In this work, a high-gain and wideband microstrip-fed slot antenna is proposed and investigated, which is composed of an anisotropic metasurface (AMS) and an aperture coupled structure. The proposed microstrip antenna with four resonances can be obtained by merging the AMS with an anomalous inverted π-slot feed structure in a low profile (1.07λ × 1.07λ × 0.06λ). The simulated results indicate that the proposed microstrip antenna can achieve a wide impedance bandwidth of 56.1% from 3.32 to 5.91 GHz, which is verified by experiment. In addition, the measured results show that the peak gain of the proposed microstrip-fed slot antenna is 10.7 dBi at 5.3 GHz, and the relative bandwidth of 3-dBi gain is 42.2% from 3.85 to 5.91 GHz. Compared with previous works, the proposed design has a lower profile while achieving a much wider operating bandwidth, where the four controllable resonance modes offer more possibilities for band expansion. This work shows potential application in integration with high data rate systems.