Brush cytology of Herpes Simplex virus infection in oral mucosa: Use of the ThinPrep® processor

Kobayashi, Tadao K.; Ueda, Masami; Nishino, Toshihiro; Terasaki, Shinichiro; Kameyama, Tadamitsu

doi:10.1002/(sici)1097-0339(199801)18:1<71::aid-dc12>3.3.co;2-9

Cited by 4 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A large body of literature has evolved, documenting its use in fluid-based samples (body cavity effusions, urine, and cerebral spinal fluid), as well as lavage samples and fine-needle aspiration (FNA) from various sites. 5,[7][8][9] However, the utility of Thin-Prep for the evaluation of ICC has not been thoroughly Additionally, there is also minimal experience with the immunodetection of nuclear markers with ThinPrep. Hence we set out to compare the performance of ThinPrep with that of formalin-fixed cell blocks in ICC analysis.…”

Section: Discussionmentioning

confidence: 99%

Immunocytochemistry of serous effusion specimens: A comparison of ThinPrep® vs. cell block

Gong

Sun

Michael

et al. 2002

Diagnostic Cytopathology

View full text Add to dashboard Cite

The ThinPrep Processor is gaining popularity in both gynecologic and nongynecologic cytologic samples, including effusion specimens. We compared immunocytochemical results on ThinPrep and cell-block preparations from the same effusion specimen with antibodies commonly used in effusion cytopathology. Samples from 17 reactive effusions and 79 effusions with metastatic adenocarcinomas were each prepared as monolayer ThinPreps and formalin-fixed, paraffin-embedded cell blocks. All slides were immunostained with antibodies against intermediate filaments (cytokeratins 8 and 18, and vimentin), cytoplasmic or membrane proteins (EMA, polyclonal CEA, B72.3, and BerEP4), and nuclear antigens (Ki67, PCNA, and p53), using the avidin-biotin procedure. ThinPrep and cell-block slides were stained simultaneously, using identical protocols. Both preparations showed similar results with respect to frequency of positivity, intensity, and distribution of stain for all nonnuclear markers tested. However, for the three nuclear markers Ki67, PCNA, and p53, the frequency and intensity of reaction with ThinPrep were significantly lower than with the cell-block preparation, particularly in malignant effusions. These findings suggest that immunocytochemical results obtained with ThinPrep match those of cell block for most markers tested. However, cell-block preparation are superior to ThinPrep for nuclear markers (Ki67, PCNA, and p53).

show abstract

Section: Discussionmentioning

confidence: 99%

Immunocytochemistry of serous effusion specimens: A comparison of ThinPrep® vs. cell block

Gong

Sun

Michael

et al. 2002

Diagnostic Cytopathology

View full text Add to dashboard Cite

show abstract

“…6 However, a primary herpetic infection of the mouth may be confused with other oral ulcerative diseases. 6,8 A laboratory diagnosis is then required, especially in immunocompromized patients, who can show lesions occurring at any site intraorally, forming ulcers with a very atypical appearance. 9 The cytological presentation of HSV is characteristic.…”

Section: Herpes Virusesmentioning

confidence: 99%

“…9 The cytological presentation of HSV is characteristic. 8 The cytopathic effects of HSV-1 can be visualized by the Pap stain. Syncytial multinucleated giant cells are seen, with ÔballooningÕ of cytoplasm.…”

Section: Herpes Virusesmentioning

confidence: 99%

Usefulness of oral cytopathology in the diagnosis of infectious diseases

et al. 2010

View full text Add to dashboard Cite

In recent years, the incidence of oral opportunistic infections has increased, partly due to the widespread implementation of organ and bone marrow transplantation and the increase in the prevalence of human immunodeficiency virus (HIV) infection. Cytology can be used as a rapid, inexpensive and simple routine procedure in diagnosing infectious diseases of the mouth. Moreover, ancillary methods can be applied to cytological samples, increasing the specificity and sensitivity for the diagnosis of infectious diseases. This review describes the cytopathological features of the main viral, fungal, bacterial and parasitic infections of the mouth. Cytological techniques of specimen collection, identification of infectious agents by cytomorphological approaches and ancillary methods, and diagnostic pitfalls will be discussed.

show abstract

“…On the other hand, cellular changes suggestive of herpes simplex virus (HSV) infection have been noted in different body sites, [3][4][5] including oral lesions. 6 Rapid methods of viral diagnosis in clinical samples include routine cytopathological examination for diagnostic viral inclusions and groundglass nuclei and immunocytochemical detection of specific viral antigens, 6 nucleic acid hybridization, 3,5 and most recently, a DNA diagnostic method using the polymerase chain reaction (PCR). 7,8 This newly described molecular biology technique is well-suited for the diagnosis of infection processes.…”

Section: Abstract: Pemphigus; Hsv Infection; Oral Cytology; Polymeramentioning

confidence: 99%

Coexistence of Pemphigus vulgaris and herpes simplex virus infection in oral mucosa diagnosed by cytology, immunohistochemistry, and polymerase chain reaction

Takahashi¹,

Kobayashi²,

Suzuki³

et al. 1998

Diagn. Cytopathol.

Self Cite

View full text Add to dashboard Cite

The patient was a 53-yr-old woman, who presented in September 1994 with blisters on her oral mucosa, and later, numerous bullous lesions appeared on her lips, face, and head. The patient was admitted to the dermatological ward for further investigations. Laboratory examination was not remarkable except for immunoglobulin, i.e., IgA 581 mg/dl and IgG 2,504 mg/dl, while IgM levels were unremarkable. At about the same time, a dermatologist established the

show abstract

Brush cytology of Herpes Simplex virus infection in oral mucosa: Use of the ThinPrep® processor

Cited by 4 publications

References 0 publications

Immunocytochemistry of serous effusion specimens: A comparison of ThinPrep® vs. cell block

Immunocytochemistry of serous effusion specimens: A comparison of ThinPrep® vs. cell block

Usefulness of oral cytopathology in the diagnosis of infectious diseases

Coexistence of Pemphigus vulgaris and herpes simplex virus infection in oral mucosa diagnosed by cytology, immunohistochemistry, and polymerase chain reaction

Contact Info

Product

Resources

About