Btbd7 contributes to reduced E-cadherin expression and predicts poor prognosis in non-small cell lung cancer

Abstract

BackgroundDisorders of cell adhesion are critical steps in cancer progression in which varieties of markers including cadherins are involved in.Btbd7 was found to inhibit E-cadherin expression in MDCK cells and play important roles during branching morphogenesis of embryonic salivary glands and lungs. However its function in malignant tumors is largely unknown. The aim of this study is to investigate the clinicopathological significance and possible function of Btbd7 in non-small cell lung cancer.MethodsImmunohistochemistry and Western blotting were used to investigate Btbd7 expression in non-small cell lung cancer and lung tissues. The clinicopathological association and the overall survival was analyzed. In vitro experiments were performed using siRNA to investigate the function of Btbd7 in lung cancer cells.ResultsBtbd7 expression was elevated in non-small cell lung cancer tissues compared to normal lung tissues. Increased Btbd7 expression was significantly associated with lymph node metastasis, reduced E-cadherin expression and patients’ poor clinical outcome. Downregulation of Btbd7 expression in lung cancer cells by siRNA significantly inhibits cancer cell invasion and effectively restores E-cadherin expression in cancer cell membrane.ConclusionsBtbd7 contributes to reduced expression of E-cadherin and may be a promising cancer marker in non-small cell lung cancer.Electronic supplementary materialThe online version of this article (doi:10.1186/1471-2407-14-704) contains supplementary material, which is available to authorized users.

RETRACTED: Btbd7 is essential for region-specific epithelial cell dynamics and branching morphogenesis in vivo

“…Btbd7 also promotes an epithelial-to-mesenchymal transition in cultured tumor cells, with an increase in invasive capability (Fan et al, 2014;Luo et al, 2015;Wang et al, 2014;Yang et al, 2016). Nevertheless, whether Btbd7 is a master regulator of branching morphogenesis in vivo for multiple organs remains unknown.…”

Tuning Hydrogel Properties to Promote the Assembly of Salivary Gland Spheroids in 3D

“…34–37 Our initial experiments show that MDCK cells formed multicellular spherical aggregates rapidly in all four types of DRC gels. Further morphological analysis of MDCK spheroids in DRC2, one of the disulfide-lean gel formulations with a 2-h G’ value of approximately 800 Pa, confirmed the ability of individually dispersed single cells to form these organized structures with central lumen.…”

SNAI2 and TWIST1 in lymph node progression in early stages of NSCLC patients

“…Previous studies on E-Cadherin expression in NSCLC tested by immunohistochemistry report conserved protein expression ranging from 32% to 88% in tumors depending on the IHC cutoff value. [22][23][24][25][26][27][28][29] Moreover, an association of E-Cadherin loss of IHC expression and the N+ status of patients was reported in several stages I to IV NSCLC cohorts. 23,24,[28][29][30][31][32][33] Two other studies that analyzed CDH1 expression in stage I to III NSCLC tumors reported an opposite association between CDH1 expression and N status, with one study reporting preserved CDH1 expression and the other study reporting lost CDH1 expression.…”

“…Expression of N-Cadherin in NSCLC has been studied by IHC in several NSCLC cohorts, with 9% to 43% of tumors showing overexpression. 22,[36][37][38] IHC expression of VIMENTIN was also previously investigated in NSCLC, with 7%-66% of overexpression depending on the IHC cutoff value. 16,24,27,29,30,[38][39][40][41][42][43][44][45] One study quantified CDH2 expression by RT-qPCR in 30 NSCLC (stages I to IV) and found that 67% of tumors had N-Cadherin overexpression, with a bad prognosis value.…”