Brucellosis and the British Public

Williams, Eirian

doi:10.1016/s0140-6736(70)91801-5

Cited by 11 publications

(3 citation statements)

References 6 publications

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“…However, later, it was realized that non-pasteurized or uncooked animal products (milk, cheese, ice cream and liver) were responsible [4]. In addition, contact with the infected animal excretions through the respiratory tract or the skin in their natural environment and contamination in the laboratory has been shown to cause the transmission of the disease [5][6][7]. Brucella melitensis is the most common agent causing infection throughout the world.…”

Section: Discussionmentioning

confidence: 99%

Brucellosis: a rare cause of febrile neutropenia in acute myeloblastic leukemia

2010

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Brucellosis is a zoonotic disease and endemically seen in the Middle East, Eastern Europe and continental America. Febrile neutropenia related to Brucellosis has been reported only in a few cases. Brucella was cultured from the bone marrow of a 42-year-old woman who was admitted to hospital with symptoms of fever and fatigue and later diagnosed as acute myeloblastic leukemia (AML). The patient was treated for both AML and Brucellosis without any problems and discharged from the hospital after scheduling her follow-up visits. Brucellosis might be considered in the etiology of febrile neutropenia in endemic regions and must be treated effectively to prevent possible morbidity and mortality during or after chemotherapy.

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Section: Discussionmentioning

confidence: 99%

Brucellosis: a rare cause of febrile neutropenia in acute myeloblastic leukemia

2010

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“…In decreasing order of virulence, Brucella melitensis , Brucella suis , and Brucella abortus are the agents most commonly implicated in human brucellosis (Bossi et al, 2004; Franco et al, 2007; Lucero et al, 2008). Although horizontal human transmission is possible, the most important source of human brucellosis is respiratory or gastro-intestinal exposure to contaminated animal tissue or unpasteurized milk and dairy food products, respectively (Williams, 1970; Eckman, 1975; Taylor and Perdue, 1989; Chomel et al, 1994; Godfroid et al, 2005; Corbel et al, 2006; Mantur et al, 2007). After breaching mucosal barriers, Brucella spp.…”

Section: Introductionmentioning

confidence: 99%

Host-Brucella interactions and the Brucella genome as tools for subunit antigen discovery and immunization against brucellosis

Gomez¹,

Adams²,

Rice‐Ficht³

et al. 2013

Front. Cell. Infect. Microbiol.

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Vaccination is the most important approach to counteract infectious diseases. Thus, the development of new and improved vaccines for existing, emerging, and re-emerging diseases is an area of great interest to the scientific community and general public. Traditional approaches to subunit antigen discovery and vaccine development lack consideration for the critical aspects of public safety and activation of relevant protective host immunity. The availability of genomic sequences for pathogenic Brucella spp. and their hosts have led to development of systems-wide analytical tools that have provided a better understanding of host and pathogen physiology while also beginning to unravel the intricacies at the host-pathogen interface. Advances in pathogen biology, host immunology, and host-agent interactions have the potential to serve as a platform for the design and implementation of better-targeted antigen discovery approaches. With emphasis on Brucella spp., we probe the biological aspects of host and pathogen that merit consideration in the targeted design of subunit antigen discovery and vaccine development.

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“…Animals and their food products are the primary source of human infection where the mucosal routes are the most common modes of transmission through inhalation and ingestion of animal-derived contaminated materials, respectively [1], [2], [3], [4], [5], [6], [7]. Consistent with the food animal-human symbiotic relationship, brucellosis is one of the most common zoonotic diseases estimated at an annual incidence of 500,000 cases [8].…”

Section: Introductionmentioning

confidence: 99%

Immunogenic and Invasive Properties of Brucella melitensis 16M Outer Membrane Protein Vaccine Candidates Identified via a Reverse Vaccinology Approach

et al. 2013

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Brucella is the etiologic agent of brucellosis, one of the most common and widely distributed zoonotic diseases. Its highly infectious nature, the insidious, systemic, chronic, debilitating aspects of the disease and the lack of an approved vaccine for human use in the United States are features that make Brucella a viable threat to public health. One of the main impediments to vaccine development is identification of suitable antigens. In order to identify antigens that could potentially be used in a vaccine formulation, we describe a multi-step antigen selection approach. We initially used an algorithm (Vaxign) to predict ORF encoding outer membrane proteins with antigenic determinants. Differential gene expression during acute infection and published evidence for a role in virulence were used as criteria for down-selection of the candidate antigens that resulted from in silico prediction. This approach resulted in the identification of nine Brucella melitensis outer membrane proteins, 5 of which were recombinantly expressed and used for validation. Omp22 and Hia had the highest in silico scores for adhesin probability and also conferred invasive capacity to E. coli overexpressing recombinant proteins. With the exception of FlgK in the goat, all proteins reacted to pooled sera from exposed goats, mice, and humans. BtuB, Hia and FlgK stimulated a mixed Th1–Th2 response in splenocytes from immunized mice while BtuB and Hia elicited NO release from splenocytes of S19 immunized mice. The results support the applicability of the current approach to the identification of antigens with immunogenic and invasive properties. Studies to assess immunogenicity and protective efficacy of individual proteins in the mouse are currently underway.

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Brucellosis and the British Public

Cited by 11 publications

References 6 publications

Brucellosis: a rare cause of febrile neutropenia in acute myeloblastic leukemia

Brucellosis: a rare cause of febrile neutropenia in acute myeloblastic leukemia

Host-Brucella interactions and the Brucella genome as tools for subunit antigen discovery and immunization against brucellosis

Immunogenic and Invasive Properties of Brucella melitensis 16M Outer Membrane Protein Vaccine Candidates Identified via a Reverse Vaccinology Approach

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