Bromelain exerts anti-inflammatory effects in an ovalbumin-induced murine model of allergic airway disease

Secor, Eric R.; Carson, William F.; Cloutier, Michelle M.; Guernsey, Linda; Schramm, Craig M.; Wu, Carol A.; Thrall, Roger S.

doi:10.1016/j.cellimm.2005.10.002

Cited by 71 publications

(62 citation statements)

References 39 publications

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“…The level of proof, method of bromelain administration and dose, and quality of the studies vary, but beneficial effects were suggested or proven in a variety of inflammatory diseases and models of inflammation. These include the experimental allergic encephalomyelitis (EAE) model of the human autoimmune disease multiple sclerosis (3), carrageenan-induced pleurisy in the rat (4)(5)(6), immunologically mediated arteriosclerosis in rat aortic allografts (7), rheumatologic diseases in mice and humans (8)(9)(10)(11)(12)(13), and allergic asthma (14) and rhinitis (15). Some studies demonstrated that bromelain had efficacy similar to standard anti-inflammatory drugs such as dexamethasone (5,6) or non-steroidal anti-inflammatory agents (NSAIDs) (8,10,11,16).…”

Section: Introductionmentioning

confidence: 99%

Bromelain treatment decreases neutrophil migration to sites of inflammation

Fitzhugh

Shan

Dewhirst

et al. 2008

Clinical Immunology

108

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Bromelain, a mixture of proteases derived from pineapple stem, has been reported to have therapeutic benefits in a variety of inflammatory diseases, including murine inflammatory bowel disease. The purpose of this work was to understand potential mechanisms for this anti-inflammatory activity. Exposure to bromelain in vitro has been shown to remove a number of cell surface molecules that are vital to leukocyte trafficking, including CD128a/CXCR1 and CD128b/CXCR2 that serve as receptors for the neutrophil chemoattractant IL-8 and its murine homologues. We hypothesized that specific proteolytic removal of CD128 molecules by bromelain would inhibit neutrophil migration to IL-8 and thus decrease acute responses to inflammatory stimuli. Using an in vitro chemotaxis assay, we demonstrated a 40% reduction in migration of bromelain-vs. sham-treated human neutrophils in response to rhIL-8. Migration to the bacterial peptide analog fMLP was unaffected, indicating that bromelain does not induce a global defect in leukocyte migration. In vivo bromelain treatment generated a 50 -85% reduction in neutrophil migration in 3 different murine models of leukocyte migration into the inflamed peritoneal cavity. Intravital microscopy demonstrated that although in vivo bromelain treatment transiently decreased leukocyte rolling, its primary long-term effect was abrogation of firm adhesion of leukocytes to blood vessels at the site of inflammation. These changes in adhesion were correlated with rapid re-expression of the bromelain-sensitive CD62L/L-selectin molecules that mediate rolling following in vivo bromelain treatment and minimal re-expression of CD128 over the time period studied. Taken together, these studies demonstrate that bromelain can effectively decrease neutrophil migration to sites of acute inflammation and support the specific removal of the CD128 chemokine receptor as a potential mechanism of action.

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Section: Introductionmentioning

confidence: 99%

Bromelain treatment decreases neutrophil migration to sites of inflammation

Fitzhugh

Shan

Dewhirst

et al. 2008

Clinical Immunology

108

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“…Bromelain is an efficient therapeutic agent and is being used successfully in allergic airway disease (AAD) (Secor et al 2012). Bromelain treats asthma by decreasing the level of total BAL leukocytes (eosinophils and lymphocytes) and cellular infiltrates (Secor et al 2005) and also notably lessens BAL CD4+, CD8+ T CD4+ and CD25+T cells (Jaber 2002;Darshan and Doreswamy 2004). It also reduces interleukins IL-4, IL-12, IL-13, IL-17 and IFN-α in the serum as well as changes the proportion of CD4+/CD8+ (Secor et al 2005a;2007b;2012c;2013d).…”

Section: Asthmamentioning

confidence: 99%

Bromelain: Methods of Extraction, Purification and Therapeutic Applications

Manzoor

Nawaz

Mukhtar

et al. 2016

Braz. arch. biol. technol.

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“…It reduces the total numbers of leukocytes, eosinophils, CD4 + and CD8 + T cells in BALF, and decreases IL-13 concentration, which is a critical mediator for AHR in asthma [266]. In separate study, oral supplementation has been shown to suppress airway methacholine sensitivity, decrease IL-13 level, and eosinophils, CD19 + B cells and CD8 + T cells counts in BAL [267].…”

Section: Bromelainmentioning

confidence: 99%