Bronchial Microbiome, PA Biofilm-Forming Capacity and Exacerbation in Severe COPD Patients Colonized by
            <i>P. Aeruginosa</i>

García-Núñez, Marian; Martí, Sara; Puig, Carmen; Brocal, Vicente Pérez; Millares, Laura; Santos, Salud; Ardanuy, Carmen; Moya, Andrés; Liñares, Josefina; Monsó, Eduard

doi:10.2217/fmb-2016-0127

Cited by 28 publications

(25 citation statements)

References 50 publications

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“…Wu et al showed a significant increase in abundance of Streptococcus pneumoniae, Klebsiella pneumoniae , and Pseudomonas aeruginosa in COPD as compared to healthy ( 51 ). Recently, this was further confirmed by Garcia-Nuñez et al that potentially pathogenic microorganisms other than P. aeruginosa can be the cause of exacerbations in COPD, and hence antibiotic treatments against the activity of other pathogens need to be considered while treating COPD ( 52 ).…”

Section: Microbiome In Copdmentioning

confidence: 84%

Potential of Health and Demographic Surveillance System in Asthma and Chronic Obstructive Pulmonary Disease Microbiome Research

Dhotre

Patil

Shouche

et al. 2017

Front. Public Health

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Health and demographic surveillance system (HDSS) is a population-based health and vital event registration system that monitors demographic and health events in a geographically defined population at regular intervals. Human microbiome research in the past decade has been the field of increasingly intense research much due to its demonstrated impact upon various health conditions including human chronic airway diseases such as asthma and chronic obstructive pulmonary disease (COPD). Many confounding factors have been revealed to play a role in shaping the microbiome in chronic airway diseases. Asthma and COPD follows a typical pattern of disease progression, which includes stable and exacerbation state in which the microbiota is known to vary. However, many such studies lack extensive and longitudinal sampling with inadequate metadata, which has resulted in the inconsistencies in the observations. HDSS provides such a platform, which can offer a deeper understanding of the role of the microbiome in human health. In this review, we highlight opportunities and limitations in microbiome research with the help of studies conducted on chronic airway diseases like asthma and COPD. In addition, we also emphasize on the benefits of HDSS and future directions in lung microbiome research.

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Section: Microbiome In Copdmentioning

confidence: 84%

Potential of Health and Demographic Surveillance System in Asthma and Chronic Obstructive Pulmonary Disease Microbiome Research

Dhotre

Patil

Shouche

et al. 2017

Front. Public Health

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“…Thus, the examination of the respiratory microbiome confirms that a causal bacterial pathogen may keep unnoticed by conventional microbiology in some exacerbations, while the culture identifies microorganisms that are only colonizers. This equivocal information of conventional microbiology has been reported in up to twenty percent of patients chronically colonized by Pseudomonas aeruginosa (4,27).…”

Section: Exacerbationmentioning

confidence: 99%

“…An OTU will be considered to be equivalent to this reference at gender level when the sequence is at least 94% coincident, and to species when this coincidence is over 97%. The composition of the microbiome is in most cases expressed as relative abundance, meaning the proportion of copies of the 16S rRNA gene corresponding to each identified OTU from the whole, a value that has shown to be correlated with absolute microbial counts recovered at culture, at least for Pseudomonas aeruginosa (4). 16S rRNA gene analysis is unable to obtain virus and fungal information, however, and these microorganisms need to be targeted with other sequencing approaches.…”

Section: The Respiratory Microbiomementioning

confidence: 99%

“…Different studies have demonstrated that bacteria clearly overrepresented in analyses of bronchial secretions may not be recovered by culture, while colonizing microorganisms grow easily from the analyzed sample, in spite that their relative abundance have not changed from previous stability. This situation has been described mainly for patients showing chronic colonization by Pseudomonas aeruginosa, who suffer from exacerbations that in most cases are due to other PPMs, in spite of the persistence of positive cultures for Pseudomonas (4,29). Thus, the examination of the respiratory microbiome confirms that a causal bacterial pathogen may keep unnoticed by conventional microbiology in some exacerbations, while the culture identifies microorganisms that are only colonizers.…”

Section: Exacerbationmentioning

confidence: 99%

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Microbiome in chronic obstructive pulmonary disease

Monsó¹

2017

Ann. Transl. Med.

