Bronchoalveolar Lavage Fluid Cellular Characteristics, Functional Parameters and Cytokine and Chemokine Levels in Interstitial Lung Diseases

Vašáková, Martina; Šterclová, Martina; Kolesár, Libor; Slavčev, Antonij; Pohunek, Petr; Šulc, Jan; Skibová, J; Striz, Ilja

doi:10.1111/j.1365-3083.2008.02222.x

Cited by 61 publications

(47 citation statements)

References 26 publications

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“…First, we did not observe any angiogenesis in pulmonary sarcoidosis, in contrast with other pulmonary inflammatory fibrotic processes [20]. This absence of angiogenesis is at variance with the expected findings, in light of the high levels of vascular endothelial growth factor in bronchoalveolar lavage fluids reported in sarcoidosis, and in epithelioid cells detected by immunohistochemistry and in situ hybridisation [21,22]. Conversely, macrophages are also known to secrete anti-angiogenic factors, such as endostatin/ collagen XVIII [23].…”

Section: Discussioncontrasting

confidence: 60%

Lymphatic and blood microvasculature organisation in pulmonary sarcoid granulomas

Kambouchner

Pirici²,

Jf³

et al. 2010

Eur Respir J

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Pulmonary sarcoid granulomas are characterised by their elective distribution along collecting lymphatics. However, relationships between granulomas and intralobular lymphatics or blood microvascularisation have not been investigated. Therefore, we undertook a specific analysis of blood capillaries and lymphatics supplying sarcoid granulomas to identify additional clues to understanding the pathophysiogenesis of these lesions.Six pulmonary samples were immunolabelled with D2-40, anti-CD34 and anti-CD31 antibodies, paying particular attention to the relationships between lymphatics and granulomas, and the pattern of blood microvessels supplying sarcoid lesions. A morphometric study of granulomas included their distance to lymphatics and a three-dimensional reconstruction of a granuloma in its lymphatic context. Intralobular granulomas were closely associated with lymphatics; apart from a few granulomas, blood capillaries stopped at the outer border of the fibrous ring surrounding granulomas, and perigranuloma capillaries were particularly scarce.Our observations of the lymphatic and blood microvascular environment of intralobular pulmonary sarcoid granulomas provide evidence for the critical role of lymphatics in the emergence of these lesions. Moreover, pulmonary sarcoid lesions could be considered avascular structures, thereby providing new insights into the understanding of the granuloma physiology and the distribution of blood-borne therapeutic agents.KEYWORDS: Fibrosis, lymphatics, microvessels, sarcoid granulomas S arcoidosis is a multisystem disorder of unknown cause with thoracic involvement in up to 90% of patients [1]. Sarcoid granulomas are comprised of clusters of epithelioid and giant cells surrounded by a rim of lymphocytes and fibroblasts. A peripheral fibrous ring of varied thickness surrounds the lesions that have a tendency to coalesce, resulting in typical fibrotic nodules. The characteristic distribution of granulomas along the pulmonary collecting lymphatics, i.e. peribronchovascular spaces, interlobular septa and subpleural connective tissue, is a major diagnostic criterion [2]. Moreover, their tropism for the outer wall of pulmonary arteries or veins is well known [3].In addition to these features, the relationships between pulmonary sarcoid granulomas and the intralobular lymphatic network or the pulmonary blood capillaries have not yet been adequately examined. Investigating these close relationships might help improve our understanding the biology of granulomas and also orient therapeutic drug research to achieve better targeted distribution. To address this issue, we systematically analysed the interface between granulomas and the lymphatic network by means of D2-40 immunohistochemical labelling or their blood microvessels with CD34 and CD31 immunolabelling [4,5]. To better discriminate their blood and lymphatic vascular supplies we specifically focused our study on selected isolated granulomas, whose location is predominantly intralobular. MATERIALS AND METHODS Surgical lung ...

