Buccal Cell DNA Extraction: Yield, Purity, and Cost: A Comparison of Two Methods

Saab, Yolande B.; Kabbara, Wissam K; Chbib, Christiane; Gard, Paul R.

doi:10.1089/gte.2007.0044

Cited by 21 publications

(16 citation statements)

References 11 publications

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“…However, 2,782 (21.3%) of the 13,084 subjects who consented failed to return appropriate samples, and 3,968 (30.3%) did not yield appropriate DNA on the first attempt. In addition, although the A260/280 ratio may not be an accurate measure of nucleic acid purity and quality can be better assessed by attempting PCR amplification [4,31], low A260/280 ratio (<1.8) may suggest that buccal swab samples are subject to contamination. This finding suggests that although the buccal cell swab procedure is simple and easy, there is a degree of inter-individual variation in the amount and quality of extracted DNA because it depends on characteristics of the buccal mucosa and the force exercised with the swab.…”

Section: Discussionmentioning

confidence: 99%

Factors Associated with Participation in Providing Buccal Cell DNA for a Genetic Epidemiologic Study

Kim

Hur

et al. 2010

Public Health Genomics

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Aims: Our aim was to investigate factors associated with participation in buccal cell DNA collection using a large-scale prospective cohort study. Methods: Of the 183,634 Korea Medical Insurance Corporation (KMIC) prospective cohort study enrollees, 44,773 (36,794 men) were randomly selected. Buccal cell collection kits were mailed and collected from March to September 2004. Epidemiologic characteristics measured at baseline (1990 or 1992) and during follow-up (1998) were compared between respondents and nonrespondents. Results: Among the 13,084 (29.2%) respondents, 78.7% submitted usable samples (DNA concentration ≧3 µg). After adjustments for either or both age and family history of chronic disease, participation in men was positively associated with old age, a family history of chronic disease, low subjective health status, and regular exercise, and negatively associated with current smoking status. In women, only old age was associated with participation. Low body mass index (BMI) (<18.5 kg/m²), blood pressure, total cholesterol, and fasting blood glucose level were negatively associated with participation in men after adjustments for age and family history of chronic disease, but not in women, except for low BMI. Conclusions: In this study, collecting buccal cell DNA by mailed survey has a low success rate (29%), and there appear to be a nonresponse bias and gender differences in the collection process.

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Section: Discussionmentioning

confidence: 99%

Factors Associated with Participation in Providing Buccal Cell DNA for a Genetic Epidemiologic Study

Kim

Hur

et al. 2010

Public Health Genomics

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“…However, other simple and cost-effective methods can be used with the similar results [5]. Compared with other sources of DNA, such as blood or hair, this method does not require the use of specialized consumables (syringes, anticoagulant-treated tubes, etc).…”

Section: Dna Purificationmentioning

confidence: 97%

Biochemistry and molecular biology techniques for person characterization

Herrero¹,

Ivorra²,

Garcia-Sogo³

et al. 2008

Biochem Molecular Bio Educ

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Using the traditional serological tests and the most novel techniques for DNA fingerprinting, forensic scientists scan different traits that vary from person to person and use the data to include or exclude suspects based on matching with the evidence obtained in a criminal case. Although the forensic application of these methods is well known, the procedures and techniques used to obtain these results are not so well studied. Here, we report a laboratory exercise aimed to familiarize the students in several of the methods and markers employed for people individualization. With this exercise, students have to characterize themselves and an unknown sample according to the following characteristics: ABO blood group, presence or absence of Y chromosome, and their polymorphism for a variable number of tandem repeats (VNTR). This exercise has been originally developed for students of the course ''Techniques in Criminology'' within the Bachelor of Criminology. Although the schedule and procedures have been addressed to students who are faced for their first time with a laboratory of molecular biology, the exercise can also be adapted to students in more specialized courses and studies.

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“…The subjects abstained from smoking, drinking, and eating for 2 hours before buccal swab collection. DNA was extracted from the buccal cells after period of storage at 4°C, using a protocol from Saab et al (22). Then the tubes were heated at 95 °C for 7 min, after which the swab was carefully removed, discarded and treated with 30 μL of Tris-HCl (1 M; pH 8.0).…”

Section: Participantsmentioning

confidence: 99%

Genetic Variants and Mixed Sport Disciplines: A Comparison among Soccer, Combat and Motorcycle Athletes

Cocci

Pistolesi

Guercioni

et al. 2019

Ann. Appl. Sport Sci

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Background. Soccer, combat sports and motorcycling have all been classified as mixed in terms of their metabolism predominance thus showing a common multifaceted nature of physical requirements, which include aerobic capacity, strength, endurance, power and agility. Objectives. The aim of the current study was to compare allelic and genotype frequencies of four well-known polymorphisms among athletes from different mixed sport disciplines in order to investigate genetic markers suitable for distinguishing the predominant components of these sports. Methods. Genotyping for the angiotensin-converting enzyme (ACE) insertion/deletion (I/D), α-actinin-3 (ACTN3) R577X, muscle-specific creatine kinase (CK-MM) A/G and peroxisome proliferator-activated receptor alpha (PPARα) G/C polymorphisms among 113 professional athletes from motorcycling, soccer and combat sports was performed by PCR-RFLP method. Results. We found that the G allele of the PPARα polymorphism was significantly (p<0.05) more frequent in soccer players than in combat sport athletes or motorcycle riders. In addition, we observed that the genotype frequency of AA of CK-MM (rs8111989) polymorphism in soccer players was significantly (p<0.05) higher than combat athletes. On the contrary, both ACE I/D and ACTN3 R577X polymorphisms do not enable to distinguish between these disciplines. Conclusions. Our findings indicate that the PPARα polymorphism may be suitable as potential distinguish genetic marker among mixed sport disciplines.

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Buccal Cell DNA Extraction: Yield, Purity, and Cost: A Comparison of Two Methods

Cited by 21 publications

References 11 publications

Factors Associated with Participation in Providing Buccal Cell DNA for a Genetic Epidemiologic Study

Factors Associated with Participation in Providing Buccal Cell DNA for a Genetic Epidemiologic Study

Biochemistry and molecular biology techniques for person characterization

Genetic Variants and Mixed Sport Disciplines: A Comparison among Soccer, Combat and Motorcycle Athletes

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