Broccoli-Derived Nanoparticle Inhibits Mouse Colitis by Activating Dendritic Cell AMP-Activated Protein Kinase

Abstract

The intestinal immune system is continuously exposed to massive amounts of nanoparticles derived from food. Whether nanoparticles from plants we eat daily have a role in maintaining intestinal immune homeostasis is poorly defined. Here, we present evidence supporting our hypothesis that edible nanoparticles regulate intestinal immune homeostasis by targeting dendritic cells (DCs). Using three mouse colitis models, our data show that orally given nanoparticles isolated from broccoli extracts protect mice against colitis. Broccoli-derived nanoparticle (BDN)-mediated activation of adenosine monophosphate-activated protein kinase (AMPK) in DCs plays a role in not only prevention of DC activation but also induction of tolerant DCs. Adoptively transferring DCs pre-pulsed with total BDN lipids, but not sulforaphane (SFN)-depleted BDN lipids, prevented DSS-induced colitis in C57BL/6 (B6) mice, supporting the role of BDN SFN in the induction of DC tolerance. Adoptively transferring AMPK, but not AMPK, DCs pre-pulsed with SFN prevented DSS-induced colitis in B6 mice, further supporting the DC AMPK role in SFN-mediated prevention of DSS-induced colitis. This finding could open new preventive or therapeutic avenues to address intestinal-related inflammatory diseases via activating AMPK.

Protective Role of Shiitake Mushroom-Derived Exosome-Like Nanoparticles in D-Galactosamine and Lipopolysaccharide-Induced Acute Liver Injury in Mice

“…Recently, dietary ELNs have been extracted from fruits (such as grapes [21], grapefruit [17,19], apples [33], and coconut water [45]); vegetables (such as carrots [21] and broccoli [31]); and spices (such as ginger [19,20,30] and turmeric [20]). However, it was not known whether edible mushrooms contained any ELNs.…”

“…To evaluate the effects of S-ELNs on acute liver injury induced by GalN/LPS, C57BL/6J mice were intraperitoneally injected with S-ELNs at the dose of 1 × 10 10 /g. The dose of S-ELNs was determined based on pilot pharmacokinetic tests and other groups' studies [29][30][31]. Then, 48 h later, mice were intraperitoneally injected with a mixed solution of GalN (Sigma, 34539, 500 mg/kg) and LPS (Sigma, L2630, 15 µg/kg) to trigger acute liver damage.…”

“…Dietary ELNs have been extracted from many edible plants, including fruits, vegetables, and spices [19,21,30,31,33]. To determine whether any edible mushrooms contained ELNs, seven commonly consumed mushrooms-white button, Swiss brown, king oyster, shiitake, white beech, brown beech, and oyster-were subjected to ELN extraction procedures established in our laboratory [20].…”

Investigation of the Cytotoxicity of Fig, Lemon, Olive, and Turnip Exosomes on MCF-7 Cells

“…Organosulphur compounds found in the turnip have been shown to decrease the proliferation of cancer cells (Farag and Motaal, 2010). In addition, broccoli-derived exosomes observed to inhibit mouse colitis (Deng et al, 2017). In this study, the observation that 100 µg/mL protein containing turnip exosomemediated inhibition of the proliferation of a breast cancer cell is unique for exosome-mediated growth inhibition of cancer cells, therefore this is an important contribution to the anticancer activities of cruciferous vegetables.…”

<p>Nanoparticle-Mediated Drug Delivery Systems For The Treatment Of IBD: Current Perspectives</p>

“…The capacity of other plant-derived NPs, including grape-, grapefruit-, and broccoli-derived NPs, to target different tissues is also likely to be mediated via multiple factors. 71,72,[99][100][101] These mechanisms warrant further study. Unlike the edible plant-derived NPs, most mammalian cell-derived NPs are not suitable for oral administration (see the injectable DDS section for more information on these NPs).…”