diana; Camarinho, Ricardo; Rego, Zelia; Garcia, Patrícia

doi:10.1016/j.mrgentox.2018.12.009

Cited by 11 publications

(11 citation statements)

References 33 publications

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“…The micronucleus average found in this group was consistent with values found in other studies, such as Bonassi et al (2011) and Holland et al (2008). Moreover, the micronucleus averages found in the study by da Silva Júnior et al (2020) are even less than the values reported by Holland et al (2008) in subjects exposed to ionizing radiation, and with values closer to those reported by Aguiar Torres et al (2019) among workers exposed to low doses of ionizing radiation.…”

supporting

confidence: 91%

Response to letter to the editor “Genetic biomonitoring of professionals occupationally exposed to ionizing radiation: Theoretical concepts for scientific debate”

et al. 2020

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We would like to thank the journal for the opportunity to respond to the issues raised in the letter to the editor "Genetic biomonitoring of professionals occupationally exposed to ionizing radiation: Theoretical concepts for scientific debate" and to clarify aspects of our methodology in relation to these concerns. We would also like to thank the letter's authors for their interest in our work and for taking the time to express their valuable points of view. First, in their letter to the editor, the authors raise concerns about the staining procedure used. In this regard, we agree that the use of stains like Giemsa in oral mucosa cells has been questioned by researchers like Bonassi et al. (2011) (the study referenced by the letter's authors); however, this study does not bring comparative results with the use of eosin methylene blue according to Leishman. In fact, the same study does not rule out the use of simple staining techniques, such as May-Grünwald. Even so, recent studies, as mentioned by Antoniazzi et al. (2018) and other studies (Vilas Boas et al., 2018), showed promising and reliable results with the Giemsa staining technique. The staining procedure used in the study by da Silva Júnior et al. (2020), a solution of eosin methylene blue according to Leishman, is an appropriate cocktail for differential visualization of both cytoplasm and cell nucleus. Korsakov et al. (2015) used the solution of eosin methylene blue according to Leishman for staining oral cells in their study that recommended the use of oral epithelium cells as an environmental indicator.

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supporting

confidence: 91%

Response to letter to the editor “Genetic biomonitoring of professionals occupationally exposed to ionizing radiation: Theoretical concepts for scientific debate”

et al. 2020

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“…It is the only method capable to show genotoxic effects of moderate concentrations of radon, as those that are met in unventilated rooms, basements or caves [14,97]. Moreover, the sensitivity of BMn allowed also to show genotoxic effects of work in healthcare, where staff was exposed to very low doses of radiation [98]. BMn is gaining popularity and probably will become a standard cytogenetic test, especially since it is minimally invasive, easy to perform, and cell samples are taken from the oral cavity.…”

Section: Buccal Mn (Bmm)-mature But Underused Assaymentioning

confidence: 99%

Micronucleus Assay: The State of Art, and Future Directions

Sommer

Buraczewska

Kruszewski

2020

IJMS

164

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During almost 40 years of use, the micronucleus assay (MN) has become one of the most popular methods to assess genotoxicity of different chemical and physical factors, including ionizing radiation-induced DNA damage. In this minireview, we focus on the position of MN among the other genotoxicity tests, its usefulness in different applications and visibility by international organizations, such as International Atomic Energy Agency, Organization for Economic Co-operation and Development and International Organization for Standardization. In addition, the mechanism of micronuclei formation is discussed. Finally, foreseen directions of the MN development are pointed, such as automation, buccal cells MN and chromothripsis phenomenon.

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“…Radiation detectors such as Geiger-Muller counters, scintillation counters, personal dosimeters, and personal protective equipment should be available in emergency departments. In addition, the whole emergency depart-ment staff should be trained to wear the PPE properly (6,22,23). The amount of radiation received by the person is quickly measured with detectors and treatment is prioritized according to the amount of radiation received.…”

Section: Discussionmentioning

confidence: 99%

Emergency Department Preparedness of Hospitals for Radiation, Nuclear Accidents, and Nuclear Terrorism: A Qualitative Study in Iran

Marzaleh

Rezaee

Rezaianzadeh

et al. 2019

Iran Red Crescent Med J

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Background:The emergency department is the entrance gate of patients to a hospital. Hospitals are confronted with major challenges in radiation, nuclear accidents, and nuclear terrorism. Iran is also at risk of disasters, accidents, and threats, so, the possibility of nuclear and radiation accidents cannot be neglected. Objectives: The present study aimed to extract the effective factors in emergency department preparedness of hospitals for radiation, nuclear accidents, and nuclear terrorism in Iran. Methods: This qualitative study was conducted using in-depth semi-structured interviews with 32 key informants selected through purposive and snowball sampling. Experts were from seven different specialties. Data were analyzed using the thematic analysis method in order to extract the effective factors in emergency department preparedness of hospitals for radiation, nuclear accidents, and nuclear terrorism in Iran in 2019. The interviews were held in the cities of Bushehr, Tehran, Shiraz, and Isfahan from September 2018 to February 2019. Results: Effective factors in emergency department preparedness of hospitals were categorized into staff preparedness, equipment preparedness, and system preparedness with 20 subcategories. The experts emphasized that training courses and exercises could enhance the preparedness and response to these accidents. Conclusions: This study showed that the emergency departments of hospitals in Iran have many challenges. When the country moves towards having nuclear technology, must also provide the infrastructure of the preparedness. There must be an attempt to reduce these challenges by providing financial and structural support. Identifying effective factors in preparation can be helpful in setting up programs for emergency department preparedness of hospitals against nuclear and radiation accidents.

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Buccal epithelial cell micronuclei: Sensitive, non-invasive biomarkers of occupational exposure to low doses of ionizing radiation

Cited by 11 publications

References 33 publications

Response to letter to the editor “Genetic biomonitoring of professionals occupationally exposed to ionizing radiation: Theoretical concepts for scientific debate”

Response to letter to the editor “Genetic biomonitoring of professionals occupationally exposed to ionizing radiation: Theoretical concepts for scientific debate”

Micronucleus Assay: The State of Art, and Future Directions

Emergency Department Preparedness of Hospitals for Radiation, Nuclear Accidents, and Nuclear Terrorism: A Qualitative Study in Iran

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