BSA-lysozyme coated NaCa2HSi3O9 nanorods on titanium for cytocompatibility and antibacterial activity

Yang, Xue; Zhang, Lan; Han, Yuexin

doi:10.1016/j.jmst.2021.01.074

Cited by 10 publications

(14 citation statements)

References 38 publications

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“…Moreover, they can be synthesized via simple and fast methods, which makes them relatively cheap. It should also be noted that biopolymers can be introduced on the surface of metal oxides to increase their affinity to enzymes 7 , 24 . There is a great deal of information in the literature concerning materials based on chitosan/chitin/cellulose and inorganic oxides, and their application in enzyme immobilization 25 – 28 .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of MxOy/fucoidan hybrid system and their application in lipase immobilization process

Kołodziejczak-Radzimska

Bielejewski

Biadasz

et al. 2022

Sci Rep

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In this work, new MxOy/fucoidan hybrid systems were fabricated and applied in lipase immobilization. Magnesium (MgO) and zirconium (ZrO2) oxides were used as MxOy inorganic matrices. In the first step, the proposed oxides were functionalized with fucoidan from Fucus vesiculosus (Fuc). The obtained MgO/Fuc and ZrO2/Fuc hybrids were characterized by means of spectroscopic analyses, including Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy, and nuclear magnetic resonance. Additionally, thermogravimetric analysis was performed to determine the thermal stability of the hybrids. Based on the results, the mechanism of interaction between the oxide supports and fucoidan was also determined. Furthermore, the fabricated MxOy/fucoidan hybrid materials were used as supports for the immobilization of lipase from Aspergillus niger, and a model reaction (transformation of p-nitrophenyl palmitate to p-nitrophenol) was performed to determine the catalytic activity of the proposed biocatalytic system. In that reaction, the immobilized lipase exhibited high apparent and specific activity (145.5 U/gcatalyst and 1.58 U/mgenzyme for lipase immobilized on MgO/Fuc; 144.0 U/gcatalyst and 2.03 U/mgenzyme for lipase immobilized on ZrO2/Fuc). The immobilization efficiency was also confirmed using spectroscopic analyses (FTIR and XPS) and confocal microscopy.

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Section: Introductionmentioning

confidence: 99%

Evaluation of MxOy/fucoidan hybrid system and their application in lipase immobilization process

Kołodziejczak-Radzimska

Bielejewski

Biadasz

et al. 2022

Sci Rep

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“…The adhesion strengths of ZS and ZC were evaluated by scratch tests. The critical load of ZS was about 32 N (Figure S2(a)), and after Col-I covering treatment, it decreased to 23 N for ZC (Figure S2(b)) but was still suitable for implantation …”

Section: Resultsmentioning

confidence: 99%

“…The critical load of ZS was about 32 N (Figure S2(a)), and after Col-I covering treatment, it decreased to 23 N for ZC (Figure S2(b)) but was still suitable for implantation. 50 Biodegradation behaviors of Zn-based implants determine their performance after implantation, e.g., the cell response and resulting tissue integration. Corrosion behaviors of different surfaces were evaluated.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