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The introduction of culture-independent techniques for the microbiological analysis of respiratory samples has confirmed that the respiratory system hosts a large number of microorganisms, which include a wide range of bacteria. The regular exposure to tobacco smoke changes the microbiome in healthy smokers, first in the oropharynx, increasing the presence of a restricted number of genera which attain high relative abundance, a pattern that may be considered as dysbiosis. In chronic obstructive pulmonary disease (COPD), microbiome analyses of sputum samples have demonstrated an important decline in bacterial diversity, with a change to a restricted flora with an overrepresentation of the Proteobacteria phylum, which include most of the bacteria commonly considered as potentially pathogenic microorganisms, paralleled by a decline in the relative abundance of microorganisms part of the Firmicutes phylum. In exacerbations, specific bacteria overrepresented in microbiome analyses and potentially causal of the acute episode may not be recovered by sputum culture, while colonizing microorganisms grow easily, in spite that their relative abundance have not changed from previous stability. This situation has been described in patients showing chronic colonization by Pseudomonas aeruginosa, who suffer from exacerbations that in most cases are due to other PPMs, in spite of the persistence of positive cultures for the colonizing Pseudomonas strains.Interaction between different microorganisms can be addressed through microbiome analyses, and functional metagenomics, that describes the genomic potential of the community, has shown that, in spite that the bronchial microbiome as a whole may not change significantly, clear changes in carbohydrate metabolism, cancer, cell growth and death, transport and catabolism pathways often appear during exacerbations. These functional changes may be important because through them the resident community as a whole show its power to modify important metabolic patterns.

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“…Узагальнення наукових публікацій останніх років показує, що перебування мікроорганізмів у дихальних шляхах є складовою патогенезу ХОЗЛ і, навіть, поза стадії загострення, вони приймають участь у прогресуванні ХОЗЛ, в більшості випадків, через активацію ефекторних клітин запалення [15,16,19,20,23,24].…”

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Microbiota of the Bronchopulmonary Apparatus in the Exacerbation of the Obstructive Pulmonary Disease of Professional Etiology

Ivchuk¹,

Kovalchuk²

2018

Ukr. ž. med. bìol. sportu

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Встановлено, що за перебігу хронічного обструктивного захворювання легень професійної етіології, серед виділеної мікробіоти є грампозитивні (48,9%), грамнегативні (27,6%) мікроорганізми та дріжджоподібні гриби (23,5%). Значну питому вагу мали штами Staphylococcus aureus (26,7%), Candida albicans (22,3%), Klebsiella pneumoniae (10,9%), Escherichia coli (9,3%), Streptococcus viridans (5,7%) та Staphylococcus epidermidis (5,5%). У структурі видового складу мікроорганізмів виділених з мокротиння хворих на хронічне обструктивне захворювання легень професійної етіології, залежно від стадії перебігу захворювання переважали: S. aureus (29,2%), K. pneumoniae (12,5%) та E. coli (9,7%)-за хронічного обструктивного захворювання легень 1 стадії; S. aureus (31,8%), E. coli (11,9%) та K. pneumoniae (10,6%)-за хронічного обструктивного захворювання легень 2 стадії; S. aureus (18,8%), K. pneumoniae (9,7%) та S. epidermidis (9,7%)-за хронічного обструктивного захворювання легень 3 стадії. Ключові слова: хронічне обструктивне захворювання легень, мікробіоценоз повітроносних шляхів, гірничодобувна промисловість.

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Bronchial Microbiome, PA Biofilm-Forming Capacity and Exacerbation in Severe COPD Patients Colonized by P. Aeruginosa

Abstract: The bronchial microbiome shows differences according with P. aeruginosa biofilm-forming capacity. Pathogenic microorganisms other than P. aeruginosa cause a significant part of the exacerbations in colonized chronic obstructive pulmonary disease patients.

Cited by 28 publications

References 50 publications

Potential of Health and Demographic Surveillance System in Asthma and Chronic Obstructive Pulmonary Disease Microbiome Research

Potential of Health and Demographic Surveillance System in Asthma and Chronic Obstructive Pulmonary Disease Microbiome Research

Microbiome in chronic obstructive pulmonary disease

Microbiota of the Bronchopulmonary Apparatus in the Exacerbation of the Obstructive Pulmonary Disease of Professional Etiology

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