Brucella Genomics: Macro and Micro Evolution

Suárez-Esquivel, Marcela; Chaves-Olarte, Esteban; Moreno, Edgardo; Guzmán-Verri, Caterina

doi:10.3390/ijms21207749

Cited by 44 publications

(36 citation statements)

References 176 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Phage proteins, insertional sequences predicted in accessory genome, and prophages predicted in the individual genome may have a role in the diversity of B. melitensis . Suárez-Esquivel et al (2020) reported that insertional sequences in Brucella spp. may have a role in diversity within the species.…”

Section: Core and Accessory Gene Compositionsmentioning

confidence: 99%

“…The B. melitensis genome is conserved since there is no other extrachromosomal material (plasmids), and there is limited report about the horizontal gene transfer, which may be due to its intracellular survival. So the other possible mechanism for the evolution of B. melitensis may be mutations so as to survive in a new geographical region and also to survive in a new host ( Tan et al, 2015 ; Suárez-Esquivel et al, 2020 ). Hence, identifying the SNPs can enable to know the evolution of B. melitensis strains.…”

Section: Core and Accessory Gene Compositionsmentioning

confidence: 99%

See 1 more Smart Citation

Genome Sequencing and Comparative Genomics of Indian Isolates of Brucella melitensis

et al. 2021

View full text Add to dashboard Cite

Brucella melitensis causes small ruminant brucellosis and a zoonotic pathogen prevalent worldwide. Whole genome phylogeny of all available B. melitensis genomes (n = 355) revealed that all Indian isolates (n = 16) clustered in the East Mediterranean lineage except the ADMAS-GI strain. Pangenome analysis indicated the presence of limited accessory genomes with few clades showing specific gene presence/absence pattern. A total of 43 virulence genes were predicted in all the Indian strains of B. melitensis except 2007BM-1 (ricA and wbkA are absent). Multilocus sequence typing (MLST) analysis indicated all except one Indian strain (ADMAS-GI) falling into sequence type (ST 8). In comparison with MLST, core genome phylogeny indicated two major clusters (>70% bootstrap support values) among Indian strains. Clusters with <70% bootstrap support values represent strains with diverse evolutionary origins present among animal and human hosts. Genetic relatedness among animal (sheep and goats) and human strains with 100% bootstrap values shows its zoonotic transfer potentiality. SNP-based analysis indicated similar clustering to that of core genome phylogeny. Among the Indian strains, the highest number of unique SNPs (112 SNPs) were shared by a node that involved three strains from Tamil Nadu. The node SNPs involved several peptidase genes like U32, M16 inactive domain protein, clp protease family protein, and M23 family protein and mostly represented non-synonymous (NS) substitutions. Vaccination has been followed in several parts of the world to prevent small ruminant brucellosis but not in India. Comparison of Indian strains with vaccine strains showed that M5 is genetically closer to most of the Indian strains than Rev.1 strain. The presence of most of the virulence genes among all Indian strains and conserved core genome compositions suggest the use of any circulating strain/genotypes for the development of a vaccine candidate for small ruminant brucellosis in India.

show abstract

Section: Core and Accessory Gene Compositionsmentioning

confidence: 99%

Section: Core and Accessory Gene Compositionsmentioning

confidence: 99%

Genome Sequencing and Comparative Genomics of Indian Isolates of Brucella melitensis

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Brucellosis remains endemic in many parts of the world, including the Middle East, Africa, Latin America, Central Asia, and several regions of the Mediterranean [ 1 , 2 , 3 , 4 ]. Brucella melitensis , Brucella abortus, and Brucella suis are the most important members of the genus because they are responsible for the human disease [ 5 , 6 ] and for significant economic losses in livestock [ 7 , 8 ], while Brucella canis , Brucella neotomae , and Brucella ovis display less or non-zoonotic potential [ 9 ]. Recently, many other species have been described in aquatic mammals (like Brucella pinnipedialis and Brucella ceti ) and other wildlife such as Brucella papionis , Brucella microti , Brucella inopinata, and Brucella vulpis [ 10 , 11 , 12 , 13 , 14 , 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Brucella: Reservoirs and Niches in Animals and Humans

