Bromide space determination using anion-exchange chromatography for measurement of bromide

Abstract

A high-pressure liquid chromatographic method for bromide measurement is used to determine extracellular water volume in humans. The method uses 5 microL serum ultrafiltrate and has a sensitivity of 7.5 pmol. Because of the extreme sensitivity of this method, relatively small quantities of Br can be administered and small amounts of blood are needed for the analysis. By this method, the mean corrected Br space in 82 healthy adults representing a wide range of body weights was 0.218 +/- .034 L/kg (mean +/- 1 SD) with a range of 0.153-0.295 L/kg, which is consistent with reported values. There was a significant, inverse relationship between corrected Br space per kilogram and obesity as measured by body mass index. The corrected Br space in six children aged 3-36 mo was 0.335-0.394 L/kg, which is also consistent with reported values in children of this age. This method for Br measurement can easily and readily be applied for the determination of extracellular water volume.

Utility of Standard Nutritional Parameters in Detecting Body Cell Mass Depletion in Patients With End-Stage Liver Disease

“…BCM was measured using isotope dilution with correction for extracellular water, measured by bromide dilution as previously described. [19][20][21] BCM was calculated based on the assumption that intracellular water constitutes 73.2% of BCM, 22 and calculation of intracellular water was based on the difference between total body water and extracellular water. Total body water was determined using H 2 18 O as a tracer (Cambridge Isotopes, Cambridge, MA), described by Schoeller et al 19 Extracellular water was determined through bromide (Sigma Chemical Corp, St Louis, MO) dilution, described previously by Miller et al 20 BCM values were normalized for height.…”

Body composition in patients with an ileostomy and inflammatory bowel disease: validation of bio-electric impedance spectroscopy (BIS)

“…ECW (DIL) was determined as bromide space, modified after Miller et al, (1989). After 3 h of equilibration, a blood sample was drawn, serum was separated and stored at 7 20 C until analysis.…”

“…After 3 h of equilibration, a blood sample was drawn, serum was separated and stored at 7 20 C until analysis. Samples were deproteinised using methanol and after subsequent centrifugation, bromide concentration was determined by high performance ion chromatography (HPIC, Dionex ion-chromatographic The correction factor for non-extracellular distribution of Br ¼ 0.90, and correction for the Donnan equilibrium ¼ 0.95 (Miller et al, 1989).…”

Body composition in Prader-Willi syndrome compared with nonsyndromal obesity: Relationship to physical activity and growth hormone function

“…The ECW compartment was determined by bromide dilution, and the bromide concentration was determined from serum after a 10-hour fast. Corrected bromide space was calculated according to the method of Miller et al 29 ICW is calculated by subtracting ECW from TBW. …”