Bta-miR-493 Inhibits Bovine Preadipocytes Differentiation by Targeting BMPR1A via the TGFβ/BMP and p38MAPK Signaling Pathways

Abstract

Fat deposition significantly impacts the meat yield and the meat quality of beef cattle, which is closely associated with the preadipocyte proliferation and differentiation. Bta-miR-493 is expressed differentially in the backfat of Qinchuan cattle of various ages, and it may be involved in the fat accumulation of beef cattle. However, the role and functional mechanism of bta-miR-493 in fat deposition are still unclear. Tissue-specific expression analysis showed that the level of bta-miR-493 was moderately abundant in the adipose tissues of beef cattle. Moreover, the expression of bta-miR-493 in perirenal fat, subcutaneous fat, and longissimus dorsi muscle of Japanese black cattle was significantly higher than that in Qinchuan cattle. EdU staining, cell counting assay, and Oil Red O staining indicated that bta-miR-493 promoted the proliferation of bovine preadipocytes but inhibited their differentiation. The dual luciferase assay and transcriptomic analysis confirmed that bone morphogenetic protein receptor 1A (BMPR1A) is a target gene of bta-miR-493. Moreover, rescue experiments showed that bta-miR-493 could promote bovine preadipocyte proliferation but inhibit their differentiation by targeting BMPR1A via the TGFβ/BMP and p38MAPK signaling pathways. Overall, our findings revealed a bta-miR-493-BMPR1A-TGFβ/BMP/p38MAPK regulatory axis that will serve as a theoretical foundation for the molecular breeding of beef cattle with high intramuscular fat deposition.

Targeting Metabolic Syndrome Pathways: Carrot microRNAs As Potential Modulators

“…Similarly, Huang et al reported that the knockdown of SMAD4 disrupts the BMP2/4-induced adipogenic commitment process in the same cellular model . Long et al demonstrated that inhibition of upstream components, such as BMPR1 promotes preadipocyte proliferation but inhibits their differentiation in a culture of bovine preadipocytes …”

“… 41 Long et al demonstrated that inhibition of upstream components, such as BMPR1 promotes preadipocyte proliferation but inhibits their differentiation in a culture of bovine preadipocytes. 42 …”

“…41 Long et al demonstrated that inhibition of upstream components, such as BMPR1 promotes preadipocyte proliferation but inhibits their differentiation in a culture of bovine preadipocytes. 42 In addition, the ata-miR166b-5p potentially targets SMAD3, a multifaceted regulator in adipose physiology and the pathogenesis of obesity and type 2 diabetes mediated by the TGF-β1/SMAD3 signaling. 43 In vivo studies have suggested that blocking the TGF-β1/SMAD3 signaling represents a holistic approach to treating metabolic syndrome factors since it may protect from obesity, diabetes, and hepatic steatosis.…”

miR-129 Regulates Yak Intramuscular Preadipocyte Proliferation and Differentiation through the PI3K/AKT Pathway

“…However, the low IMF content of yak meat has become a major bottleneck, restricting high-quality meat development in the yak industry. Recent studies report that non-coding RNAs (ncRNAs) regulate key aspects of lipid metabolism [ 4 , 5 , 6 , 7 ].…”