BT cationic peptides: Small peptides that modulate innate immune responses of chicken heterophils and monocytes

Kogut, Michael H.; Genovese, Kenneth J.; He, Haiqi; Swaggerty, Christina L.; Jiang, Yi

doi:10.1016/j.vetimm.2011.10.023

Cited by 15 publications

(8 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Although it is not the same organic acid evaluated in our study, dietary supplementation with ascorbic acid has been shown to increase in vitro heterophil function, specifically the ability to kill bacteria ( Andreasen and Frank, 1999 ). Additional studies have also shown feeding prebiotics or antimicrobial peptides to young chicks can also enhance heterophil functional efficiency ( Lowry et al., 2005 , Kogut et al., 2012 ). Our data also indicate the supplement's affect went beyond the gut and had a direct impact on PBL function including heterophil-mediated degranulation and oxidative burst.…”

Section: Discussionmentioning

confidence: 99%

“…Other feed additive studies support the idea of a “primed” immune system. For example, supplementing the diet of young chicks with cationic peptides increases heterophil and monocyte mRNA expression of key proinflammatory cytokines and chemokines after stimulation ( Kogut et al., 2012 ), which translates to increased resistance against Salmonella colonization in a challenge model ( Kogut et al., 2013 ). Outside of the studies conducted by our laboratory, the authors are unaware of reports addressing PBL activity in broilers given a feed additive containing organic acids and botanicals, so most comparisons discussed herein are related to data generated in specific tissues and not PBL.…”

Section: Discussionmentioning

confidence: 99%

“…Over the years, our laboratories have used an array of diverse feed additives to help protect chickens of all ages against key foodborne pathogens including Salmonella ( Lowry et al., 2005 , Grilli et al., 2011 , Kogut et al., 2012 , Kogut et al., 2013 ) and Campylobacter ( Grilli et al., 2013 ). Administration of prebiotics or antimicrobial peptides to young chicks enhances heterophil functional efficiency that corresponds to increased resistance to colonization and organ invasion by Salmonella ( Lowry et al., 2005 , Kogut et al., 2012 , Kogut et al., 2013 ). The poultry industry has used organic acids in the diet and water supply to enhance growth and performance for many years ( Khan and Iqbal, 2016 ) which significantly lowered Salmonella ( Grilli et al., 2011 ) and Campylobacter colonization in market-age broilers ( Grilli et al., 2013 ).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A microencapsulated feed additive containing organic acids, thymol, and vanillin increases in vitro functional activity of peripheral blood leukocytes from broiler chicks

Swaggerty

Genovese

et al. 2020

Poultry Science

Self Cite

View full text Add to dashboard Cite

During the first week after hatch, young chicks are vulnerable to pathogens as the immune system is not fully developed. The objectives of this study were to determine if supplementing the starter diet with a microencapsulated feed additive containing citric and sorbic acids, thymol, and vanillin affects in vitro functional activity of peripheral blood leukocytes ( PBLs ). Day-old chicks (n = 800) were assigned to either a control diet (0 g/metric ton [ MT ]) or a diet supplemented with 500 g/MT of the microencapsulated additive. At 4 D of age, peripheral blood was collected (100 birds per treatment), and heterophils and monocytes isolated (n = 4). Heterophils were assayed for the ability to undergo degranulation and production of an oxidative burst response while nitric oxide production was measured in monocytes. Select cytokine and chemokine mRNA expression levels were also determined. Statistical analysis was performed using Student t test comparing the supplemented diet to the control ( P ≤ 0.05). Heterophils isolated from chicks fed the microencapsulated citric and sorbic acids, thymol, and vanillin had higher ( P ≤ 0.05) levels of degranulation and oxidative burst responses than those isolated from chicks on the control diet. Heterophils from the supplemented chicks also had greater ( P ≤ 0.05) expression of IL10, IL1β, and CXCL8 mRNA than those from control-fed chicks. Similarly, nitric oxide production was significantly ( P ≤ 0.05) higher in monocytes isolated from birds fed the supplement. The cytokine and chemokine profile in monocytes from the supplement-fed chicks showed a significant ( P ≤ 0.05) drop in IL10 mRNA expression while IL1β, IL4, and CXCL8 were unchanged. In conclusion, 4 D of supplementation with a microencapsulated blend made up of citric and sorbic acids, thymol, and vanillin enhanced the in vitro PBL functions of degranulation, oxidative burst, and nitric oxide production compared with the control diet. Collectively, the data suggest feeding broiler chicks a diet supplemented with a microencapsulated blend of citric and sorbic acids, thymol, and vanillin may prime key immune cells making them more functionally efficient and acts as an immune-modulator to boost the inefficient and undeveloped immune system of young chicks.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A microencapsulated feed additive containing organic acids, thymol, and vanillin increases in vitro functional activity of peripheral blood leukocytes from broiler chicks