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Section: Discussioncontrasting

confidence: 60%

Lymphatic and blood microvasculature organisation in pulmonary sarcoid granulomas

Kambouchner

Pirici²,

Jf³

et al. 2010

Eur Respir J

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“…Effects of age and gender on serum CCL2 and CXCL8 concentrations were not significant. In humans, both CXCL8 and CCL2 concentrations were found to be increased in blood (Ziegenhagen et al, 1998a;Suga et al, 1999;Fujiwara et al, 2012) and bronchoalveolar lavage fluid (BALF) (Capelli et al, 2005;Antoniou et al, 2006;Baran et al, 2007) of IPF patients compared with healthy volunteers and correlated with lung function (Capelli et al, 2005;Emad and Emad, 2007;Martina et al, 2009;Vasakova et al, 2009), disease progression (Ziegenhagen et al, 1998b;Totani et al, 2002), and outcome (Shinoda et al, 2009;Richards et al, 2012). Furthermore, several studies suggested an involvement of the chemokine CCL2 in the pathogenesis of IPF, notably through its action on resident pulmonary fibroblast and circulating fibrocytes, promoting the generation of abundant extracellular matrix in the lungs (Gharaee-Kermani et al, 1996;Phillips et al, 2004;Moore et al, 2005;Inomata et al, 2014).…”

Section: Cxcl8 and Ccl2 Concentrationsmentioning

confidence: 99%

Evaluation of chemokines CXCL8 and CCL2, serotonin, and vascular endothelial growth factor serum concentrations in healthy dogs from seven breeds with variable predisposition for canine idiopathic pulmonary fibrosis

Roels

Krafft

Antoine

et al. 2015

Research in Veterinary Science

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The West Highland white terrier (WHWT) is particularly prone to canine idiopathic pulmonary fibrosis (CIPF). We hypothesized that higher circulating concentrations of chemokines CXCL8, CCL2, serotonin (5-HT), or vascular endothelial growth factor (VEGF) could serve as predisposing factors for CIPF development in the WHWT breed. Serum samples from 103 healthy dogs of seven different breeds variably predisposed to CIPF were collected. Serum CXCL8 concentrations were higher in healthy WHWT compared with each of the other groups of healthy dogs. Serum CCL2 concentrations were higher in healthy WHWT and Maltese compared with King Charles spaniels and Malinois Belgian shepherds. No relevant inter-breed differences were observed for serum 5-HT concentrations regarding CIPF predisposition. VEGF values from 89.3% of samples tested were below the kit detection limit. In conclusion, high CXCL8 blood concentrations and possibly CCL2 concentrations might be related to the breed predisposition of the WHWT for CIPF and warrants further investigations.

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“…One hypothesis is pathogenic cytokine release. Tumor necrosis factor-α(TNF-α) and interleukins are thought to be the dominating contributing cytokines [9,10]. Recently there was case reported that Nod-like receptor pyrin domaincontaining protein 3(NLRP3)may act as an initiator of inflammation process in lung of R-ILD patient.…”

Section: Discussionmentioning

confidence: 99%

Interstitial pneumonitis during rituximab-containing chemotherapy for primary central nervous system lymphomas: a case report and review of literature

Sun

Liu

et al. 2018

Chin Neurosurg Jl

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Background: Primary central nervous system lymphoma(PCNSL) is a rare kind of non-Hodgkin lymphoma. Rituximab combined with high-dose methotrexate, cytarabine and dexamethasone (R-MAD regimen) were reported effective for PCNSL patients. Rituximab can cause several side effects, including fever, chills and rigors. Case presentation: In this case report, we demonstrate rituximab-induced interstitial pneumonitis in a PCNSL patient who has been treated with R-MAD regimen. The patient recovered after treatment and she remains complete remission after following consolidation chemotherapy. Conclusions: Here is no report of potential fatal complications of Rituximab like interstitial pneumonitis nowadays in PCNSL patients. As Rituximab is widely used, physicians should raise their awareness of this rare complication and detect RTX-ILD in early stage.

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Bronchoalveolar Lavage Fluid Cellular Characteristics, Functional Parameters and Cytokine and Chemokine Levels in Interstitial Lung Diseases

Cited by 61 publications

References 26 publications

Lymphatic and blood microvasculature organisation in pulmonary sarcoid granulomas

Lymphatic and blood microvasculature organisation in pulmonary sarcoid granulomas

Evaluation of chemokines CXCL8 and CCL2, serotonin, and vascular endothelial growth factor serum concentrations in healthy dogs from seven breeds with variable predisposition for canine idiopathic pulmonary fibrosis

Interstitial pneumonitis during rituximab-containing chemotherapy for primary central nervous system lymphomas: a case report and review of literature

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