Bromine Analysis in 5-Bromouracil-Labeled DNA by X-ray Fluorescence

Abstract

The degree of replacement of thymine by 5-bromouracil in the deoxyribonucleic acid (DNA) of the thymine-deficient bacterium Escherichia coli B 15 T(-) has been determined by a nondispersive x-ray fluorescence method. A measure of the fraction by weight of bromine in the purified DNA is required for determining the degree of thymine replacement. This fraction is experimentally determined on 35-microg samples of DNA by x-ray spectroanalysis.

Synchrotron X-ray fluorescence studies of a bromine-labelled cyclic RGD peptide interacting with individual tumor cells

“…in blood serum) (Lyon et al, 2005;Underwood, 1971) and is generally nontoxic when bound to organic molecules. Bromine has been used previously as a heavy-atom label for X-ray crystallographic studies involving parasymphatolytic drug production and DNA labelling (Paradellis & Skouras, 1979;Zeitz & Lee, 1963).…”