Bromcresol purple method for serum albumin gives falsely low values in patients with renal insufficiency

Maguire, Gerald A.; Price, Christopher P.

doi:10.1016/0009-8981(86)90102-6

Cited by 36 publications

(11 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This variability is insufficient to significantly alter 5vMELD scores. However, since the BCP method may underestimate albumin in patients on hemodialysis [40] and with conjugated hyperbilirubinemia [41], the impact of different albumin assay methods and the inter-laboratory variability of 5vMELD should be investigated. Importantly, similar concerns have been raised regarding the components of MELD, particularly serum creatinine and INR, leading some to advocate standardization of these assays by UNOS [42], [43], [44].…”

Section: Discussionmentioning

confidence: 99%

Revision of MELD to Include Serum Albumin Improves Prediction of Mortality on the Liver Transplant Waiting List

et al. 2013

View full text Add to dashboard Cite

BackgroundAllocation of donor livers for transplantation in most regions is based on the Model for End-Stage Liver Disease (MELD) or MELD-sodium (MELDNa). Our objective was to assess revisions to MELD and MELDNa that include serum albumin for predicting waiting list mortality.MethodsAdults registered for liver transplantation in the United States (2002–2007) were identified from the United Network for Organ Sharing (UNOS) database. Cox regression was used to determine the association between serum albumin and 3-month mortality, and to derive revised MELD and MELDNa scores incorporating albumin (‘MELD-albumin’ and ‘5-variable MELD [5vMELD]’).ResultsAmong 40,393 patients, 9% died and 24% underwent transplantation within 3 months of listing. For serum albumin concentrations between 1.0 and 4.0 g/dL, a linear, inverse relationship was observed between albumin and 3-month mortality (adjusted hazard ratio per 1 g/dL reduction in albumin: 1.44; 95% CI 1.35–1.54). The c-statistics for 3-month mortality of MELD-albumin and MELD were 0.913 and 0.896, respectively (P<0.001); 5vMELD was superior to MELDNa (c-statistics 0.922 vs. 0.912, P<0.001). The potential benefit of 5vMELD was greatest in patients with low MELD (<15). Among low MELD patients who died, 27% would have gained ≥10 points with 5vMELD over MELD versus only 4–7% among low MELD survivors and high MELD (≥15) candidates (P<0.0005).ConclusionModification of MELD and MELDNa to include serum albumin is associated with improved prediction of waiting list mortality. If validated and shown to be associated with reduced mortality, adoption of 5vMELD as the basis for liver allograft allocation may improve outcomes on the liver transplant waiting list.

show abstract

Section: Discussionmentioning

confidence: 99%

Revision of MELD to Include Serum Albumin Improves Prediction of Mortality on the Liver Transplant Waiting List

et al. 2013

View full text Add to dashboard Cite

show abstract

“…17,18 Bromcresol purple, on the other hand, underestimates albumin concentrations in people with renal failure or increased serum bilirubin concentration. 19,20 Other molecules, such as heparin, also can interfere with albumin assays, an effect that is less pronounced with bromcresol purple. 21 Although bovine serum albumin has better affinity for bromcresol green than does human serum albumin, reaction time and the animal's age may affect serum albumin levels, the latter pre- sumably caused by changing globulin patterns during development.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of variation in serum globulin concentrations in dairy cattle

Chorfi

Lanevschi-Pietersma

Girard

et al. 2004

Veterinary Clinical Pathol

View full text Add to dashboard Cite

show abstract

“…Therefore, accurate measurement of albumin is critical in monitoring patients on dialysis. In non-renal patients, the BCP method gives albumin results in good agreement with the immunological methods, while in patients on haemodialysis the BCP method underestimates the serum albumin (6,7). Due to the cost of the immunochemical methods, albumin measurements are usually performed using either bromcresol green (BCG) or bromcresol purple (BCP) methods by clinical laboratories.…”

Section: Introductionmentioning

confidence: 99%

“…Immunochemical quantitation by nephelometry is considered the gold standard for the measurement of serum albumin. Therefore, BCP is widely used to estimate albumin levels in nonrenal patients (7,8). Systemic differences between the BCP and the BCG methods have been well studied (3)(4)(5).…”

Section: Introductionmentioning

confidence: 99%

Lithium heparinised blood-collection tubes give falsely low albumin results with an automated bromcresol green method in haemodialysis patients

Meng

Krahn

2008

Clinical Chemical Laboratory Medicine

View full text Add to dashboard Cite

show abstract

Bromcresol purple method for serum albumin gives falsely low values in patients with renal insufficiency

Cited by 36 publications

References 10 publications

Revision of MELD to Include Serum Albumin Improves Prediction of Mortality on the Liver Transplant Waiting List

Revision of MELD to Include Serum Albumin Improves Prediction of Mortality on the Liver Transplant Waiting List

Evaluation of variation in serum globulin concentrations in dairy cattle

Lithium heparinised blood-collection tubes give falsely low albumin results with an automated bromcresol green method in haemodialysis patients

Contact Info

Product

Resources

About