Broiler welfare is preserved by long-term low-dose oral exposure to zinc oxide nanoparticles: preliminary study

Mahmoud, Magdy S.; Yahia, Doha; Abdel-Magiud, Doaa S.; Darwish, Madeha; Abd-Elkareem, Mahmoud; Mahmoud, Usama M.

doi:10.1080/17435390.2021.1905099

Cited by 11 publications

(5 citation statements)

References 51 publications

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“…There was no signi cant difference (p>0.05) in the glucose, total protein, albumin, cholesterol, ALT, uric acid, calcium and phosphorus values between the treatment groups. Total Protein, Total Bilirubin, Aspartate aminotransferase (AST) and Alanine Transaminase (ALT), ALP, uric acid levels, creatinine and globulin were not signi cantly affected by ZnONPs supplementation (Abdel-Monem et al, 2021; Mahmoud et al, 2021). Zinc concentration in serum was elevated dose dependently.…”

Section: Discussionmentioning

confidence: 99%

Effects of Zinc Oxide Nanoparticles on Growth, Carcass Characteristics and Intestinal Tight Junction Protein Gene Expression in 35-day old Broiler Chickens

Mozhiarasi

Karunakaran

Radhakrishnan

et al. 2022

Preprint

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In the present study, the effect of zinc oxide nanoparticles (ZnONPs) at different concentrations (20, 40 and 60 mg/kg) in broiler chicken diet on growth, carcass characteristics and intestinal health of broiler chicks were evaluated. The ZnONPs synthesized by physical method was characterized for its nanoparticle properties and evaluated for its in vitro toxicity using different cell lines. In vivo feeding trials was conducted with 150-day-old broiler chicks randomly assigned to five dietary treatments in three replicates for the period of 35 days with ZnONPs. The results indicated that ZnONPs supplemented group showed significantly higher (p < 0.05) body weight gain and the lower feed conversion ratio (FCR) compared to control. The carcass characteristics like meat pH and dressing percentage revealed the significance differences in the ZnONPs supplemented group. The intestinal histomorphology revealed significantly higher (p < 0.05) crypt depth in ileum and villi length to crypt depth ratio in duodenum of ZnONPs supplemented group. The zinc elemental concentration in the serum found to be significantly higher and the mRNA expression of intestinal tight junction protein genes like mucin-2 and claudin-3 found to be significantly upregulated in the ZnONPs supplemented group. It could be concluded that supplementation of ZnONPs in broiler diet could improve production performance, intestinal health status and can be used as an effective feed additive in broilers.

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Section: Discussionmentioning

confidence: 99%

Effects of Zinc Oxide Nanoparticles on Growth, Carcass Characteristics and Intestinal Tight Junction Protein Gene Expression in 35-day old Broiler Chickens

Mozhiarasi

Karunakaran

Radhakrishnan

et al. 2022

Preprint

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“…Наночастинки (НЧ) металів, в тому числі Цинку, можна успішно використовувати для задоволення потреб у мінеральних речовинах тварин і птиці, вони здатні підвищувати швидкість росту та використання ними корму (Mahmoud et al, 2021). Відомо, що наночастинки цинку оксиду (ZnO-NPs) переважають звичайні джерела цинку за фармакологічною активністю й біодоступністю, позитивно впливають на продуктивність і антиоксидантний захист, підвищують репродуктивний потенціал, проте потребують детального з'ясування токсикологічних параметрів (Khodaei-Motlagh et al, 2022).…”