An Extracellular Matrix-like Surface for Zn Alloy to Enhance Bone Regeneration

Mao

Zhu

Zhang

et al. 2022

ACS Appl. Mater. Interfaces

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Zn-based alloys are promising biodegradable implants for bone defect repair due to their good mechanical performance and degradability. However, local Zn2+ released from Zn-based implants can seriously affect adhering cell behaviors as well as new bone formation on implant surfaces. To address this issue, we have fabricated a bone-mimetic extracellular matrix (ECM)-like surface on Zn-1Ca implants using a hybrid process of anodization, hydrothermal treatment (HT), and fluorous-curing. The ECM-like surface consisted of Zn2SiO4 nanorods layered with collagen I (Col-I). The Zn2SiO4 nanorods were hemicrystallized and transformed by the reaction of Zn(OH)2 and SiO4 4– during the HT. The Zn2SiO4 nanorods effectively protected the substrate from corrosion; the Col-I layer decreased the degradation of Zn2SiO4 nanorods and further reduced Zn2+ release into the medium. This ECM-like surface generated a microenvironment with appropriate Zn2+ levels, nanorod-like topography, and Col-I. It significantly improved adhesion, proliferation, and differentiation of osteoblasts on implant surfaces and vascularization of endothelial cells in the extract medium. The in vivo results are in good agreement with in vitro tests, with the ECM-like surface significantly enhancing new bone formation and bone-implant contact compared to the bare implant surface. Overall, this bone-mimetic ECM-like material of Col-I layered Zn2SiO4 nanorods is a promising scaffold that promotes the bone regeneration of Zn-based implants.

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“…They are 284.1, 284.6, 286.1, 286.5, and 288.4 eV, which are attributed to CC, C–C, C–N, C–O, and CO, respectively. Thermal treatments (not higher than 180 °C) of polypeptides and proteins, for example, collagen, lysozyme, and so forth are known to induce intermolecular cross-linking between two or more molecules via carboxylic acids and amines instead of carbon–carbon double bonds. ,, The increased amount of CC on the HCA x surface is contributed by tryptophan in HHC-36. Furthermore, high-resolution spectra of N 1s spectra were resolved into two peaks at 399.8 and 401.9 eV (Figure c4), which are attributed to N–C–O and N–C in proteins, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…The bonding strength of the composite coating can meet the requirement well when the implants service in vivo. 39 Moreover, the mechanical properties of Ti substrates was not affected obviously after thermal treatment in PFHP, 29,40 indicating that ECM coating will be a potential coating applied on Ti implants.…”

Section: Preliminary Antibacterial Evaluation Of Ca Nanonets In Vitromentioning

confidence: 99%

pH-Responsive ECM Coating on Ti Implants for Antibiosis in Reinfected Models

Zhang

et al. 2021

ACS Appl. Bio Mater.

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Reinfection of implants during their service life causes troubles to patients. Traditionally, physical loading or chemical bonding of antibacterial agents on implant surfaces cannot settle the repeated bacterial invasion after a period of implantation. In this work, a pH-responsive extracellular matrix (ECM) coating was fabricated on Ti. It consisted of hydroxyapatite (HA) nanorods, antimicrobial peptide (AMP) cross-linked collagen I nanonets (CA nanonets), and physically loaded AMPs. CA nanonets formed in the interspaces of HA nanorods and had an average pore size of 46.5 nm. With the increase in the weight ratio of AMP cross-linkers in collagen I (from 0 to 1:3), the isoelectric points of CA nanonets increased. CA nanonets linked with 50 wt % of AMPs (HCA1) had an isoelectric point of about 7, and their zeta potential shifted from electronegativity to electropositivity when the pH value changed from 7.4 to 6.0. Compared with other nanonets, HCA1 showed a pH-responsive blast release of physically loaded AMPs. It was due to the electrostatic repulsion between the physically adsorbed AMPs and HCA1 after a shift in the potential. In vitro, all the CA nanonets were cytocompatible and exhibited significant short-term antibacterial performance; however, just HCA1 showed outstanding long-time responsive antibacterial activity; in vivo, HCA1 inhibited bacterial infection and suppressed the inflammatory response, especially in a reinfected model, indicating its potential application in Ti implants to mitigate the risk of reinfection.

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BSA-lysozyme coated NaCa2HSi3O9 nanorods on titanium for cytocompatibility and antibacterial activity

Cited by 10 publications

References 38 publications

Evaluation of MxOy/fucoidan hybrid system and their application in lipase immobilization process

Evaluation of MxOy/fucoidan hybrid system and their application in lipase immobilization process

An Extracellular Matrix-like Surface for Zn Alloy to Enhance Bone Regeneration

pH-Responsive ECM Coating on Ti Implants for Antibiosis in Reinfected Models

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