Bt corn plants and their straw have no apparent impact on soil microbial communities

Tan, Fengxiao; Wang, Jianwu; Feng, Ying; Chi, Guoliang; Kong, Hailan; Qiu, Huifeng; Wei, Suling

doi:10.1007/s11104-009-0163-2

Cited by 43 publications

(24 citation statements)

References 55 publications

(64 reference statements)

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“…In general, our results are in line with several investigations proving that GMP do not have significant impact on rhizosphere or bulk soil microbial populations (Gschwendtner et al, 2010;Hannula et al, 2010;MiethlingGraff et al, 2010;Tan et al, 2010;Weinert et al, 2010), although in these investigations potatoes and maize have been used. The impact of genetically modified potatoes on soil and rhizosphere microorganisms has been studied in several investigations.…”

Section: Impact Of Barley (Hordeum Vulgare L) Transgenic Line H2282supporting

confidence: 92%

“…Amplification was done with primers REVB and FORB specific for prokaryotic 16S ribosomal ribonucleic acid serving as internal PCR amplification control (1500 bp), and with primers Rpg1 Ex3cw2 and Rpg1 Ex4ccw2 specific to third and fourth exon of Rpg1 gene (500 bp). structure of Bt-maize and conventional cultivars using cultivation independent profiling of 16S rRNA genes (Miethling-Graff et al, 2010) and 16S and 18S rRNA genes (Tan et al, 2010).…”

Section: Impact Of Barley (Hordeum Vulgare L) Transgenic Line H2282mentioning

confidence: 99%

“…and decreased numbers of fluorescent pseudomonads in comparison to nonresistant plants or to glyphosate-resistant plants not treated with glyphosate, and low ratio of Mn reducers to Mn oxidizers in the rhizosphere of glyphosate-resistant soybean (Kremer, Means, 2009). Several investigations have proved that GMP do not have significant impact on rhizosphere or bulk soil microbial populations (Gschwendtner et al, 2010;Hannula et al, 2010;Miethling-Graff et al, 2010;Tan et al, 2010;Weinert et al, 2010). Another concern regarding environmental biosafety of GMP is possibility of horizontal gene transfer (HGT) from transgenic plants to soil bacteria that could theoretically facilitate spread of antibiotic resistance genes in soil.…”

Section: Introductionmentioning

confidence: 99%

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Impact of barley (Hordeum vulgare L.) transgenic line H228.2A on substrate and rhizosphere microorganisms and the possibility of horizontal gene transfer

Grantina-Ievina

Minova

Rostoks

2013

Zemdirbyste-Agriculture

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Numerous investigations have been carried out to determine the impact of genetically modified plants, such as potato, maize, alfalfa and tobacco, on soil microorganisms and the results are contradictory. We applied classical microbiology methods to study quantitative changes of bacterial and fungal abundance in substrate and rhizosphere from barley (Hordeum vulgare L.) transgenic line H228.2A containing Rpg1 and bar genes, and its parent nontransgenic variety 'Golden Promise'. In addition, molecular biology methods were used to determine, if horizontal gene transfer from barley transgenic line to soil bacteria has occurred under experimental conditions by screening bacterial genomes for the presence of Rpg1 gene sequences using polymerase chain reactions. Results did not show significant impact on substrate and rhizosphere microorganisms. In the second pot experiment the average number of filamentous fungi in the rhizosphere of parent line 'Golden Promise' was higher than in the rhizosphere of transgenic line, but the variation among samples was very high probably caused by the high variation of substrate moisture content. None of bacterial deoxyribonucleic acid (DNA) samples isolated from substrate of rhizosphere of barley transgenic line resulted in positive amplification of Rpg1 gene-specific primer product.

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Section: Impact Of Barley (Hordeum Vulgare L) Transgenic Line H2282supporting

confidence: 92%

Section: Impact Of Barley (Hordeum Vulgare L) Transgenic Line H2282mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Impact of barley (Hordeum vulgare L.) transgenic line H228.2A on substrate and rhizosphere microorganisms and the possibility of horizontal gene transfer

Grantina-Ievina

Minova

Rostoks

2013

Zemdirbyste-Agriculture

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“…Furthermore other soil organisms interacting with earthworms might be affected. Modification, limitation or even loss of ecosystem services and functioning are potential consequences (Tan et al, 2010). Ecological risk assessments (ERA) of GE plants involves generating, collecting and assessing information on a GE plant in order to determine its potential adverse impact relative to its non-GE plant comparator, and thus assessing its comparative safety (EFSA, 2010).…”

Section: Introductionmentioning

confidence: 99%

Genetically Engineered Antifungal Wheat has no Detrimental Effects on the Key Soil Species Lumbricus terrestris

Lindfeld¹,

Nentwig

2012

TOECOLJ

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Abstract:Since the first introduction of genetically engineered (GE) plants, one of the major concerns has been their potential effects on non-target organisms and ecosystem services. We focused in this study on the earthworm species Lumbricus terrestris as important ecosystem engineer and studied its performance when feeding on GE antifungal wheat. We compared litter consumption, weight change and mortality of individuals feeding on GE wheat with either specific resistance against powdery mildew (Blumeria graminis) or unspecific resistance against fungi via chitinase and glucanase expression with individuals feeding on non-GE wheat or other conventional crops. We did not find detrimental direct or indirect effects of GE wheat on L. terrestris and overall L. terrrestris tended to cope even better with GE wheat varieties. Concluding from our experiment the transgene products do not harm the soil key species L. terrestris and ecosystem services like decomposition, organic matter turnover and nutrient cycling are unlikely to be affected detrimentally.

