Brugia Malayi Microfilariae from the Peritoneal Cavity of Jirds Vary in Their Ability to Penetrate the Mosquito Midgut *

Af, Schrater; Pa, Rossignol; Hamill, Bruce; Wf, Piessens; Spielman, Andrew

doi:10.4269/ajtmh.1982.31.292

Cited by 11 publications

(3 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It is important to consider that both populations of MF used in this experiment were derived from the peritoneal cavities of infected gerbils. Although we have previously shown a dramatic difference in mosquito infectivity between peritoneally-derived immature and mature MF [2], [34], [35], it is clear that intraperitoneally-derived MF, regardless of age, are considerably less infective than those found in circulating blood [43]. It is possible that the transcriptional profile of mature circulating MF differs from those that are derived from the peritoneal cavity.…”

Section: Discussionmentioning

confidence: 79%

A Deep Sequencing Approach to Comparatively Analyze the Transcriptome of Lifecycle Stages of the Filarial Worm, Brugia malayi

et al. 2011

View full text Add to dashboard Cite

BackgroundDeveloping intervention strategies for the control of parasitic nematodes continues to be a significant challenge. Genomic and post-genomic approaches play an increasingly important role for providing fundamental molecular information about these parasites, thus enhancing basic as well as translational research. Here we report a comprehensive genome-wide survey of the developmental transcriptome of the human filarial parasite Brugia malayi.Methodology/Principal FindingsUsing deep sequencing, we profiled the transcriptome of eggs and embryos, immature (≤3 days of age) and mature microfilariae (MF), third- and fourth-stage larvae (L3 and L4), and adult male and female worms. Comparative analysis across these stages provided a detailed overview of the molecular repertoires that define and differentiate distinct lifecycle stages of the parasite. Genome-wide assessment of the overall transcriptional variability indicated that the cuticle collagen family and those implicated in molting exhibit noticeably dynamic stage-dependent patterns. Of particular interest was the identification of genes displaying sex-biased or germline-enriched profiles due to their potential involvement in reproductive processes. The study also revealed discrete transcriptional changes during larval development, namely those accompanying the maturation of MF and the L3 to L4 transition that are vital in establishing successful infection in mosquito vectors and vertebrate hosts, respectively.Conclusions/SignificanceCharacterization of the transcriptional program of the parasite's lifecycle is an important step toward understanding the developmental processes required for the infectious cycle. We find that the transcriptional program has a number of stage-specific pathways activated during worm development. In addition to advancing our understanding of transcriptome dynamics, these data will aid in the study of genome structure and organization by facilitating the identification of novel transcribed elements and splice variants.

show abstract

Section: Discussionmentioning

confidence: 79%

A Deep Sequencing Approach to Comparatively Analyze the Transcriptome of Lifecycle Stages of the Filarial Worm, Brugia malayi

et al. 2011

View full text Add to dashboard Cite

show abstract

“…The neighboring cells around the invaded site are dislocated from the basal lamina. This phenomenon has been observed before in several studies on mosquito-microWlaria interactions Philippon, 1969, 1970;Bain et al, 1976;Laurence, 1966;Bain and Brengues, 1972;Bain and Chabaud, 1975;Petit, 1978 andPetit andSpitalierKaveh, 1979;Schrater et al, 1982;Perrone and Spielman, 1986). Additionally, it has recently been demonstrated that midgut epithelial cells of mosquito vectors are damaged by invading ookinetes of Plasmodium sp.…”

Section: Discussionmentioning

confidence: 71%

“…Previous studies (Iyengar, 1936;Beatty, 1936, 1938;Schrater et al, 1982;Oda and Wada, 1980;Fuhrman and Piessens, 1985;Fuhrman et al, 1987;Bryan and Southgate, 1988a,b;Bryan et al, 1990 andNayar andKnight, 1995) showing microWlariae exsheathment before Fig. 5.…”

Section: Discussionmentioning

confidence: 95%