Brominated Furanones Inhibit Biofilm Formation by
            <i>Salmonella enterica</i>
            Serovar Typhimurium

Janssens, J.; Steenackers, Hans; Robijns, Stijn; Gellens, Edith; Levin, Jeremy; Zhao, Hui; Hermans, Kim; Coster, David De; Verhoeven, Tine; Marchal, Kathleen; Vanderleyden, Jos; Vos, Dirk De; Keersmaecker, Sigrid C. J. De

doi:10.1128/aem.01262-08

Cited by 194 publications

(167 citation statements)

References 67 publications

(83 reference statements)

Supporting

Mentioning

153

Contrasting

Unclassified

Order By: Relevance

“…Food contamination may occur at any stage of the production process and can result in contamination of the processing environment (Simões et al 2010, Srey et al 2013. It has been recognized that biofilms are a frequent source for infections and almost 80% of persistent bacterial infections in the United States were found to be associated with biofilms (Janssens et al 2008). …”

Section: Discussionmentioning

confidence: 99%

Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis

Costa

Rossatto

Medeiros

et al. 2018

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

The adhesion ability of bacteria to abiotic surfaces has important implications in food industries, because these organisms can survive for long periods through the biofilm formation. They can be transferred from one place to another in the industry causing contamination of the food processing environment. In this study, the antibacterial and antibiofilm activities of the antimicrobial peptide P34, characterized as a bacteriocinlike substance (BLS P34) were tested against planktonic and sessile cells of Staphylococcus aureus and Enterococcus faecalis isolated from foods. The BLS P34 showed inhibitory effect against all planktonic cells of E. faecalis. The inhibition of biofilm formation and the eradication of pre-formed biofilm were evaluated with the crystal violet assay and with the reduction of 3-bromide [4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium. The BLS P34 promoted a reduction of percentage of adhered microbial cells on the surface, not being able to perform the complete elimination of biofilm formation. The metabolic activity of S. aureus biofilms decreased considerably between 41-95%. However, E. faecalis cells showed up metabolically stimulated. The BLS P34 has the potential antibiofilm for the species S. aureus. Studies suggest more detailed approaches to a better understanding of the interactions between the antimicrobial and bacterial cells within the biofilm structure.

show abstract

Section: Discussionmentioning

confidence: 99%

Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis

Costa

Rossatto

Medeiros

et al. 2018

An. Acad. Bras. Ciênc.

View full text Add to dashboard Cite

show abstract

“…As cepas foram avaliadas quanto à capacidade de produção de biofilme em placas de microtitulação de 96 poços (Nunclon, Nune, Roskilde, Denmark), seguindo a técnica descrita por Janssens et al (20) , com modificações, de forma a adaptar o método para V. parahaemolyticus. Foram colocados 200 µL de caldo APA-3% NaCl em cada poço da placa de microtitulação adicionados de 2 µL de culturas overnight em APA-3% NaCl de cada cepa padronizada em espectrofotômetro a 600 nm para valor 0,5 de densidade ótica (DO), o que corresponde a aproximadamente 5,5.10 6 UFC/mL.…”

Section: Materiais E Métodosunclassified

FORMAÇÃO DE BIOFILME APÓS ESTRESSE SUBLETAL POR Vibrio parahaemolyticus ISOLADOS DE CAMARÃO-ROSA (Farfantepenaeus paulensis)

et al. 2017

View full text Add to dashboard Cite

ResumoO consumo de frutos do mar, como o camarão-rosa, contaminados com V. parahaemolyticus pode causar gastrenterite aguda. O objetivo deste trabalho foi verificar a capacidade de V. parahaemolyticus isolados de camarões provenientes do estuário da Lagoa dos Patos formarem biofilme após exposição a diferentes tipos de estresse subletal. Para obtenção das amostras, foram realizadas 12 coletas de Farfantepenaeus paulensis, durante o período de safra de 2016. As amostras foram analisadas quanto à presença de V. parahaemolyticus. As cepas foram avaliadas quanto à capacidade de produção de biofilme em placas de microtitulação, tanto antes como após serem submetidas a diferentes tipos de estresse subletal. V. parahaemolyticus foi isolado de 16,66% (2/12) das amostras. Uma das duas cepas isoladas foi capaz de formar biofilme, sendo classificada como fraca formadora de biofilme, a outra foi classificada como não formadora de biofilme. Nenhuma das cepas alterou sua capacidade de formar biofilme quando submetida aos estresses de 4 ºC e 20 ºC; contudo, com relação aos estresses de 42 ºC e pH ácido, cada cepa respondeu de uma maneira diferente. Palavras-chave: camarão-rosa; contaminação; doenças transmitidas por alimentos. AbstractConsumption of seafood, such as pink shrimp, contaminated by V. parahaemolyticus can cause acute gastroenteritis. The objective this study was to verify the ability of V. parahaemolyticus isolated from shrimp from Lagoa dos Patos estuary to form biofilm after exposure to different types of sublethal stress. To obtain the samples, 12 collections of Farfantepenaeus paulensis were carried out during the 2016 harvest period. The samples were analyzed for the presence of V. parahaemolyticus. The strains were evaluated for biofilm production capacity on microtiter plates, both before and after being submitted to different types of sublethal stress. V. parahaemolyticus was isolated from 16.66% (2/12)

show abstract

“…In immunocompromised people, Salmonella infections are often fatal if they are not treated promptly with antibiotics (Janssens et al, 2008). While Salmonella infections are most commonly treated using fluoroquinolones (e.g., ciprofloxacin) and extended spectrum cephalosporins (e.g., cefotaxime), there are disturbing reports regarding the development of resistance against these antimicrobials.…”

Section: Salmonella Biofilm Control Measuresmentioning

confidence: 99%

“…While Salmonella infections are most commonly treated using fluoroquinolones (e.g., ciprofloxacin) and extended spectrum cephalosporins (e.g., cefotaxime), there are disturbing reports regarding the development of resistance against these antimicrobials. Further, Salmonella is able to form biofilms on a variety of biotic and abiotic surfaces, where they are a double threat in that they allow the Salmonella to survive and spread in the environment outside the host (Janssens et al, 2008). The Salmonella found in these biofilms show an even higher tolerance to antibiotics than most Salmonella and according to the National Institutes of Health; approximately 80% of persistent bacterial infections in the United States are caused by biofilms (NIH, 1997).…”

Section: Salmonella Biofilm Control Measuresmentioning

confidence: 99%

See 1 more Smart Citation

Invasion and Survival of Salmonella in the Environment: The Role of Biofilms

Sheffield¹,

Crippen²

2012

Salmonella - A Diversified Superbug

View full text Add to dashboard Cite

Brominated Furanones Inhibit Biofilm Formation by Salmonella enterica Serovar Typhimurium

Cited by 194 publications

References 67 publications

Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis

Evaluation antibacterial and antibiofilm activity of the antimicrobial peptide P34 against Staphylococcus aureus and Enterococcus faecalis

FORMAÇÃO DE BIOFILME APÓS ESTRESSE SUBLETAL POR Vibrio parahaemolyticus ISOLADOS DE CAMARÃO-ROSA (Farfantepenaeus paulensis)

Invasion and Survival of Salmonella in the Environment: The Role of Biofilms

Contact Info

Product

Resources

About