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“…Assim quaisquer alterações, ainda que pequenas, na comunidade microbiana do solo devem ser consideradas (TAN et al, 2010). …”

Section: Introductionunclassified

Ocorrência e persistência de fragmentos de transgenia (milho Bt evento MON810) em solos agrícolas brasileiros e avaliação de sua comunidade microbiana

Ferrari¹

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Aos meus amigos, Enéas, Gustavo, Letícia Mizuhira, Marília, Marina, Marcela, Naissa, Andressa, Aline, Caio, Felippe, Fernandinha e Rosineide pela amizade e por toda a ajuda e por estarem sempre presentes, na alegria e na tristeza.A todos que de alguma forma contribuíram com a execução deste trabalho. Obrigada!!! O uso de culturas GM (geneticamente modificadas) tem sido questionado quanto ao destino dos produtos derivados da transgenia no ambiente. Com a liberação de exsudatos das raízes das plantas e a decomposição dos resíduos culturais, aumenta-se a quantidade de DNA transgênico no ambiente, que pode ser adsorvido à superfície ativa das partículas do solo e/ou degradado pela ação de enzimas microbianas. A comunidade microbiana do solo pode entrar em contato direto com estes produtos, aumentando a probabilidade de transferência horizontal de fragmentos de DNA transgênico para os microrganismos. Também, alterações na composição dos exsudatos das plantas GM e mudanças em função das práticas de manejo, podem resultar em alterações na composição funcional e estrutural da comunidade microbiana. Assim, faz-se necessário avaliar a persistência dos produtos derivados da transgenia no solo e seus possíveis efeitos sobre a comunidade microbiana. Os objetivos deste estudo foram: avaliar a persistência dos fragmentos 35S-hsp70, hsp70-cry1Ab e cry1Ab-planta da construção gênica do milho Bt (evento MON810) em diferentes tipos de solo e temperaturas, em condições de microcosmo e de campo; e determinar a abundância do número de cópias dos gene 16S rRNA de Bacteria, Firmicutes, Verrucomicrobria e Archaea, e 18S rRNA de Fungo nas mesmas condições, e avaliar a estrutura da comunidade bacteriana em áreas agrícolas de cultivo de milho Bt. No primeiro estudo, o DNA do milho Bt MON810 foi adicionado em solos arenoso e argiloso. Como controle negativo, apenas água estéril foi misturada ao solo. Amostras de solo foram incubadas a 15 e 25ºC. Em campo, os solos foram amostrados em três áreas agrícolas em Fátima do Sul, MS, em dois anos consecutivos. Após extração de DNA, os fragmentos foram quantificados por qPCR. No segundo estudo, foram determinadas a abundância dos genes 16S rRNA de Bacteria, Firmicutes, Verrucomicrobria e Archaea e 18S rRNA de Fungo e avaliada a estrutura da comunidade bacteriana por T-RLFP. Os resultados mostraram que em condições de microcosmo, os fragmentos hsp70-cry1Ab e cry1Ab-planta persistiram até 291 dias, e o fragmento 35S-hsp70 até 180 dias. A temperatura e o tipo de solo não afetaram a persistência dos fragmentos. Em campo, o número de cópias desses fragmentos foi maior na segunda coleta. No segundo estudo, o número de cópias do gene 16S rRNA de Bacteria aumentou com adição de DNA do milho Bt nos microcosmos, e uma redução do número de cópias foi verificada para Archaea, Verrucomicrobia e Fungo. Para Firmicutes, os resultados não foram consistentes. As temperaturas não resultaram em efeito na abundância dos genes, enquanto o tipo de solo teve efeito apenas para Archaea e Verrucomicrobia. Áreas agrícolas co...

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Bt corn plants and their straw have no apparent impact on soil microbial communities

Cited by 43 publications

References 55 publications

Impact of barley (Hordeum vulgare L.) transgenic line H228.2A on substrate and rhizosphere microorganisms and the possibility of horizontal gene transfer

Impact of barley (Hordeum vulgare L.) transgenic line H228.2A on substrate and rhizosphere microorganisms and the possibility of horizontal gene transfer

Genetically Engineered Antifungal Wheat has no Detrimental Effects on the Key Soil Species Lumbricus terrestris

Ocorrência e persistência de fragmentos de transgenia (milho Bt evento MON810) em solos agrícolas brasileiros e avaliação de sua comunidade microbiana

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