Broadly Neutralizing Monoclonal Antibodies to the V3 Region of HIV-1 Can Be Elicited by Peptide Immunization

Rabbit Anti-HIV-1 Monoclonal Antibodies Raised by Immunization Can Mimic the Antigen-Binding Modes of Antibodies Derived from HIV-1-Infected Humans

“…This limits the variation of the elbow angles in the antibodies, with 142°f or R56 and 154°for R20. Some rabbit antibodies were predicted to have an alternative disulfide bond between residues 108 and 170 (20). Our structures showed that this is possible as the distance between the C-␣ atoms of these two residues is ϳ5.6 Å (Fig.…”

“…7A). Mouse MAb 50.1, although also produced by immunization, was induced by using the disulfide-linked 40-mer cyclic peptide RP70 (INCTRPNYNK RKRIHIGPGR AFYTTKNIIG TIRQAHCNIS), which contains the V3 sequence of strain MN of HIV-1 gp120 (20). The complex structure of MAb 50.1 was resolved to 2.8 Å resolution by cocrystallizing its Fab with another cyclic peptide, MP1 (CKRIHIGPGR AFYTTC) (27).…”

“…There are many anti-V3 MAbs derived from human patients, as well as from animal models other than the rabbit (20)(21)(22)(23)(24). Most of the known anti-V3 MAbs are against the V3 crown; only a few of them target the C-terminal region, including a mouse MAb raised by immunization with a synthetic peptide (25).…”

A Recombinant Human Immunodeficiency Virus Type 1 Envelope Glycoprotein Complex Stabilized by an Intermolecular Disulfide Bond between the gp120 and gp41 Subunits Is an Antigenic Mimic of the Trimeric Virion-Associated Structure

“…The epitopes for, and some immunochemical properties of, anti-gp120 MAbs from various donors have been described previously (9,71,72). These include 19b and 83.1 to the V3 loop (74,118), IgG1b12 and F91 to the CD4 binding site (CD4bs) (15,72), 2G12 to a unique C3-V4 glycan-dependent epitope (108,109), M90 to the C1 region (113), 23A and sheep antibody D7324 to the C5 region (69,72), C11 to a discontinuous C1-C5 epitope (75), 17b to a CD4-inducible epitope (9,47,72,91,103,105,122,123), A32 to a CD4-inducible C1-C4 epitope (72,103), and G3-519 and G3-42 to C4 and C4/V3 epitopes, respectively (72,73). MAbs to gp41 epitopes included 7B2 to epitope cluster I (a gift from James Robinson); 4D4 to cluster I (11); T4, an oligomer-specific MAb overlapping the cluster I region (28); 2.2B to cluster II (a gift from James Robinson); T15G1 to cluster II (a gift from Ab Notkins); and 2F5 to a neutralizing epitope encompassing residues 653 to 659 (11,77,108).…”

Epitope mapping of the V3 domain of feline immunodeficiency virus envelope glycoprotein by monoclonal antibodies

“…That a principal neutralization domain exists within the third hypervariable region of the envelope protein of HIV-1 and HIV-2 is well established (for review see Wolf et al, 1993) and there are data indicating that such regions consist of multiple and/or discontinuous epitopes (White-Scharf et al, 1993;Seligman, 1994;Bj6rling et al, 1994).…”