BTK mediated apoptosis, a possible mechanism for failure to generate high titer retroviral producer clones

A novel reverse transduction adenoviral array for the functional analysis of shRNA libraries

“…A panel of seven adenoviral vectors expressing shRNAs directed against human protein kinases was applied to analyze the phenotypic response of tumor cells after gene silencing. The protein kinases selected are known to be involved in the regulation of apoptosis (BTK (Bruton's tyrosine kinase) [ 6 - 8 ], p38alpha (also known as MAPK14) [ 9 - 11 ]), in cell cycle progression (cyclin-dependent kinase 2, CDK2 [ 12 - 14 ], PKN1 [ 15 ]) or in mitotic regulation (NEK2 (also known as NIMA) [ 16 - 18 ] and PBK (PDZ binding kinase) [ 19 , 20 ]). Furthermore, one protein kinase was included for which no effect on apoptosis had been described (TrkA [ 21 ]).…”

“…The kinases analyzed have been chosen from two categories: On one hand, we have selected rather general interplayers, such as PBK and NEK2, which have been shown to be important for cell cycle progression, DNA damage, and mitotic regulation [ 16 , 17 , 19 , 20 ]. On the other hand, we have selected more specific interplayers: p38alpha, BTK, PKN1 and CDK2 [ 6 , 8 , 9 , 12 , 13 , 15 , 27 - 29 ]. The knock-down constructs against PBK and NEK2 drove both HUVEC and U-2 OS cells into apoptosis, consistent with their overall importance for cell cycle progression.…”

Kinomics platform using GBM tissue identifies BTK as being associated with higher patient survival

“…We demonstrated that BTK protein expression is associated with longer survival times in GBM. As BTK has been shown to serve as tumour suppressor gene (72,73,74,75), this may in part explain these findings that BTK is linked to a more favourable prognosis. Recent studies have proposed a potential mechanism of the "protective" role of BTK as a tumour suppressor (76,77,78).…”

Expression profiling in transformed human B cells: influence of Btk mutations and comparison to B cell lymphomas using filter and oligonucleotide arrays

“…Prior knowledge of the role of Btk in apoptosis [17,18] and cell survival [19,20] prompted us to study the gene expression profile to gain further information regarding the cell signaling mechanisms of Btk. The 11 genes that were found to be induced in the XLA1 cell line included Btk itself, raising the possibility that the absence of functional Btk may be associated with up-regulation of certain genes and an autoregulatory effect on itself.…”

“…Phosphatidylinositol 3-kinase is required for the translocation of Btk to the membrane [15,16]. We and others have demonstrated the role of Btk in apoptosis [17,18]. Furthermore, the role of Btk as a dual regulator has been reviewed [19,20].…”