Bryonia dioica aqueous extract induces apoptosis through mitochondrial intrinsic pathway in BL41 Burkitt's lymphoma cells

Benarba, Bachir; Meddah, B.; Aoues, Abdelkader

doi:10.1016/j.jep.2012.02.052

Cited by 39 publications

(20 citation statements)

References 39 publications

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“…Inducing cancer cell apoptosis is the key to cancer treatment, and recent studies have shown that many natural substances induce apoptosis as their primary anticancer mechanism (29–31). Therefore, apoptosis rate is considered an indicator of anticancer activity.…”

Section: Discussionmentioning

confidence: 99%

(−)-Epigallocatechingallate induces apoptosis in B lymphoma cells via caspase-dependent pathway and Bcl-2 family protein modulation

Wang

Xie

Yan

et al. 2015

International Journal of Oncology

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(−)-Epigallocatechingallate (EGCG) as a representative polyphenol has attracted increasing attention due to its diversified effects, especially its potential as an agent for the prevention or treatment of certain cancers. However, the molecular mechanisms of EGCG-induced apoptosis in B lymphoma cells are unclear. The aim of this study was to investigate the effect of EGCG on proliferation and apoptosis in the B lymphoma cell lines Jeko-1 and Raji, and determine the underlying mechanisms. Cell proliferation and cytotoxicity were determined by the cell counting kit (CCK-8) assay; apoptosis was assessed by flow cytometry using the Annexin V-PE/7AAD double staining; Fas, Bcl-2 and Bax mRNA expression levels were determined by real-time PCR; caspase activity was measured by the caspase activity assay kit; the expression levels of apoptosis-associated proteins were determined by western blot analysis. We demonstrated that EGCG induced growth inhibition and apoptosis in a dose- and time-dependent manner. In agreement, EGCG upregulated the mRNA expression of Fas and Bax while downregulating Bcl-2. Protein expression levels of Bax, activated caspase-3, -7, -8, and -9, and PARP were increased, while Bcl-2 protein levels were reduced by EGCG treatment. Taken together, EGCG induces B lymphoma cell apoptosis by triggering caspase-dependent intrinsic (mitochondrial) and extrinsic (death receptor) pathways. These findings suggest that EGCG may be a potential agent for the treatment of B lymphoma.

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Section: Discussionmentioning

confidence: 99%

(−)-Epigallocatechingallate induces apoptosis in B lymphoma cells via caspase-dependent pathway and Bcl-2 family protein modulation

Wang

Xie

Yan

et al. 2015

International Journal of Oncology

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“…Flow cytometric analysis demonstrated that A549 cells were arrested at the G2/M phase and apoptosis was induced by the mitochondrial pathway reducing the expression of Bcl-2 and Bcl-xL and enhancing the expression of Bax, Bad and Bak resulting in activation of caspase-9 and caspase-3. Aqueous extract of Bryonia dioica induced apoptosis through mitochondrial intrinsic pathway in BL41 Burkitt's lymphoma cells (Benarba et al 2012). Extract caused upregulation of caspase-3 and -9, cleavage of PARP and decreased mitochondria membrane potential leading to apoptosis.…”

Section: Mechanisms Of Apoptosismentioning

confidence: 94%

Phytoconstituents as apoptosis inducing agents: strategy to combat cancer

et al. 2015

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Advancement in the field of cancer molecular biology has aided researchers to develop various new chemopreventive agents which can target cancer cells exclusively. Cancer chemopreventive agents have proficiency to inhibit, reverse and delay process of carcinogenesis during its early and later course. Chemopreventive agents can act as antioxidative, antimutagenic/antigenotoxic, anti-inflammatory agents or via aiming various molecular targets in a cell to induce cell death. Apoptosis is a kind of cell death which shows various cellular morphological alterations such as cell shrinkage, blebbing of membrane, chromatin condensation, DNA fragmentation, formation of apoptotic bodies etc. Nowadays, apoptosis is being one of the new approaches for the identification and development of novel anticancer therapies. For centuries, plants are known to play part in daily routine from providing food to management of human health. In the last two decades, diverse phytochemicals and various botanical formulations have been characterized as agents that possess potential to execute cancer cells via inducing apoptosis. Data obtained from the research carried out globally pointed out that natural products are the potential candidates which have capability to combat cancer. In the present review, we surveyed literature on natural products which throws light on the mechanism through which these phytochemicals induce apoptosis in cancer cells.

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“…The roots of the former species have been used as an antiphlogistic, detoxicant and anodyne, while the whole plant was used for the treatment of sclerosis, nose cancer and uterus tumors 6 . The latter species B. dioïca was recommended for the treatment of various inflammatory conditions, bronchial complaints, asthma, intestinal ulcer, hypertension and arthritis 7 .…”

Section: Introductionmentioning

confidence: 99%

Fatty Acids Composition and Antibacterial Activity of <i>Aristolochia longa</i> L. and <i>Bryonia dioïca</i> Jacq. Growing Wild in Tunisia

Dhouioui

Boulila²,

Jemli³

et al. 2016

J. Oleo Sci.

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International audienceThe composition of the fatty acids of the roots and aerial parts of Aritolochia longa (Aristolacheae) and Bryonia dioica (Cucurbutaceae) was analyzed by gas chromatography (GC-FID) and gas chromatography-mass spectrometry (GC-MS). The oils extracted from the aerial parts of both species were rich in polyunsaturated fatty acids with the essential linolenic and linoleic acids being the most prominent compounds. Oleic and linoleic acids were the majors fatty acids in the roots of both species. Whatever the plant part analyzed and the species, the saturated fatty acids were predominantly composed of palmitic and stearic acids. The antibacterial activity, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the lipid extracts were determined against a panel of five bacterial strains. The results showed that the sensitivity to the lipid extracts was different for the test bacterial strains, and the susceptibility of gram positive bacteria was found to be greater than gram negative bacteria. The antibacterial activity of the root lipid extracts was particularly important against Enterococcus feacium (CMI value of 125 mu g/mL; CMB values > 250 mu g/mL) and Streptococcus agalactiae (CMI value of 125 mu g/mL; CMB values 250 mu g/mL for A. longa roots). These results indicate that A. longa and B. dioica could be considered as good sources of essential fatty acids which can act as natural antibacterial agents

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Bryonia dioica aqueous extract induces apoptosis through mitochondrial intrinsic pathway in BL41 Burkitt's lymphoma cells

Cited by 39 publications

References 39 publications

(−)-Epigallocatechingallate induces apoptosis in B lymphoma cells via caspase-dependent pathway and Bcl-2 family protein modulation

(−)-Epigallocatechingallate induces apoptosis in B lymphoma cells via caspase-dependent pathway and Bcl-2 family protein modulation

Phytoconstituents as apoptosis inducing agents: strategy to combat cancer

Fatty Acids Composition and Antibacterial Activity of <i>Aristolochia longa</i> L. and <i>Bryonia dioïca</i> Jacq. Growing Wild in Tunisia

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