Swaggerty

Genovese

et al. 2020

Poultry Science

Self Cite

View full text Add to dashboard Cite

show abstract

“…In a study by Nijnik et al, AMP IDR-1002 was observed to provide protection against E. coli infection and induce the secretion of the human peripheral blood monocytes chemokines CXCL1, CXCL2, CXCL7, and CXCL8 via a Gi-coupled receptor as well as the PI3K, NF-κB, and MAPK signaling pathways [21]. Kogut et al discovered that AMP BT downregulated the expression of proinflammatory cytokines (IL-1β and IL-6) and inflammatory chemokines (CXCLi1 and CXCLi2) by inhibiting the phosphorylation levels of proteins in the MAPK signaling pathway [22]. Moreover, LL-37 treatment enhanced the proliferation and migration of adipose-derived stromal/stem cells (ASCs) by activating MAPK, upregulating EGR1 expression, and promoting hair growth [23].…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effects of Antimicrobial Peptide JH-3 on Salmonella enterica Serovar Typhimurium Strain CVCC541 Infection-Induced Inflammatory Cytokine Release and Apoptosis in RAW264.7 Cells

et al. 2019

View full text Add to dashboard Cite

The antibiotic resistance of Salmonella has become increasingly serious due to the increased use of antibiotics, and antimicrobial peptides have been considered as an ideal antibiotic alternative. Salmonella can induce macrophage apoptosis and thus further damage the immune system. The antimicrobial peptide JH-3 has been shown to have a satisfactory anti-Salmonella effect in previous research, but its mechanism of action remains unknown. In this study, the effects of JH-3 on macrophages infected with Salmonella Typhimurium CVCC541 were evaluated at the cellular level. The results showed that JH-3 significantly alleviated the damage to macrophages caused by S. Typhi infection, reduced the release of lactic dehydrogenase (LDH), and killed the bacteria in macrophages. In addition, JH-3 decreased the phosphorylation level of p65 and the expression and secretion of interleukin 2 (IL-2), IL-6, and tumor necrosis factor-α (TNF-α) by inhibiting the activation of the mitogen-activated protein kinase (MAPK) (p38) signaling pathway and alleviating the cellular inflammatory response. From confocal laser scanning microscopy and flow cytometry assays, JH-3 was observed to inhibit the release of cytochrome c in the cytoplasm; the expression of TNF-αR2, caspase-9, and caspase-8; to further weaken caspase-3 activation; and to reduce the S.-Typhi-induced apoptosis of macrophages. In summary, the mechanism by which JH-3 inhibits Salmonella infection was systematically explored at the cellular level, laying the foundation for the development and utilization of JH-3 as a therapeutic alternative to antibiotics.

show abstract

“…Heterophils were isolated from the peripheral blood of 100 chickens per line 6 days post-hatch. Following blood collection, heterophils were isolated as previously described ( 14 ). Briefly, blood from chickens was collected in vacutainer tubes containing disodium ethylenediaminetetraacetic acid (EDTA) (BD vacutainer, Franklin Lakes, NJ, USA) and mixed thoroughly.…”

Section: Methodsmentioning

confidence: 99%

Critical Role of Glycogen Synthase Kinase-3Î² in Regulating the Avian Heterophil Response to Salmonella enterica Serovar Enteritidis

et al. 2014

Self Cite

View full text Add to dashboard Cite

A microarray-assisted gene expression screen of chicken heterophils revealed glycogen synthase kinase-3β (GSK-3β), a multifunctional Ser/Thr kinase, to be consistently upregulated 30–180 min following stimulation with Salmonella enterica serovar Enteritidis (S. Enteritidis). The present study was designed to delineate the role of GSK-3β in regulating the innate function of chicken heterophils in response to S. Enteritidis exposure. Using a specific GSK-3β ELISA assay, 30 min after infection with S. Enteritidis, heterophils had a significant decrease (p ≤ 0.05) in total GSK-3β, but a significant increase (p ≤ 0.05) in phosphorylated GSK-3β (Ser9). By 60 min post-infection, there was no difference in the amount of phosphorylated GSK-3β (Ser9) in either the uninfected and infected heterophils. S. Enteritidis interaction with heterophils alters GSK-3β activity by stimulating phosphorylation at Ser9 and that peaks by 30 min post-infection. Further, inhibition of GSK3β with lithium chloride resulted in a significant decrease (p ≤ 0.05) in NF-κB activation and expression of IL-6, but induces a significant increase (p ≤ 0.05) in the expression of the anti-inflammatory cytokine, IL-10. Using a phospho-specific antibody array confirmed the phosphorylation of GSK-3β (Ser9) as well as the phosphorylation of the downstream cytokine-activated intracellular signaling pathway involved in stimulating immune responses, IκB, the IκB subunit IKK-β, and the NF-κB subunits p105, p65, and c-Rel. Our data revealed that the phosphorylation of GSK-3β (Ser9) is responsible for inducing and controlling an innate response to the bacteria. Our findings suggest that the repression of GSK-3 activity is beneficial to the host cell and may act as a target for treatment in controlling intestinal colonization in chickens. Further experiments will define the in vivo modulation of GSK-3 as a potential alternative to antibiotics in salmonella and other intestinal bacterial infections.

show abstract

BT cationic peptides: Small peptides that modulate innate immune responses of chicken heterophils and monocytes

Cited by 15 publications

References 43 publications

A microencapsulated feed additive containing organic acids, thymol, and vanillin increases in vitro functional activity of peripheral blood leukocytes from broiler chicks

A microencapsulated feed additive containing organic acids, thymol, and vanillin increases in vitro functional activity of peripheral blood leukocytes from broiler chicks

Inhibitory Effects of Antimicrobial Peptide JH-3 on Salmonella enterica Serovar Typhimurium Strain CVCC541 Infection-Induced Inflammatory Cytokine Release and Apoptosis in RAW264.7 Cells

Critical Role of Glycogen Synthase Kinase-3Î² in Regulating the Avian Heterophil Response to Salmonella enterica Serovar Enteritidis

Contact Info

Product

Resources

About