BSL-3 Laboratory User Training Program at NUITM-KEMRI

Bundi, Martin; Miringu, Gabriel; Inoue, Satoshi; Muriithi, Betty; Ashur, Salame; Wandera, Ernest Apondi; Kathiiko, Cyrus; Odoyo, Erick; Narita, Chika; Kwalla, Allan; Galata, Amina; Makumi, Angela; Huka, Sora; Shah, Mohammed; Karama, Mohamed; Shimada, Muneaki; Bii, Cristine; Kariuki, Samuel; Horio, Masahiro; Ichinose, Yuki

doi:10.2149/tmh.2014-08

Cited by 7 publications

(9 citation statements)

References 3 publications

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“…The training program is delivered through lectures, demonstrations, and hands-on training [ 1 , 14 ]. Lectures are used to deliver theoretical content while demonstrations and hands-on training are used to administer the practical content.…”

Section: Training Proceduresmentioning

confidence: 99%

“…Only summary data was used in the manuscript. The training content can be viewed from [ 1 , 14 ]. There were no other materials applied or obtained towards development of the manuscript.…”

Section: Acknowledgementsmentioning

confidence: 99%

“…The NUITM-KEMRI biosafety training program was developed with a primary goal of training new biosafety level three (BSL-3) laboratory users before they begin working in the laboratory and to generally strengthen biosafety and biosecurity in research and diagnostic settings [ 1 ]. Containment laboratories are complex working environments, with a substantial potential of exposure of personnel and the public to infectious pathogens.…”

Section: Introductionmentioning

confidence: 99%

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Biosafety and biosecurity capacity building: insights from implementation of the NUITM-KEMRI biosafety training model

Muriithi

Bundi²,

Galata

et al. 2018

Trop Med Health

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The NUITM-KEMRI biosafety training program was developed for capacity building of new biosafety level three (BSL-3) laboratory users. The training program comprehensively covers biosafety and biosecurity theory and practice. Its training curriculum is based on the WHO biosafety guidelines, local biosafety standards, and ongoing biosafety level three research activities in the facility, also taking into consideration the emerging public health issues. The program’s training approach enhances the participant’s biosafety and biosecurity knowledge and builds their skills through the hands-on practice sessions and mentorship training. Subsequently, the trainees are able to integrate acquired knowledge and good practices into their routine laboratory procedures. This article describes implementation of the NUITM-KEMRI biosafety training program.

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Section: Training Proceduresmentioning

confidence: 99%

“…Only summary data was used in the manuscript. The training content can be viewed from [ 1 , 14 ]. There were no other materials applied or obtained towards development of the manuscript.…”

Section: Acknowledgementsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biosafety and biosecurity capacity building: insights from implementation of the NUITM-KEMRI biosafety training model

Muriithi

Bundi²,

Galata

et al. 2018

Trop Med Health

Self Cite

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“…The NUITM-KEMRI biosafety training program was used. 6 Usually, the training program is delivered through an annual workshop and divided into initial assessment, training phase, and final assessment sessions. In the initial assessment session, a written test is given to establish the participants' initial knowledge, which also helps to identify training needs.…”

Section: Methodsmentioning

confidence: 99%

Knowledge and Practices Regarding Usage of Biological Safety Cabinets

Miringu¹,

Bundi²,

Muriithi³

et al. 2017

Appl Biosaf.

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When properly used and adequately maintained, a biological safety cabinet (BSC) provides protection to the laboratory worker, the products, and the environment. Knowledge about proper use of the BSC is therefore important. This study evaluated BSC usage knowledge and practices and the effect of prior experience on training outcomes. Thirteen participants randomly selected from research centers were evaluated through a structured questionnaire covering biosafety considerations involved in using a BSC as per the World Health Organization's Laboratory Biosafety Manual, third edition. The questionnaire was administered before and after the training. Training was conducted using the NUITM-KEMRI biosafety training program, enhanced to include more modules on BSCs. Differences in the participants' knowledge and practices regarding BSC usage were observed. After training, the participants were able to give correct responses. Furthermore, participants who had fewer years of experience showed the greatest improvement. Results indicate the need for proper training of laboratory workers early in their careers, coupled with close supervision, mentorship, and regular refresher training.

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“…5 Briefly, these standards pertain to the following aspects: 1) formal training should consist of 3 elements, didactic or classroom style theoretical preparation, one-on-one practical training in the facility, and mentored on-the-job training; 2) certification of training; and 3) reevaluation of all persons working in the BSL-4 to ensure that their knowledge and skills remain current. This framework has served as a model for many BSL-3/4s globally 6 an introduction to the care and use of a protective suit or glove box; storage and record keeping of pathogens; clean-up and decontamination following procedures or spillage; solid and liquid waste management; use of autoclaves and other specialized equipment; communication with others inside and outside the facility; and other general procedures. Then the trainee is assigned a dedicated mentor and is introduced to working with live pathogens under his/her close supervision.…”

mentioning

confidence: 99%

Networking for training Level 3/4 biosafety laboratory staff

Huang

Han

et al. 2019

Journal of Biosafety and Biosecurity

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a b s t r a c tWorldwide, public health systems are continually challenged by emerging and re-emerging viruses. It is therefore important that high-containment labs coordinate and communicate globally to share their experiences and lessons to improve their capacity to respond to threats. The National Biosafety (Level 4) Laboratory in Wuhan, CAS, which is the first Level 4 biosafety laboratory (BSL-4) in China, has been certified recently and it is expected to play an important role in the prevention and control of highly infectious agents in future. Trained and experienced staff in such organizations is the most important factor contributing to safety and security. Therefore, it is imperative to develop a standard training program. Accordingly, in the present study, we developed an improved training program and assessment system based on policies and practices developed by BSL-3/4s in other countries. It included the following three components: (1) A flexible modularized theoretical training: This training comprised 14 modularized theoretical topics such that staffs with different backgrounds could take this theoretical training with different topic combinations according to their knowledge and skill levels; (2) A standardized practical training assessment: This comprehensive assessment, which could be used with biosafety laboratory staff before, during, and after training, included standard operation procedures to meet the special requirements of trainees with different scores; and (3) An applicable documentation system: A certification system was established to evaluate the ability of all staff working inside or outside the laboratory, implemented by a special committee. This certification was approved and authorized by the director of the laboratory and was classified into three grades with corresponding minimal requirements. Further, the present study examined the importance of and need for networking for training BSL-3/4 staff. The establishment of rigorous standards for training BSL-3/4 staff will instill confidence in the public, policy makers, and security officials. Additionally, the expanded international network of BSL-3/4s will continue to be operated safely and will pose no risk to scientific staff, local communities, surrounding environment, and host nations. The clarification and coordination of training standards will help develop a highly-qualified biocontainment workforce and will result in a series of programs that will enable scientists to develop measures to deal with existing threat agents and new diseases that emerge.

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BSL-3 Laboratory User Training Program at NUITM-KEMRI

Cited by 7 publications

References 3 publications

Biosafety and biosecurity capacity building: insights from implementation of the NUITM-KEMRI biosafety training model

Biosafety and biosecurity capacity building: insights from implementation of the NUITM-KEMRI biosafety training model

Knowledge and Practices Regarding Usage of Biological Safety Cabinets

Networking for training Level 3/4 biosafety laboratory staff

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