Broiler chickens, broiler chicken meat, pigs and pork as sources of ExPEC related virulence genes and resistance in Escherichia coli isolates from community-dwelling humans and UTI patients☆

Jakobsen, Lotte; Spangholm, Daniel J.; Pedersen, Karl; Jensen, Lars; Emborg, Hanne‐Dorthe; Agersø, Yvonne; Aarestrup, Frank Møller; Hammerum, Anette M.; Frimodt‐Møller, Niels

doi:10.1016/j.ijfoodmicro.2010.06.025

Cited by 122 publications

(85 citation statements)

References 43 publications

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“…This was in agreement with many previous studies that reported the genetic relatedness between APEC and human ExPEC isolates with an explanation of the origin similarity of both isolates along with the possession of common virulence and antimicrobial resistance genes [22][23][24][25]. Meanwhile, some other studies previously isolated O78 serotypes from stools of diarrheic patients [26][27][28].…”

Section: Discussionsupporting

confidence: 92%

Detection of phenotypes, virulence genes and phylotypes of avian pathogenic and human diarrheagenic Escherichia coli in Egypt

Ramadan

Awad

Ateya

2016

J Infect Dev Ctries

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Introduction: The purpose from this study was to determine phenotypes, intestinal virulence-associated genes, and phylotypic profiling of human diarrheagenic E. coli (DEC) and avian pathogenic E. coli (APEC). Methodology: A total of 108 chicken visceral organs (liver, spleen, heart) from 36 diseased birds (three organs per each bird) and 78 human stool samples (50 diarrheic patients and 28 healthy persons) were randomly collected during the first half of 2015 in the district of Mansoura city, Egypt. Conventional culturing, serotyping, and molecular characterization of virulence genes and phylogroups were performed. Results: Sixty-five (35%) biochemically identified E. coli isolates were detected from chicken visceral (29/108; 26.9%) and human stool samples (36/78; 46.2%). Serotypes O78, O2, and O1 were the most prevalent serotypes (62%) distinguished from APEC isolates, and only two similar serotypes (O119:H4 and O26:H11) were identified from both APEC and DEC isolates. By polymerase chain reaction (PCR), the respective percentages of 100 and 35 with eae and Shiga toxin genes were detected from APEC isolates while 50%, 27.8%, and 19.4% of human DEC isolates harbored eae, stx1, and stx2 genes, respectively. Phylogrouping revealed a significantly higher occurrence of pathogenic phylogroups (D and B2) in APEC (19/29; 65.5%) than in human DEC isolates (8/36; 22.2%). Conclusions: APEC isolates shared serotypes, virulence genes, and phylotypes with human DEC isolates, which is a subsequent potential public health concern. To the best of our knowledge, this is the first report in Egypt that determines virulence gene and phylogroup coexistence between APEC and DEC isolates.

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Section: Discussionsupporting

confidence: 92%

Detection of phenotypes, virulence genes and phylotypes of avian pathogenic and human diarrheagenic Escherichia coli in Egypt

Ramadan

Awad

Ateya

2016

J Infect Dev Ctries

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“…Here, phylogroup distribution varied in relation not only to pathotype (ExPEC versus non-ExPEC) but also source (meat versus eggs). We determined that the major phylotypes of ExPEC chicken isolates are similar to those of ExPEC isolates that cause human disease, which belong mainly to the B2 and D phylogroups, while non-ExPEC isolates belong predominantly to groups A and B1, which are more associated with fecal isolates than disease isolates (36)(37)(38). Human isolates from phylogroups B2 and D typically contain more virulence factors than do those from groups A and B1 (39).…”

Section: Discussionmentioning

confidence: 99%

Zoonotic Potential of Escherichia coli Isolates from Retail Chicken Meat Products and Eggs

Mitchell

Johnson

Johnston

et al. 2015

Appl Environ Microbiol

138

140

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c Chicken products are suspected as a source of extraintestinal pathogenic Escherichia coli (ExPEC), which causes diseases in humans. The zoonotic risk to humans from chicken-source E. coli is not fully elucidated. To clarify the zoonotic risk posed by Ex-PEC in chicken products and to fill existing knowledge gaps regarding ExPEC zoonosis, we evaluated the prevalence of ExPEC on shell eggs and compared virulence-associated phenotypes between ExPEC and non-ExPEC isolates from both chicken meat and eggs. The prevalence of ExPEC among egg-source isolates was low, i.e., 5/108 (4.7%). Based on combined genotypic and phenotypic screening results, multiple human and avian pathotypes were represented among the chicken-source ExPEC isolates, including avian-pathogenic E. coli (APEC), uropathogenic E. coli (UPEC), neonatal meningitis E. coli (NMEC), and sepsis-associated E. coli (SEPEC), as well as an undefined ExPEC group, which included isolates with fewer virulence factors than the APEC, UPEC, and NMEC isolates. These findings document a substantial prevalence of human-pathogenic ExPEC-associated genes and phenotypes among E. coli isolates from retail chicken products and identify key virulence traits that could be used for screening.

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“…However, in some conditions, they can cause either intestinal or extraintestinal infections. Manifestation of clinical symptomatology and pathology appears to be closely associated with the possession of certain virulence gene combinations that have a range of functions, including toxin production, attachment/invasion, and immune evasion [2][3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Diverse Escherichia coli pathovars of phylogroups B2 and D isolated from animals in Tunisia

Kilani

Abbassi

Ferjani

et al. 2017

J Infect Dev Ctries

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Introduction: The virulent Escherichia coli strains responsible for extraintestinal infections were mainly belonged to B2 and D phylogroups. However, no past studies have determinate via the presence of virulence genes the frequency of E. coli pathovars recovered from animals housed in farms in Tunisia. The aims of this study were to investigate 26 E. coli isolated from healthy and diarrheic animals and to determinate via the presence of virulence genes the frequency of pathovars. Methodology: Twenty-six E. coli isolates of phylogroups B2 (n = 14), B22 (n = 9), B23 (n = 5), and D2 (n = 12) were characterized. Genes encoding virulence factors (fimH,eaeA,aggC,papC, papG allele III, hlyA, east1, cnf1, exhA,stx1, stx2, iutA, fyuA, ibeA,and ipaH), and antibiotic resistance as well as class 1 and 2 integrons were searched by polymerase chain reaction (PCR). The genetic relationship of isolates was done by PFGE. Results: According to the occurrence of specific genes the 26 isolates were classified as:9 EAEC, 2 EHEC, 4 UPEC, 3 EPEC/EHEC and 1 NTEC. Therefore, 2 Ex-PEC and 5 APEC were presented amongst our strains. Some isolates (12) were clonal and the remaining was unrelated. Conclusions: Higher diversity of pathovars which carried diverse combinations of virulence genes in healthy isolates. In addition, it seems that the infections were caused by different mechanisms.

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Broiler chickens, broiler chicken meat, pigs and pork as sources of ExPEC related virulence genes and resistance in Escherichia coli isolates from community-dwelling humans and UTI patients☆

Cited by 122 publications

References 43 publications

Detection of phenotypes, virulence genes and phylotypes of avian pathogenic and human diarrheagenic Escherichia coli in Egypt

Detection of phenotypes, virulence genes and phylotypes of avian pathogenic and human diarrheagenic Escherichia coli in Egypt

Zoonotic Potential of Escherichia coli Isolates from Retail Chicken Meat Products and Eggs

Diverse Escherichia coli pathovars of phylogroups B2 and D isolated from animals in Tunisia

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