et al. 2021

View full text Add to dashboard Cite

Brucella is an intracellular bacterium that causes abortion, reproduction failure in livestock and leads to a debilitating flu-like illness with serious chronic complications if untreated in humans. As a successful intracellular pathogen, Brucella has developed strategies to avoid recognition by the immune system of the host and promote its survival and replication. In vivo, Brucellae reside mostly within phagocytes and other cells including trophoblasts, where they establish a preferred replicative niche inside the endoplasmic reticulum. This process is central as it gives Brucella the ability to maintain replicating-surviving cycles for long periods of time, even at low bacterial numbers, in its cellular niches. In this review, we propose that Brucella takes advantage of the environment provided by the cellular niches in which it resides to generate reservoirs and disseminate to other organs. We will discuss how the favored cellular niches for Brucella infection in the host give rise to anatomical reservoirs that may lead to chronic infections or persistence in asymptomatic subjects, and which may be considered as a threat for further contamination. A special emphasis will be put on bone marrow, lymph nodes, reproductive and for the first time adipose tissues, as well as wildlife reservoirs.

show abstract

“…The infection in farmed animals usually manifests itself as spontaneous abortions and infertility. The Brucella genus has recently expanded from six to 11 species, all of which are associated with mammals ( Soler-Llorens et al, 2016 ; Suarez-Esquivel et al, 2020 ). Among all Brucella species, B .…”

Section: Introductionmentioning

confidence: 99%

Integrated mRNA-seq and miRNA-seq analysis of goat fibroblasts response to Brucella Melitensis strain M5-90

Li¹,

Chen²,

Wang³

et al. 2021

PeerJ

View full text Add to dashboard Cite

Brucellosis is a globally zoonotic bacterial disease of humans and various animals including goats, sheep, and cattle. Brucella melitensis M5-90, a live attenuated vaccine strain, has been widely used to prevent brucellosis in goats and sheep. However, the molecular mechanisms governing protective immunity response in non-professional phagocytes infected with B. melitensis M5-90 have not been fully investigated, especially in goats. In our research, goat fibroblasts were used as in vitro models to determine these mechanisms by transcriptome analysis. After incubating with B. melitensis M5-90 3 h, the infected goat fibroblasts were collected at 0 h, 4 h, 24 h, 48 h and 72 h for RNA-seq. The results indicated that there were totally 11,819 differentially expressed genes (DEGs) and 777 differentially expressed (DE) miRNAs found in experiment groups compared with the control groups (|log2(Foldchange)|≥1, FDR<0.05). GO and KEGG enrichment analyses revealed that down-regulated genes were involved in the riboflavin metabolism and positive regulation of IL-8 secretion pathway. The up-regulated genes were mainly involved in adaptive immunity, including TNF signaling pathway, MAPK signaling pathway and JAK/STAT pathway. Additionally, cytokine-cytokine receptor interaction, natural killer cell mediated cytotoxicity and toll-like receptor signaling pathway, which associated with innate immunity pathways, were also induced. Based on the Pearson correlation coefficients and prediction results of TargetScan and miRanda, the miRNA-mRNA networks of NFKB1, IFNAR2 and IL10RB were constructed and verified in goat fibroblasts by qPCR, which demonstrated that goat fibroblasts displayed immunomodulatory properties. Our findings provide a deeper insight into the host miRNA-driven B. melitensis defense mechanism and reveal the transcriptome changes involved in the innate and adaptive immune response of the goats to B. melitensis infection.

show abstract

Brucella Genomics: Macro and Micro Evolution

Cited by 44 publications

References 176 publications

Genome Sequencing and Comparative Genomics of Indian Isolates of Brucella melitensis

Genome Sequencing and Comparative Genomics of Indian Isolates of Brucella melitensis

Brucella: Reservoirs and Niches in Animals and Humans

Integrated mRNA-seq and miRNA-seq analysis of goat fibroblasts response to Brucella Melitensis strain M5-90

Contact Info

Product

Resources

About