Bromelain and Nisin: The Natural Antimicrobials with High Potential in Biomedicine

Jančič, Urška; Gorgieva, Selestina

doi:10.3390/pharmaceutics14010076

Cited by 34 publications

(27 citation statements)

References 210 publications

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“…At high nisin concentrations, the signal that induces the promoter activity is strong, and as a result, an increase in target protein expression can be expected [ 25 ]. However, because nisin exhibits antibacterial activity due to pore formation in the bacterial cell wall [ 30 ], it was necessary in our study to determine the optimal nisin concentration for efficient CC12.1scFv expression. Nisin was added at various concentrations to induce gene expression.…”

Section: Discussionmentioning

confidence: 99%

Development of a Single-Chain Fragment Variable that Binds to the SARS-CoV-2 Spike Protein Produced by Genetically Modified Lactic Acid Bacteria

et al. 2023

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SARS-CoV-2 enters cells via binding of the surface-exposed spike protein RBD to host cell ACE2 receptors. Therefore, in this study, we designed a scFv (single-chain fragment variable) based on the amino acid sequence of CC12.1, a neutralizing antibody found in the serum of patients with COVID-19. scFv is a low-molecular-weight antibody designed based on the antibody-antigen recognition site. Compared with the original antibody, scFv has the advantages of high tissue penetration and low production cost. In this study, we constructed gmLAB (genetically modified lactic acid bacteria) by incorporating the designed scFv into a gene expression vector and introducing it into lactic acid bacteria, aiming to develop microbial therapeutics against COVID-19. In addition, gmLAB were also constructed to produce GFP-fused scFv as a means of visualizing scFv. Expression of each scFv was confirmed by Western blotting, and the ability to bind to the RBD was investigated by ELISA. This study is the first to design a scFv against the RBD of SARS-CoV-2 using gmLAB and could be applied in the future.

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Section: Discussionmentioning

confidence: 99%

Development of a Single-Chain Fragment Variable that Binds to the SARS-CoV-2 Spike Protein Produced by Genetically Modified Lactic Acid Bacteria

et al. 2023

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“…One of the problems with using enzymes (like bromelain) is that their activity decreases after processing or over time [32]. Immobilization is a potential method for ensuring their efficiency [33].…”

Section: Immobilization Of Bromelainmentioning

confidence: 99%

Bromelain: A Potent Phytomedicine

Agrawal¹,

Nikhade²,

Patel³

et al. 2022

Cureus

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The commercially available protein-digesting enzyme bromelain is derived from the pineapple fruit or stem. Bromelain from fruit and stems are produced in different ways and has varied enzyme compositions. "Bromelain" often refers to the "stem bromelain". Bromelain is a combination of several thiol endopeptidases and others including various protease inhibitors, glucosidase, cellulase, phosphatase, peroxidase, and escharase. Studies conducted in both the lab and on animals show that bromelain has a variety of fibrinolytic, anti-edematous, antithrombotic, and anti-inflammatory effects. The body can absorb bromelain to a significant extent without it ceasing its proteolytic activity or having any negative side effects. Numerous therapeutic advantages of bromelain include wound debridement, improved drug absorption, and the management of sinusitis, bronchitis, angina pectoris, surgical trauma, and thrombophlebitis. Additionally, it treats numerous cardiovascular conditions, diarrhoea, and osteoarthritis. Bromelain also encourages apoptotic cell death and exhibits some anti-cancerous properties. This review compiles the crucial traits, medical and dental uses of bromelain as well as its potential mechanism of action.

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“…The protein bromelain purified from the stem is made in a different way and has a different enzymatic makeup, such as peroxidase and cellulase, and enzyme inhibitors are included. The therapeutic potential of bromelain has been reported, with it having antiedematous, fibrinolytic, antithrombotic, antibacterial, and anti-inflammatory properties in both in vitro and in vivo studies [ 1 , 2 , 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…Vaccines against SARS-CoV-2 have been developed by Moderna, BioNTech/Pfizer, Janssen, AstraZeneca, SinoVac, and Gamaleya pharmaceuticals to manage the disease [ 2 , 3 ]. However, the vast worldwide population of concern is still far behind in receiving vaccinations.…”

Section: Introductionmentioning

confidence: 99%

Exploring the Binding Interaction of Active Compound of Pineapple against Foodborne Bacteria and Novel Coronavirus (SARS-CoV-2) Based on Molecular Docking and Simulation Studies

et al. 2022

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Natural resources, particularly plants and microbes, are an excellent source of bioactive molecules. Bromelain, a complex enzyme mixture found in pineapples, has numerous pharmacological applications. In a search for therapeutic molecules, we conducted an in silico study on natural phyto-constituent bromelain, targeting pathogenic bacteria and viral proteases. Docking studies revealed that bromelain strongly bound to food-borne bacterial pathogens and SARS-CoV-2 virus targets, with a high binding energy of −9.37 kcal/mol. The binding interaction was mediated by the involvement of hydrogen bonds, and some hydrophobic interactions stabilized the complex and molecular dynamics. Simulation studies also indicated the stable binding between bromelain and SARS-CoV-2 protease as well as with bacterial targets which are essential for DNA and protein synthesis and are required to maintain the integrity of membranous proteins. From this in silico study, it is also concluded that bromelain could be an effective molecule to control foodborne pathogen toxicity and COVID-19. So, eating pineapple during an infection could help to interfere with the pathogen attaching and help prevent the virus from getting into the host cell. Further, research on the bromelain molecule could be helpful for the management of COVID-19 disease as well as other bacterial-mediated diseases. Thus, the antibacterial and anti-SARS-CoV-2 virus inhibitory potentials of bromelain could be helpful in the management of viral infections and subsequent bacterial infections in COVID-19 patients.

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Bromelain and Nisin: The Natural Antimicrobials with High Potential in Biomedicine

Cited by 34 publications

References 210 publications

Development of a Single-Chain Fragment Variable that Binds to the SARS-CoV-2 Spike Protein Produced by Genetically Modified Lactic Acid Bacteria

Development of a Single-Chain Fragment Variable that Binds to the SARS-CoV-2 Spike Protein Produced by Genetically Modified Lactic Acid Bacteria

Bromelain: A Potent Phytomedicine

Exploring the Binding Interaction of Active Compound of Pineapple against Foodborne Bacteria and Novel Coronavirus (SARS-CoV-2) Based on Molecular Docking and Simulation Studies

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