BubR1 is a spindle assembly checkpoint protein regulating meiotic cell cycle progression of mouse oocyte

Sirt2‐BubR1 acetylation pathway mediates the effects of advanced maternal age on oocyte quality

“…Similarly, a marked reduction of BubR1 levels was detected in oocytes from old women and aged mice (Lagirand‐Cantaloube et al., 2017; Pan, Ma, Zhu, & Schultz, 2008; Riris et al., 2014). Moreover, increasing evidence suggests that BubR1 insufficiency is closely associated with high frequency of spindle/chromosome defects and resultant aneuploidy in oocytes (Baker et al., 2004; Wei et al., 2010). Of note, BubR1 was identified to be acetylated at K250 at prometaphase in human cells.…”

“…Through SAC signal activation, BubR1 participates in proper chromosome segregation during mitosis (Baker et al., 2013; Lampson & Kapoor, 2005). BubR1, is required at several key steps in oocyte meiosis, and more specifically, for SAC activity, the timing of the first meiotic division, and the stable attachment of chromosomes to the spindle (Brunet, Pahlavan, Taylor, & Maro, 2003; Wei et al., 2010). In oocytes, BubR1 is required for the establishment of robust K‐MT attachments in a meiosis‐specific manner (Wei et al., 2010).…”

“…BubR1, is required at several key steps in oocyte meiosis, and more specifically, for SAC activity, the timing of the first meiotic division, and the stable attachment of chromosomes to the spindle (Brunet, Pahlavan, Taylor, & Maro, 2003; Wei et al., 2010). In oocytes, BubR1 is required for the establishment of robust K‐MT attachments in a meiosis‐specific manner (Wei et al., 2010). It is worth noting that a marked reduction of BubR1 levels was detected in aged oocytes from both human and mice (Baker et al., 2004; Riris, Webster, & Homer, 2014).…”

Nuf2 is required for chromosome segregation during mouse oocyte meiotic maturation

“…Several studies revealed that SAC protein such as mitotic arrest-defiient-1 (Mad1), Mad2, Budding uninhibited by benzimidazole-1 (Bub1), Bub3, BubR1 are all involved in cell cycle progression. 5,7,8 The results prompted us to explore whether overexpressed Nuf2 could regulate the spindle assembly checkpoint in meiosis after Nuf2 overexpression. Precise signals for Bub3 were detected in MI-arrested oocytes in the overexpressed group after 10 h of culture, while the control oocytes entered anaphase.…”

“…3,4 SAC surveys the interactions between chromosomes and microtubules, and will delay cell cycle timing to allow additional time for corrections. [5][6][7][8] The Ndc80 complex has been identified as a bridge of kinetochore-microtubule attachment and a necessary factor for SAC regulation. [9][10][11][12][13][14] Nuf2 is the core component of the Ndc80 complex, 15,16 and the complex is a heterotetramer of Ndc80 (also called Hec1 in humans 15 ), Nuf2, Spc24 and Spc25.…”