BSA interaction and DNA cleavage studies of anti-bacterial benzothiazol-2-yl-malonaldehyde

Katrahalli, Umesha; Yallur, Basappa C.; Hadagali, Manjunatha D.; Krishna, P. Murali

doi:10.1016/j.molstruc.2019.06.062

Cited by 13 publications

(8 citation statements)

References 41 publications

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“…Next, 5 µL of each concentration were mixed in equal volume of M. phaseolina DNA in separate vials and incubated at 37°C for 24 and 48 h. A control was also set for comparison. Thereafter, all the treatments were loaded on 1% agarose gel and run for 45 min at 100 volts to examine the extent of DNA degradation (Katrahalli et al 2019).…”

Section: Dna Degradation Studymentioning

confidence: 99%

Trichoderma viride Controls Macrophomina phaseolina through its DNA disintegration and Production of Antifungal Compounds

Khan¹

2021

IJAB

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Macrophomina phaseolina is a highly destructive pathogen of more than 500 plant species. It is difficult to eradicate it through chemical means as no patented fungicide is available against this pathogen. Biological control is the possible alternative method for its suitable management. The present study was carried out to evaluate the biocontrol potential of five Trichoderma spp. against M. phaseolina and the possible mechanisms of action. Identifications of all the Trichoderma spp. viz. T. hamatum, T. harzianum, T. koningii, T. longipile and T. viride were confirmed on molecular basis by using two universal primer pairs namely ITS and EF1. Their biocontrol potential was evaluated in dual culture plate method where T. viride showed the highest inhibitory efficacy (63%) against M. phaseolina. T. koningii, T. hamatum and T. longipile showed akin effects by arresting growth of the pathogen by 46–47% followed by T. harzianum (28%). To find out the mechanisms of action, secondary extrolites of the best biocontrol fungus T. viride were tested against the pathogenic genomic DNA where all the concentrations partially degraded DNA bands after 24 h of incubation and a complete DNA band disappearance was noted after 48 h incubation. In addition, T. viride culture filtrates were partitioned with chloroform and ethyl acetate and subjected to GC-MS analysis for identification of potential antifungal constituents. The most abundant identified volatile compounds in the two organic solvent fractions were 9,12-octadecadienoic acid (Z,Z)- (44.54%), n-hexadecanoic acid (24.02%), hexadecanoic acid, 2-hydroxy-1-(hydroxymethyl) ethyl ester (14.25%), 9-tricosene, (Z)- (10.43%) and [1,1'-bicyclopropyl]-2-octanoic acid, 2'-hexyl-, methyl ester (10.43%). To conclude, T. viride was the best biocontrol agent against M. phaseolina and acts against the pathogen by DNA disintegration and production of antifungal secondary metabolites. © 2021 Friends Science Publishers

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Section: Dna Degradation Studymentioning

confidence: 99%

Trichoderma viride Controls Macrophomina phaseolina through its DNA disintegration and Production of Antifungal Compounds

Khan¹

2021

IJAB

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“…The overlap of the BSA fluorescence emission and cloxyquin absorption spectra is represented in Figure 7. The distance (r) between cloxyquin and Trp was calculated based on Equations (6)- (8) with the assumption parameters of BSA-ligand interaction that n and φ D are equal to 1.336 and 0.15, respectively [22,36]. The k 2 factor can range from 0 to 4 depending on the relative orientation of donor and acceptor.…”

Section: Fluorescence Resonance Energy Transfer (Fret) Investigationmentioning

confidence: 99%

Insight into the Molecular Interaction of Cloxyquin (5-chloro-8-hydroxyquinoline) with Bovine Serum Albumin: Biophysical Analysis and Computational Simulation

Phopin

Ruankham

Prachayasittikul

et al. 2019

IJMS

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Cloxyquin is a potential therapeutic compound possessing various bioactivities, especially antibacterial, antifungal, cardioprotective, and pain relief activities. Herein, the interaction mechanism between cloxyquin and bovine serum albumin (BSA) has been elucidated in order to fulfill its pharmacokinetic and pharmacodynamic gaps essential for further development as a therapeutic drug. Multi-spectroscopic and biophysical model analysis suggested that cloxyquin interacts with BSA via a static process by ground-state complex formation. Its binding behavior emerged as a biphasic fashion with a moderate binding constant at the level of 104 M−1. Thermodynamic analysis and molecular docking simulation concurrently revealed that hydrophobic interaction is a major driving force for BSA–cloxyquin complexation. Binding of cloxyquin tends to slightly enlarge the monomeric size of BSA without a significant increase of aggregate fraction. Cloxyquin preferentially binds into the fatty acid binding site 5 (FA5) of the BSA via hydrophobic interaction amongst its quinoline scaffold and Phe550, Leu531, and Leu574 residues of BSA. The quinoline ring and hydroxyl moiety of cloxyquin also form the π–π interaction and the hydrogen bond with Phe506. Our data indicate a potential function of serum albumin as a carrier of cloxyquin in blood circulation.

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“…Structurally, the human serum albumin protein has several domains, with secondary binding sites capable of interacting and transporting metallodrugs for their biodistribution. In order to study these bindings, HSA is normally replaced by bovine analogous (BSA) as a model protein because of its similarity, commercial availability, and better fluorescence properties [ 62 ]. Although other fluorophores are present, BSA fluorescence is mainly due to two tryptophan residues [ 63 , 64 ].…”

Section: Resultsmentioning

confidence: 99%

Selective Anticancer and Antimicrobial Metallodrugs Based on Gold(III) Dithiocarbamate Complexes

et al. 2021

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New dithiocarbamate cycloaurated complexes have been synthesized and their physicochemical and in vitro antitumor properties have been evaluated. All the performed studies highlighted good transport through the blood and biodistribution, according to the balance between the properties of hydrophilicity/lipophilicity and the binding of moderate strength to the BSA protein. Furthermore, none of the complexes exhibited reduction or decomposition reactions, presenting excellent physiological stability. The in vitro cytotoxic effect was evaluated on human colon cancer cell line Caco-2/TC7, and the complexes showed great antiproliferative activity and excellent selectivity, as much less effect was detected on normal Caco-2/TC7 cells. Most of the complexes exhibit antiproliferative activity that was better than or similar to auranofin, and at least nine times better than that of cisplatin. Its action mechanism is still under discussion since no evidence of cell cycle arrest was found, but an antioxidant role was shown for some of the selective complexes. All complexes were also tested as antimicrobial drugs, exhibiting good activity towards S. aureus and E. coli. bacteria and C. albicans and C. neoformans fungi.

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BSA interaction and DNA cleavage studies of anti-bacterial benzothiazol-2-yl-malonaldehyde

Cited by 13 publications

References 41 publications

Trichoderma viride Controls Macrophomina phaseolina through its DNA disintegration and Production of Antifungal Compounds

Trichoderma viride Controls Macrophomina phaseolina through its DNA disintegration and Production of Antifungal Compounds

Insight into the Molecular Interaction of Cloxyquin (5-chloro-8-hydroxyquinoline) with Bovine Serum Albumin: Biophysical Analysis and Computational Simulation

Selective Anticancer and Antimicrobial Metallodrugs Based on Gold(III) Dithiocarbamate Complexes

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