BseGI Restriction of the Polymerase Chain Reaction Amplicon Th444 Is Required to Distinguish Biotypes of Trichoderma aggressivum Causing Serious Losses in Mushroom (Agaricus bisporus) Production

Abstract

<jats:p>Green mold is a serious disease of the cultivated mushroom causing losses in production of economical importance. In the present study, digestion of a Th<jats:sub>444</jats:sub> amplicon with endonuclease <jats:italic>Bse</jats:italic>GI was useful to discriminate <jats:italic>Trichoderma aggressivum</jats:italic> f. <jats:italic>aggressivum</jats:italic> (<jats:italic>T.a.f.a</jats:italic>) from the <jats:italic>T. aggressivum</jats:italic> f. <jats:italic>europeanum</jats:italic> (<jats:italic>T.a.f.e</jats:italic>.). The informative restriction fragments of 260 and 300 bp were revealed in the corresponding reference strains <jats:italic>T.a.f.a</jats:italic>. and <jats:italic>T.a.f.e</jats:italic>. The 300-bp marker was found in all 28 Polish mushroom isolates tested.</jats:p>