“…On the assumption that the transient dynamics for both species can be neglected, it is expected that the signal C ( L , t ) will be given by the superposition of two Gaussian-shaped curves with mean values ⟨ t 1 ⟩, ⟨ t 2 ⟩, representing the dimensionless average residence times, and standard deviations σ 1 ( L ) and σ 2 ( L ) , representing the dispersion of residence times about the mean values for the two particle sizes, respectively. The separation resolution, R , associated with this chromatographic experiment can be defined as From eq , R can be expressed in terms of the transport parameters W p eff and D p eff as Thus, the macrotransport approach provides complete information to characterize and interpret the chromatograpic experiment, e.g., by fixing the target resolution value, it allows computation of the minimum column length to achieve the assigned resolution.…”