Section: вступunclassified

Acute toxicity of zinc carbonate nanocrystals on white mice model

Koshevoy,

Naumenko,

Orobchenko

et al. 2023

SMLNUVMB

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Zinc-based nanoparticles (NPs) are successfully used to meet the mineral needs of animals and poultry, capable of increasing the growth rate and the efficiency of their use of feed. Nanoparticles of zinc oxide are widely used, which are superior to conventional sources of zinc in terms of pharmacological activity and bioavailability, have a positive effect on productivity and antioxidant protection, and increase reproductive potential. However, a detailed investigation of their toxicological parameters showed the risks of their introduction into the body, as a result of which a large number of zinc oxide NPs were synthesized by the methods of “green chemistry”, the main drawback of which is a complex methodology and a high level of costs. We investigated the acute toxicity of a colloidal solution of zinc carbonate nanocrystals in a model of white mice. The experiment was conducted on 42 female non-linear white mice, 3–4 months old, with a live weight of 21–23 g, kept under optimal vivarium conditions. According to the principle of analogues, 6 experimental groups were formed: mice were injected with a colloidal solution of zinc carbonate nanoparticles in doses (by absolute weight of the drug) of 15,000.0; 20000.0; 25000.0; 30000; 35000.0 and 40000.0 mg/kg of body weight based on the absolute weight of the drug, which according to the active substance (zinc carbonate nanoparticles) was (37.5; 50.0; 62.5; 75.0; 87.5 and 100, 0 mg/kg of body weight), orally using an esophageal-gastric tube. Mice of the control group were injected with distilled water in a volume of 0.2 cm3 according to the same protocol. The clinical condition of the experimental animals was observed for 14 days, noting the appearance and development of clinical signs of poisoning, the time of death or recovery to the physiological norm. After the death (diagnostic slaughter) of the animals, a pathological autopsy was performed. Clinical observations showed that a single intragastric administration of the drug to mice of experimental groups 1-6 did not cause acute poisoning. The deaths of mice in all experimental groups were not observed during the 14-day observation period, as a result of which the LD50 indicator could not be calculated. At the same time, the maximum possible injected dose of the colloidal solution of zinc carbonate nanoparticles (by absolute weight) was 40,000.0 mg/kg of body weight (100.0 mg/kg by active substance), which allows the drug to be assigned to the VI class of toxicity – substances are relatively harmless (LD50 > 15000.0 mg/kg of body weight), and according to the degree of danger up to IV class – low-hazardous substances (LD50 > 5000.0 mg/kg of body weight).

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“…An indirect exposure to ZnO NPs in commercial products is highlighted in the literature where the intake of ZnO NPs from canned sea food (fish) is reported to be around 109 μg/kg for adults. ZnO NPs are widely used as zinc supplements in food and agriculture industries (Youn and Choi, 2022; Mahmoud et al, 2021). In the near future, there will be an increase in the industrial release of ZnO NPs into the environment (Engin, 2021).…”

Section: Introductionmentioning

confidence: 99%

Zinc oxide nanoparticles trigger dysfunction of mitochondrial respiratory complexes and repair dynamics in human alveolar cells

et al. 2023

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Zinc oxide nanoparticles (ZnO NP) are commonly used engineered NPs with extensive usage in consumer products, thus leading to direct exposure to humans. The direct route of exposure is through inhalation. Once inhaled, these particles accumulate in the lungs, increasing the chances of respiratory tract illness through cellular organelle damage. Zinc oxide nanoparticle-treated lung cells are reported to display cytotoxicity, increase DNA damage, and induce oxidative stress. The current study focused on the effects of ZnO NPs on mitochondrial dynamics (fission and fusion) in human lung epithelial cells (A549). The lung cells were exposed to ZnO NPs at 50 and 100 μg/ml concentrations, and their mitochondrial dynamics were assessed to understand the effects of the NPs. Treatment with ZnO NPs reduced the activity of mitochondrial complex I and complex III and altered mitochondrial structural and functional characteristics in a concentration-dependent manner. Zinc oxide nanoparticles exposure showed an increase in small and round-shaped mitochondria. The expression of various fission proteins (Drp1 and Fis1) and fusion proteins (Mfn1, Mfn2, and OPA1) was altered upon exposure to ZnO NPs. Our studies showed dysfunction of the mitochondria induced by ZnO NPs. In fibroblast mitochondrial dynamics, fission symbolizes threshold damage. In this paper, we have shown that the mitochondrial fission phenotype increased upon exposure to ZnO NPs. The paper emphasizes that these particles enter mitochondria, triggering a stress response that results in the removal of mitochondria via fission. It provides relevant data for safety guidelines to ensure the safer use of these particles.

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Broiler welfare is preserved by long-term low-dose oral exposure to zinc oxide nanoparticles: preliminary study

Cited by 11 publications

References 51 publications

Effects of Zinc Oxide Nanoparticles on Growth, Carcass Characteristics and Intestinal Tight Junction Protein Gene Expression in 35-day old Broiler Chickens

Effects of Zinc Oxide Nanoparticles on Growth, Carcass Characteristics and Intestinal Tight Junction Protein Gene Expression in 35-day old Broiler Chickens

Acute toxicity of zinc carbonate nanocrystals on white mice model

Zinc oxide nanoparticles trigger dysfunction of mitochondrial respiratory complexes and repair dynamics in human alveolar cells

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