Brown Trout and European Minnow as Target Species for Genotoxicity Tests: Differential Sensitivity to Heavy Metals

Sanchez-Galan, S.; Linde, A. R.; García‐Vázquez, Eva

doi:10.1006/eesa.1999.1794

Cited by 60 publications

(30 citation statements)

References 18 publications

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“…As a result of the present study Cd and Cu did not significantly induce MN, but significantly induced total nuclear abnormalities at different concentration and exposure times. As one would expect from its essentiality for living organisms, Cu did not induce significant MNi increase in brown trout and European minnow (Phoxinus phoxinus) (Sanchez-Galan et al, 1999)and crucian carp (Carassius auratus gibelio Bloch.) (Arkhipchuk and Garanko, 2005).…”

Section: Discussionmentioning

confidence: 80%

“…Similar results were obtained in other fish species such as Tilapia sp. (Manna and Sadhukhan, 1986;Chandra and Khuda-Bukhsh, 2004), brown trout (Sanchez-Galan et al, 1999) and crucian carp (Arkhipchuk and Garanko, 2005). Different results can be obtained about effects of heavy metals on different species and the contradictory results may be due to different sensitivity of the species after exposure.…”

Section: Discussionmentioning

confidence: 99%

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Güner¹,

Gökalp²

2011

Turk. J. Fish. Aquat. Sci.

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In the present work the induction of micronuclei (MNi) and nuclear abnormalities (NAs) in erythrocytes and Cu and Cd accumulation in whole body of Gambusia affinis were studied. Fish were exposed to two different Cu and Cd concentrations, 0.1 ppm and 1 ppm, for 1 and 2 weeks periods and to Cu-Cd combination (0.1 ppm Cu + 0.1 ppm Cd) for 2 weeks period using a semi-static renewal system. Micronucleus and nuclear abnormality analysis were carried out on peripheral blood erythrocytes. When fishes were exposed Cu and Cd in combination, Cu accumulation was increased compared to their singly (0.1 ppm) exposed concentrations. Micronucleus and nuclear abnormality analysis tests revealed that, although Cu and Cd did not significantly increase micronuclei frequency, nuclear abnormalities were significantly induced compared to control groups.

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Section: Discussionmentioning

confidence: 80%

Section: Discussionmentioning

confidence: 99%

Untitled

Güner¹,

Gökalp²

2011

Turk. J. Fish. Aquat. Sci.

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“…Particularly, the MN test is considered as the most suitable technique in assessing the genotoxic effects of a wide range of compounds in fish (Al-Sabti and Metcalfe 1995;Sanchéz-Galán et al 1999;Rodriguez-Cea et al 2003;Bolognesi et al 2006;Udroiu 2006;Koca et al 2008;Ahmed et al 2011a, b;Bolognesi and Hayashi 2011;Carrola et al 2014). Micronuclei (MNi) are small masses of cytoplasmic chromatin separated from the main cell nucleus, thus are an index of chromosomal breaking or mitotic spindle dysfunctions (Schmid 1975).…”

Section: Introductionmentioning

confidence: 99%

Early genotoxic response and accumulation induced by waterborne copper, lead, and arsenic in European seabass, Dicentrarchus labrax

Canalejo

Díaz-de-Alba

Granado-Castro

et al. 2015

Environ Sci Pollut Res

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Cu, Pb, and As, which are among the most abundant metals in the aquatic environment, are also among the most health-threatened by causing diverse cellular injuries. The aim of this study was to assess and compare the potential early induction of genotoxic effects after waterborne Cu, Pb, and As exposure in European seabass, Dicentrarchus labrax, a commercial widely cultured fish, using the micronucleus (MN) assay in peripheral blood erythrocytes. Fish were exposed under laboratory conditions to nominal solutions ranging 0-10 mg/L for 24 and 96 h. Furthermore, actual metal ion concentrations were measured by inductively coupled plasma atomic emission spectroscopy (ICP-AES) or differential pulse anodic stripping voltammetry (DPASV) in water and four fish tissues differentially related to environmental exposition and metal accumulation, i.e. the gills, liver, muscle, and brain. Dose-dependent increases of micronuclei (MNi) frequency were observed after these very short exposures; based on measured metal concentrations in water, the genotoxic effect ordered as Cu > As > Pb. Significant genotoxic effect at 0.009 mg/L Cu, 0.57 mg/L Pb, and 0.01 mg/L As was seen. For Cu and Pb these are only slightly higher, but for As it is notably lower than the USEPA criteria of maximum concentration to prevent acute toxicity in aquatic organisms.Furthermore, genotoxicity was differentially related to metal accumulation. MNi frequency correlated positively with the content of Pb in all the organs, with the content of As in liver and gills and only with the content of Cu in the brain. In conclusion, our findings raised environmental concerns because these depicted a genotoxic potential of Cu, Pb, and As after a very short exposure to low but environmentally relevant concentrations, too close to regulatory thresholds. In addition, the MN test in D. labrax could be considered an early biomarker of genotoxicity induced by these metals in fish.

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“…Foram encontradas em muitos trabalhos variações na morfologia nuclear de eritrócitos de peixes [28,53,54], que são interpretadas por Ayllon e Garcia-Vasquez [37] como lesões nucleares análogas aos micronúcleos. Alguns autores afirmam que essas anormalidades são induzidas por compostos genotóxicos [28,37,[55][56][57][58][59].…”

Section: Anormalidades Nuclearesunclassified

“…A contaminação dos ecossistemas aquáticos com metais pesados tem chamado à atenção de pesquisadores do mundo todo, que vêm avaliando, constantemente, os efeitos e modo de ação desses contaminantes sobre os organismos vivos [98,99]. [105], O. niloticus [106] e Salmo trutta [54].…”

Section: Ensaio Do Cometaunclassified

Avaliação do potencial genotóxico e mutagênico de amostras de águas de recursos hídricos que recebem efluentes urbanos e industriais do pólo ceramista da cidade de Santa Gertrudes - SP

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Brown Trout and European Minnow as Target Species for Genotoxicity Tests: Differential Sensitivity to Heavy Metals

Cited by 60 publications

References 18 publications

Untitled

Untitled

Early genotoxic response and accumulation induced by waterborne copper, lead, and arsenic in European seabass, Dicentrarchus labrax

Avaliação do potencial genotóxico e mutagênico de amostras de águas de recursos hídricos que recebem efluentes urbanos e industriais do pólo ceramista da cidade de Santa Gertrudes - SP

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